Hi there, this is a great package and concept. I can get the tutorial to run smoothly, but have trouble with my data.
I have ImmunoSEQ data which I (seemingly) succesfully import via the import_immunoseq_pipeline function. This generates an input that contains the mandatory columns (CDR3.amino.acid.sequence, bestVGene, bestJGene, Read.count).
However when I run the ALICE_pipeline I get the error:
Error in 1:nrow(VJlist) : argument of length 0
Does this mean there are no significant hits? How do I trace where the issue is?
Hi there, this is a great package and concept. I can get the tutorial to run smoothly, but have trouble with my data.
I have ImmunoSEQ data which I (seemingly) succesfully import via the import_immunoseq_pipeline function. This generates an input that contains the mandatory columns (CDR3.amino.acid.sequence, bestVGene, bestJGene, Read.count).
However when I run the ALICE_pipeline I get the error: Error in 1:nrow(VJlist) : argument of length 0
Does this mean there are no significant hits? How do I trace where the issue is?
Thank you.