Scripts/notebooks to segment and track platelets in a growing thrombus
Current files:
fl.py
contains some utilities used in the notebooks, including objects for
opening the ND2 file and keeping track of various processed data products
(smoothing, thresholding, segmentation, etc.)
- The "process images" notebook is the first step, goes from images to
segmentation. It creates output files in a timestamped subfolder of the
current working directory.
- The "define variables" notebook then uses that segmentation to create tracks.
Goals:
- turn this from a set of loosely tied notebooks to a command-line utility that
runs various parts of the pipeline
- use zarr to save the original data and intermediate products
- use dask for processing where possible
- get the tracks into the napari tracks layer from napari/napari#1361
Installation:
git clone https://github.com/jni/pia-tracking.git
cd pia-tracking
conda env create -f environment.yml
conda activate platelet-tracking