The Polygenic Risk Score Knowledge Base (PRSKB) is a website and command-line interface tool designed to facilitate the calculation of polygenic risk scores using genome-wide association studies from the NHGRI-EBI Catalog.
The PRSKB website offers users the ability to calculate polygenic risk scores across multiple traits with minimal effort. The website not only offers the calculator, but additional information and tools to aid in calculations and data comparisons.
Website Link: https://prs.byu.edu
The Calculate page of the PRSKB website allows the user to calculate polygenic risk scores for multiple samples. As of March 16, 2022, the PRSKB database contains the following data derived from the GWAS Catalog: 250,134 variant associations; 125,433 unique single nucleotide polymorphisms; 20,798 unique study and trait combinations; 10,366 GWA study identifiers; and 3,463 PubMed identifiers. The PRSKB is automatically updated with new studies from the GWAS Catalog monthly. Using an input VCF or inputted rsIDs and genotypes, reference genome, super population of the samples, p-value cutoff, and selected studies, polygenic risk scores can be calculated for the input samples. Results can be downloaded as a tsv or json file.
Note: Due to the vast number of studies the PRSKB has access to, it is not feasible to calculate PRS for them all on the website. If you wish to run large amounts of studies in a single run, see our Command-Line Interface download (CLI). Our current limit for number of studies to be run at a time is 500, although we strongly recommend using the CLI for anything more than 50 studies.
In addition to calculating polygenic risk scores using GWA studies from the GWAS Catalog stored in our database, users have the option to upload their own GWAS summary statistics to use in risk score calculations.
The GWAS summary statistics file to be uploaded must be in the correct format. It should be either a .tsv or a .txt tab separated file. The following columns are required and must be included in the file's header line: Study ID, Trait, RsID, Chromosome, Position, Risk Allele, Odds Ratio, P-value, and Super Population. If the summary statistics use beta values instead of odds ratios, replace the "Odds Ratio" column with two columns: "Beta Coefficients" and "Beta Units". Additional optional columns that will be included if present are: P-Value Annotation, Beta Annotation, Citation, and Reported Trait. Column order does not matter and there may be extra columns present in the file. Required and optional header names must be exact. Note that if P-value Annotation and/or Beta Annotation are present, then the calculator will separate calculations by those columns. If you do not wish for this to happen, do not include those optional columns.
If more than one odds ratio exists for an RsID/allele combination in a study, the tool will exit. Although we perform strand flipping on GWAS summary statistics data we use from the GWAS Catalog, we do not currently perform strand flipping on GWAS data uploaded to the website. Please ensure that your data are presented on the correct strand or use the CLI tool.
The Studies page of the PRSKB website includes a link to the published paper, information on citing the PRSKB, and the ability to search the database of GWAS studies procured from the GWAS Catalog.
The Download page houses the link to download the command-line interface tool and additional videos of setting up and running the tool.
The Visualize page allows users to view polygenic risk score distributions and statistics for 500,000 individuals from the UK Biobank, as well as from other cohorts including 1000 Genomes super populations and cohorts from the ADNI database. Since individual scores are only a relative metric to be understood in comparison to others within a population, this page allows for an approximate contextualization of scores received from the Calculate page.
The command-line interface (CLI) allows users to run larger analyses straight from their command-line. In addition to this, the tool has a built-in, interactive menu for searching studies and traits in the database, printing out available ethnicities from the database, and learning more about tool parameters.
Required installed programs: Bash and jq for bash, Python3 and the PyVCF, filelock, and requests Python modules
Note: PyVCF currently requires an additional step to install due to compatibility issues with setuptools v58+, which no longer supports use_2to3. PyVCF can be installed as follows:
pip uninstall pyvcf
pip install pyvcf./runPrsCLI.sh -f inputFile.vcf -o outputFile.tsv -r hg19 -c 0.05 -p EURMore CLI examples can be found in the README.md file included in the CLI download or on the readthedocs page for the tool.
