rtviii / riboxyz

htpps://ribosome.xyz
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Landmarks #71

Open rtviii opened 4 months ago

rtviii commented 4 months ago

This is a place to keep track of desirable landmarks and protocol for grounding them.

Helices

Strategy: ...

There doesn't seem to be a straightforward way to do this short of aligning everything to handpicked locations from 2014, which is not so terrible idea in the end. Waiting to chat with Anton.

PTC (Peptidyl Transferase Center)

Strategy: ... The active site where peptide bond formation occurs.

Decoding Center: Where codon-anticodon recognition takes place.

Strategy: ...

mRNA entry and exit sites

Strategy: ...

tRNA binding sites: A-site (aminoacyl), P-site (peptidyl), and E-site (exit)

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Structural Elements:

ASL (Anticodon Stem Loop): Part of the tRNA that interacts with the mRNA codon.

Strategy: ...

L7/L12 Stalk (also known as L7/L11 stalk in some organisms):

Important for factor binding and GTPase activation.

Strategy: ...

CP (Central Protuberance): A prominent feature of the large subunit.

Strategy: ...

Intersubunit Bridges: Connections between the small and large subunits.

Strategy: ...

Exit Tunnel: The path through which the nascent peptide leaves the ribosome.

Strategy: ...

Specific Regions:

Sarcin-Ricin Loop (SRL): A highly conserved rRNA element important for factor binding.

Strategy: ...

GAC (GTPase-Associated Center): Involved in GTPase activation of translation factors.

Strategy: ...

530 Loop: A conserved element in the small subunit involved in decoding.

Strategy: ...

790 Loop: Another important element in the decoding process.

Strategy: ...

Dynamic Elements:

Head of the small subunit: Undergoes significant movement during translation. Strategy: ... L1 Stalk: Involved in tRNA movement through the ribosome. Strategy: ...

Factor Binding Sites:

EF-Tu binding site

Strategy: ...

EF-G binding site

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Release factor binding sites

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Expansion Segments: Additional rRNA sequences found in eukaryotes but not in prokaryotes.