sars-cov-2-variants / lineage-proposals

Repository to propose and discuss lineages
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Multiple EG.5.1* Lineages with S:L455F #537

Closed NkRMnZr closed 11 months ago

NkRMnZr commented 1 year ago

### Multiple EG.5.1\* Lineages with S:L455F or S:L455S
- [x] [Branch 1](#branch_1): Designated as HK.8 via [cov-lineages/pango-designation@`9527e15`](https://github.com/cov-lineages/pango-designation/commit/9527e15afb0acedf720e1042d5da347a4c0abd7d) , after transferred to cov-lineages/pango-designation#2230 , Phylogenetic order follows: EG.5.1.1 > C11779T > `G22927T`(**S:L455F**)
- [ ] [Branch 2](#branch_2): Transferred to cov-lineages/pango-designation#2229 EG.5.1.1 > C12053T(ORF1a:L3930F) > C503T(ORF1a:P80S) > C7732T, C18175T(ORF1b:P1570S), `G22927T`(**S:L455F**)
- [x] [Branch 3](#branch_3): Designated as **HK.14** via [cov-lineages/pango-designation@`c7550b1`](https://github.com/cov-lineages/pango-designation/commit/c7550b14adc5cff2e855903e3841458886c3073c) , after transferred to cov-lineages/pango-designation#2194 , Phylogenetic order follows: EG.5.1.1 > C11779T > C6781T, C17802T, `G22927C`(**S:L455F**)
- [x] [Branch 4](#branch_4): Designated as **EG.5.1.8** via [cov-lineages/pango-designation@`072c887`](https://github.com/cov-lineages/pango-designation/commit/072c887d266647163602965c26d74a389ae8175a) after transferred to cov-lineages/pango-designation#2224 , Phylogenetic order follows: EG.5.1 > `G22927T`(**S:L455F**)
- [x] [Branch 5](#branch_5): Designated as **HK.13** via [cov-lineages/pango-designation@`49cdcb4`](https://github.com/cov-lineages/pango-designation/commit/49cdcb4cc5fe60cf6826fbdb9580ccedf22d3e33) , after transferred to cov-lineages/pango-designation#2250 , Phylogenetic order follows: EG.5.1.1 > `G22927C`(**S:L455F**)
- [ ] [Branch 7](#branch_7): ~~EG.5.1.1 > C6627T(ORF1a:T2121I) > T17147C(ORF1b:I1227T) > `G22927T`(**S:L455F**) [Query: `C6627T, T17147C, G22927T`, 51 seqs, 01/19]~~ Slow
- [ ] [Branch 8](#branch_8): EG.5.1.1 > C5284T > T14655C > C15951T, `G22927T`(**S:L455F**) [Query:`C5284T, T14655C, G22927T`, 68 seqs, 01/18]
- [ ] [Branch 9](#branch_9): **WITHDRAWN ex-HK.10 (EG.5.1.1.10)** via [cov-lineages/pango-designation@`f655671`](https://github.com/cov-lineages/pango-designation/commit/f6556710dd5e198e827b227bd3db24ae11b45c74) Phylogenetic order follows: EG.5.1.1 > C19983T > `G22927T`(**S:L455F**) ⚠️ No Longer tracking, there are now a lot of **HK.3** sublineages with `C19983T`, this seems weirdly homoplastic.
- [ ] [Branch 10](#branch_10): Retracted from cov-lineages/pango-designation#2251 EG.5.1.1 > C11173T > `G22927T`(**S:L455F**) [Query: `C11173T, G13627A, G22927T, -T10837G, -T15042C, -T22228C, -C27406T, -T28157C`, 739 seqs, 01/19]
- [ ] [Branch 11](#branch_11): EG.5.1 > C9430T > C20873G(ORF1b:A2469G), `G22927T`(**S:L455F**) [Query: `C9430T, G21718T, G22927T, -C1889T, -G13627A, -C21622A`, 53, 01/12]
- [x] [Branch 12](#branch_12): Designated as **EG.5.1.17**, phylogenetic order follows: EG.5.1 > `G22927C`(**S:L455F**) [Query: `C2334T, A18492G, G22927C, -G1069A, -A22106C, -C22570T, -C25572T`]
- [ ] [Branch 13](#branch_13): Transferred to cov-lineages/pango-designation#2364 EG.5.1.1 > C19011T > C8991T(ORF1a:A2909V) > `G22927T`(**S:L455F**)
- [ ] [Branch 14](#branch_14): ~~EG.5.1.1 > A8443G > `G22927T`(**S:L455F**) [Query: `C6541T, A8443G, G22927T, -T4579A`, 7 seqs, 10/12]~~ Dead
