i'm running mobster on a bam file.
the process stops and give me the follow error:
[Sun Feb 12 12:19:40 CET 2023] Executing as manuela@dell-mpro on Linux 5.15.0-58-generic amd64; OpenJDK 64-Bit Server VM 11.0.13+7-b1751.21; Picard version: 1.73(1234)
WARNING 2023-02-12 12:19:44 CollectInsertSizeMetrics All data categories were discarded because they contained < 0.05 of the total aligned paired data.
WARNING 2023-02-12 12:19:44 CollectInsertSizeMetrics Total mapped pairs in all categories: 0.0
[Sun Feb 12 12:19:44 CET 2023] net.sf.picard.analysis.CollectInsertSizeMetrics done. Elapsed time: 0.06 minutes.
Runtime.totalMemory()=262144000
Exit value: 0
0 [main] ERROR Mobster - ./prova_out_insertstats (No such file or directory)
what's the problem?
i tried to run samtools index on my bam file but it didn't help me.
i'm running mobster on a bam file. the process stops and give me the follow error:
[Sun Feb 12 12:19:40 CET 2023] Executing as manuela@dell-mpro on Linux 5.15.0-58-generic amd64; OpenJDK 64-Bit Server VM 11.0.13+7-b1751.21; Picard version: 1.73(1234) WARNING 2023-02-12 12:19:44 CollectInsertSizeMetrics All data categories were discarded because they contained < 0.05 of the total aligned paired data. WARNING 2023-02-12 12:19:44 CollectInsertSizeMetrics Total mapped pairs in all categories: 0.0 [Sun Feb 12 12:19:44 CET 2023] net.sf.picard.analysis.CollectInsertSizeMetrics done. Elapsed time: 0.06 minutes. Runtime.totalMemory()=262144000 Exit value: 0 0 [main] ERROR Mobster - ./prova_out_insertstats (No such file or directory)
what's the problem? i tried to run samtools index on my bam file but it didn't help me.
thank in advance