The hitager open source project provides a GUI based Windows application for writing and reading 125kHz automotive RFID key transponders commonly used in car keys. It uses an Arduino (software also provided in this project) as interface between a PCF7991 base station IC (ABIC) and a Windows PC running the AESHitager GUI. Additional infos can be found at the homepage of the project initiator's website.
AESHitager PC <-------> Arduino <-------> PCF7991 IC <- - - - -> Key Transponder
Detailed information on how to build the required hardware can be found in the project's Wiki