- [x] [Branch 15](#branch_15): Designated with three levels: **HK.23**, **HK.23.1** and **HK.23.1.1** after Transferred to cov-lineages/pango-designation#2340 EG.5.1.1 > C28087T(ORF8:A65V) > `G22927T`(**S:L455F**), many with further `S:A348S`
- [ ] [Branch 16](#branch_16): ~~EG.5.1.1 > C8074T, T17811C > C7604T, C10789T, C10834T, C12815T, G20348T(ORF1b:S2294I), `G22927C`(**S:L455F**), A25998G [Query: `C6541T, C8074T, G22927C`, 13 seqs, 01/19]~~ Slow
- [ ] [Branch 17](#branch_17): Transferred to cov-lineages/pango-designation#2341 EG.5.1.1 > C9118T > G16813T(ORF1b:V1116L), `G22927T`(**S:L455F**)
- [x] [Branch 20](#branch_20): Designated **HK.33**, phylogenetic order follows: EG.5.1.1 > C2644T > T7957C, `G22927T`(**S:L455F**), G29348T(N:A359S) [Query: `C2644T, G13627A, G22927T`]
- [x] [Branch 23](#branch_23): Designated as **HK.2.1** via [cov-lineages/pango-designation@`d75dabe`](https://github.com/cov-lineages/pango-designation/commit/d75dabedbd433e944eeaee05222453dbda071cf1) after transferred to cov-lineages/pango-designation#2319 , phylogenetic order follows: **HK.2 (EG.5.1.1.2)** > G22927T(**S:L455F**)
- [x] [Branch 24](#branch_24): Designated as **`HK.20`** via [cov-lineages/pango-designation@`71cf97b`](https://github.com/cov-lineages/pango-designation/commit/71cf97b211299abf8f997396b40419285016b435) after transferred to cov-lineages/pango-designation#2353 , phylogenetic order follows: EG.5.1.1 > C6695T(ORF1a:P2144S) > C9448A > C5575T, G22927T(**S:L455F**)
- [ ] [Branch 25](#branch_25): EG.5.1.1 > C29296T > G22927T(**S:L455F**) [Query: `C6541T, G22927T, C29296T`, 70 seqs, 01/17] (Mostly with `S:A684V`)
- [x] [Branch 26](#branch_26): Designated as **HK.11.1** via [cov-lineages/pango-designation@`e268dbf`](https://github.com/cov-lineages/pango-designation/commit/e268dbfb10735703aeec95cf82092020689f4f4d) after transferred to cov-lineages/pango-designation#2320 , phylogenetic order follows **HK.11** > T13228C > T4789A > G22927T(**S:L455F**)
- [ ] [Branch 27](#branch_27): Transferred to cov-lineages/pango-designation#2342 EG.5.1.1 > C25493A(ORF3a:T34K) > G28884C(**N:G204P**) > G22927T(**S:L455F**)
- [ ] [Branch 28](#branch_28): Transferred to cov-lineages/pango-designation#2398 EG.5.1.1 > C5812T > G22927T(**S:L455F**) > A26565C(M:K15Q), C28770G(N:T166R)
- [ ] [Branch 31](#branch_31): Transferred to cov-lineages/pango-designation#2321 , follows EG.5.1.1 > C12020T(ORF1a:L3919F), T24496C > C2232T(ORF1a:A656V), C21516T, G22927T(**S:L455F**)
- [ ] [Branch 32](#branch_32): EG.5.1.1 > T4579A > G2329A, A8443G > G22927T(**S:L455F**) [Query: `T4579A, A8443G, G22927T`, 73 seqs, 01/17]
- [x] [Branch 33](#branch_33): Designated as **EG.5.1.19** after transferred to cov-lineages/pango-designation#2322 , phylogenetic order follows EG.5.1 > T26171A(ORF3a:M260K) > A17615G(ORF1b:K1383R), G22927T(**S:L455F**)
- [ ] [Branch 34](#branch_34): ~~EG.5.1.3 > G2946A(ORF1a:G894D), T15264C, T22926C(**S:L455S**) [Query: `C1889T, C2334T, T22926C, -A4340G, -A10703C`, 12 seqs, 01/03]~~ Slow
- [ ] [Branch 35](#branch_35): Transferred to cov-lineages/pango-designation#2354 EG.5.1.4 > C26111T(ORF3:P240L) > C18555T > T17268C > G22927T(**S:L455F**)
- [ ] [Branch 36](#branch_36): Transferred to cov-lineages/pango-designation#2399 EG.5.1.1 > C22388T > C1853T, G22927T(**S:L455F**)
- [ ] [Branch 37](#branch_37): ~~EG.5.1.1 > C29095T > G22927T(**S:L455F**)~~ `C29095T` became homoplastic across the EG.5.1+L455F tree, no longer tracking.
