The optimized configurations to capture short reads can not work well on PRO004RA data

novoalab / Nano-tRNAseq

Quantitative analysis of native tRNA populations using direct RNA nanopore sequencing (Lucas*, Pryszcz* et al., Nat Biotech 2023)

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The optimized configurations to capture short reads can not work well on PRO004RA data #10

Closed chenhx97 closed 4 months ago

chenhx97 commented 4 months ago

Hi! I've tried the MinKNOW alternative configurations to enhance short reads capture and it worked very well with R9.4.1 bulk data. However when I ran a sequencing simulation with PRO004RA bulk data using the same configurations, the output got worse instead. Is there any advice or update about it? Thanks for your time!