There are two choices for the tsv output results - condensed (default) or full. Additonally, you can choose to output results in JSON format, which contains all the information found in the 'full' format. Explanations of the columns found in the output are given below.
This version of the output results contains one row for each study with columns for each sample's polygenic risk score. A column will be named using the samples identifier and that column will hold their risk scores.
Study ID | Reported Trait | Trait | Citation | P-Value Annotation | Beta Annotation | Score Type | Units (if applicable) | Used Super Population | SNPs Excluded Due To Cutoffs | SNP Overlap | Included SNPs | Sample1 | Sample2 | Sample3 | ect.
.. code-block:: bash
./runPrsCLI.sh -f path/to/file/samples.vcf -o path/to/file/output.tsv -c 0.0005 -r hg19 -p SAS
This version of the output results contains one row for each sample/study pair. It also includes columns listing the rsIDs of the snps involved in the risk score calculation.
Sample | Study ID | Reported Trait | Trait | Citation | P-Value Annotation | Beta Annotation | Score Type | Units (if applicable) | Used Super Population | SNPs Excluded Due To Cutoffs | SNP Overlap | Included SNPs | Polygenic Risk Score | Protective Variants | Risk Variants | Variants Without Risk Allele | Variants in High LD
.. code-block:: bash
./runPrsCLI.sh -f path/to/file/samples.vcf -o path/to/file/output.tsv -c 0.0005 -r hg19 -p SAS -v
This version outputs the results in a json object format. The output automatically contains all the data the full version does and there is no condensed version of the json output. The file will contain a list of json study objects. Each study object will contain the list of samples and their score.
.. code-block:: bash
[
{
"studyID": "GCST001",
"reportedTrait": "Alzheimer's Disease",
"trait": "Alzheimer Disease",
"citation": "First Author et al. 2021",
"pValueAnnotation": "NA",
"betaAnnotation": "NA",
"scoreType": "OR",
"units (if applicable)": "NA",
"excludedSnps": 3,
"usedSuperPop": "EUR",
"samples": [
{
"sample": "SAMP001",
"polygenicRiskScore": "1.277",
"protectiveAlleles": "rs1|rs2|rs3",
"riskAlleles": "rs4|rs5|rs6|rs7|rs8",
"variantsWithoutRiskAllele": "rs9|rs10|rs11|rs14",
"variantsInHighLD": "rs12|rs13",
"snpOverlap": 8,
"includedSnps": 14
},
{
"sample": "SAMP002",
"polygenicRiskScore": "NF",
"protectiveAlleles": "",
"riskAlleles": "",
"variantsWithoutRiskAllele": "rs1|rs2|rs3|rs4|rs5|rs6|rs7|rs8|rs9|rs10|rs11|rs14",
"variantsInHighLD": "rs12|rs13",
"snpOverlap": 8,
"includedSnps": 14
},
{
"sample": "SAMP003",
"polygenicRiskScore": "1.63",
"protectiveAlleles": "rs1|rs2|rs3",
"riskAlleles": "rs4|rs5|rs6|rs7|rs8",
"variantsWithoutRiskAllele": "rs1|rs2|rs3|rs4|rs5|rs6|rs7|rs8|rs9|rs10|rs11|rs14",
"variantsInHighLD": "rs12|rs13",
"snpOverlap": 8,
"includedSnps": 14
}
]
}
].. code-block:: bash
./runPrsCLI.sh -f path/to/file/samples.vcf -o path/to/file/output.json -c 0.0005 -r hg19 -p SAS
Please visit the Studies page of the website for information on how to cite this tool and GWAS publications used.
This work is freely available for academic and not-for-profit use. However, commercial use is regulated by © 2020 Brigham Young University. All rights reserved. For more information about commercial use of this product, please contact Justin Miller, Ph.D. (justin.miller@uky.edu) or Keoni Kauwe, Ph.D. (kauwe@byu.edu).