Developed by Anand D. Patel in Vineet Bafna's lab at University of California, San Diego patel [dot] anandd [at] gmail [dot] com
AmBre is composed of two parts. A) Primer Designing B) Long read sequence analysis.
ambre_design <reference.fasta> <regions.txt> [<temptag>]
ambre_analyze <reference.fasta> <contig> <mapped_reads.sam> [<temptag>]AmBre requires Python 2.7.x and numpy
For primer designing, the following software needs to be installed
Primer3 Version 2.3.0 http://sourceforge.net/projects/primer3/
Blat Version 34x12 http://hgdownload.cse.ucsc.edu/admin/exe/
Multiplx Version 1.2 http://bioinfo.ut.ee/?page_id=167
Sequence analysis requires PacBio's BLASR and SMRT pipe.
AmBre can be run from source or installed.
Download the tar or zip ball and install using the setup.py script.
python setup.py installTo configure the above binary dependencies, edit "ambre.conf" file. The config file is located with
ambre_test -cEdit the file paths for primer3_dir, aligner_bin, and multiplx to locate the dependencies. For primer3_dir set the file path to the directory containing primer3_core. In the config file specify number of threads and a temp directory to store intermediate files. Note, user-specific config files can be used by running any module with the --config argument.
To verify the installation/configuration is correct and run a small test case for AmBre-design and AmBre-analyze (takes ~5min). ::
ambre_testIf all is well, continue to usage.
Alternatively, to run from source directory use ::
python -m ambre.test.install_unit -c
python -m ambre.test.install_unitAmBre-design and AmBre-analyze are fairly independent.
For AmBre-design see design usage for details. For AmBre-analyze see analyze usage for details.