klavinslab / protocols_aquarium_1_0

All klavins lab protocols go here! (Except the ones that go in aqualib).

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Protocols

File Organization

Basic Cloning:
- Primer dilution
- Primer stock
- PCR
- Run Gel
- PCR purification
- Gel extraction
- Fragment stock
- Gibson
- E. coli transformation
  - Electrocompetent
  - Chemically competent
- E. coli overnight
- E. coli colony PCR
- Mini-prep
- Sequencing
  - Plasmid
  - Fragment
  - Colony
- Restriction digest
- Ligation
Yeast cloning
- Yeast transformation
- YPD streak
- Yeast colony PCR
- Loop out
- Gateway cloning
  - cDNA preperation
  - LR
  - BP
E. coli cloning
- Mage
- Keioe
Experiments:
- Cytomoter
  - Yeast assay
  - E. coli assay
- Plate reader
- Nandrop
  - DNA concentration
  - OD
- Microscope
- CellAsic -Device Use: (same things)
Device maintainance:
- Cytometer
- Plate reader
- Nanodrop
- Microscope
- -80 freezer
- -20 freezer
- 32 degree incubator
- 37 degree incubator
- 32 degree shaker
- 37 degree shaker
- Autoclaving
- Glass
- Plastic
Media/reagents/stocks:
- E. coli Media
  - Liquid
    - LB
    - M9 (Cellobiose, Glucose, PASC)
    - TB
  - Agar
    - LB
    - LB CMC
    - M9 (Cellobiose, Glucose, PASC)
- Yeast media
  - SOC
  - SC
  - YPD
- Reagents
  - Gibson
  - Competent cells
  - Yeast # Competent Yeast? Yeast Extract? What is this?
  - E. coli (NEB turbo, DH5alpha, MG1655)
Admin:
- Receiving
- Ordering
- Adding protocols
Training/safety:
- Basic lab training
- Pipette
- Lab organization
- Sterile technique
- Safety
- Waste disposal
- Clean-up -Acid/base safety -Emergency information
- Broken glass
- Spill
  - Acid/base
- Fire