Mixscale is an R package designed to analyze CRISPR interference (CRISPRi) based Perturb-seq data. It can quantify the heterogeneity of perturbation strength in each cell and improve the statistical power when doing differential expression (DE) analysis. It also provides functions for downstream analyses including decomposition, permutation test, gene set enrichment test, etc. A brief vignette is available at https://longmanz.github.io/Mixscale/ .
This package depends on several other R packages:
install.packages("Seurat")
install.packages("PMA")
install.packages("protoclust")
BiocManager::install("glmGamPoi")You can easily install the package by the following command:
install_github("longmanz/Mixscale")Our preprint is available at https://www.biorxiv.org/content/10.1101/2024.01.29.576933v2 .\ If you want to access the data generated in our paper (including the pathway gene signatures), you can download them at https://zenodo.org/records/10520190 .