About this repo

Analysis of tRNA abundances and modifications using native RNA nanopore sequencing

This repository contains:

• conf/: MinKNOW alternative configurations enhanced capture of tRNA reads
• ref/: reference files
• src/: source code

Multiplexing Nano-tRNAseq runs

Yes, it is possible, both for SQK-RNA002 and SQK-RNA004 kits. Please take a look here.

Citation

If you find this work useful, please cite:

Lucas MC, Pryszcz L, Medina R, Milenkovic I, Camacho N, Marchand V, Motorin Y, Ribas de Pouplana L and Novoa EM. Quantitative anaysis of native tRNA populations using direct RNA nanopore sequencing. Nat Biotechnol (2023). https://doi.org/10.1038/s41587-023-01743-6