- [ ] [Branch 38](#branch_38): EG.5.1.1 > T24583C > G22927T(**S:L455F**) [Query: `C6541T, G22927T, T24583C`, 47 seqs, 01/19]
- [ ] [Branch 39](#branch_39): Designated as **HK.1.1** and retracted , Phylogenetic order follows: HK.1 (EG.5.1.1.1) > G22927T(**S:L455F**) [Query: `C6541T, G13627A, G22332, G22927T`, 132 seqs, 01/19] this query already included further mutated `S:G257D`
- [ ] [Branch 40](#branch_40): EG.5.1.1 > C10615T > G22927T(**S:L455F**) > C1426T, G25691T(ORF3a:G100V) [Query: `C1426T, C10615T , G22927T`, 48 seqs, 01/19]
- [ ] [Branch 41](#branch_41): ~~EG.5.1.1 > C24023T > C2485T, G15025A(ORF1b:A520T), G22927C(**S:L455F**) [Query: `C6541T, G22927C, C24023T`, 15 seqs, 01/17]~~ Slow
- [ ] [Branch 42](#branch_42): Transferred to cov-lineages/pango-designation#2355 EG.5.1.1 > C22687T > G22927T(**S:L455F**)
- [ ] [Branch 43](#branch_43): ~~EG.5.1.1 > C19524T > G22927T(**S:L455F**), A28254Δ~~ Seems pulled under HK.3, no longer tracking
- [x] [Branch 44](#branch_44): Designated with 3 levels: **EG.5.1.11**, **JR.1** and **JR.1.1**, Phylogenetic order follows: EG.5.1 > A22106C(**S:K182Q**) > C22995G(**S:T478R**), A25664G(ORF3a:Y91C) > C1613T(ORF1a:L450F), G20995T(ORF1b:G2510C), G22927C(**S:L455F**), A27388C
- [ ] [Branch 45](#branch_45): Transferred to cov-lineages/pango-designation#2308 EG.5.1.1 > C13968A, A15885G > T11449C > C10369T, G22927T(**S:L455F**), C29077T, G29140T(N:Q289H)
- [x] [Branch 46](#branch_46): Designated as **HK.22** via [cov-lineages/pango-designation@`2d3115a`](https://github.com/cov-lineages/pango-designation/commit/2d3115af565faf391755d033d9802bc717e3dd51) , phylogenetic order follows: EG.5.1.1 > G25407T(ORF3a:M5I) > C2662T > G22927T(**S:L455F**) > T15408C, T24709C > T2698C, A3019G, G18332A(ORF1b:R1622K), T22917A(**S:L452Q**) [Query: `C2662T, G22927T, G25407T`]
- [ ] [Branch 47](#branch_47): EG.5.1.1 > C7600T > G22927T(**S:L455F**) > T10216C [Query: `C7600T, T10216C, G22927T`, 52 seqs, 01/15]
- [ ] [Branch 48](#branch_48): Transferred to cov-lineages/pango-designation#2360 EG.5.1.1 > A23539T > G22927T(**S:L455F**) ⚠️ Please check potential misplacement.
- [ ] [Branch 49](#branch_49): ~~EG.5.1.4 > C8407T, C11008A(ORF1a:H3581Q), G22927T(**S:L455F**), G25538T(ORF3a:G49V), C25572T [Query: `A18492G, A21137G, G22927T, -C6120T, -G13627A, -C26111T`, 76 seqs, 01/20]~~ Now at least 3 smaller clusters seems pulled together a `S:L455F` branch right under EG.5.1.4 polytomy, hard to catch by proper query now.
- [ ] [Branch 50](#branch_50): ~~EG.5.1.1 > G28904T(N:A211S) > G22927T(**S:L455F**), G27261T, C28744T [Query: `C6541T, G22927T, G28904T`, 27 seqs, 01/19]~~ Slow
- [ ] [Branch 51](#branch_51): Transferred to cov-lineages/pango-designation#2365 EG.5.1.1 > C10015T > G22927T(**S:L455F**)
- [ ] [Branch 53](#branch_53): Transferred to cov-lineages/pango-designation#2366 EG.5.1.1 > C27741T > C1912T, C1968T(ORF1a:T568I), T7111C, A20010T(ORF1b:Q2181H), T20592C, G22927C(**S:L455F**), C28093T(ORF8:S67F)
- [ ] [Branch 54](#branch_54): EG.5.1.1 > G24368T(**S:D936Y**) > G22927T(**S:L455F**) [Query: `G13627A, G22927T, G24368T, -G3567A, -C4012T, -C5031T, -T13234C, -C17976T`, 72 seqs, 01/19]
- [x] [Branch 55](#branch_55): Designated with 2 levels: **HK.24** and **HK.24.1** , phylogenetic order follows EG.5.1.1 > G23522A(**S:E654K**) > G22927T(**S:L455F**)
- [ ] [Branch 56](#branch_56): EG.5.1.1 > A22582G > G22927T(**S:L455F**) [Query: `G13627A, A22582G, G22927T, -G3153A`, 39 seqs, 01/16]
- [ ] [Branch 57](#branch_57): EG.5.1.1 > A22582G > G3153A(ORF1a:G963D) > G22927T(**S:L455F**) [Query: `G3153A, A22582G, G22927T`, 57 seqs, 01/17]
- [ ] [Branch 58](#branch_58): EG.5.1 > T26972C > C28909T > C2106A(ORF1a:T614N), G28423T(ORF9b:R47L) > A21759G(**S:H66R**), G22927T(**S:L455F**), G24004A, C25572T [Query: `A18492G, G22927T, C28909T`, 46 seqs, 01/19]
- [ ] [Branch 59](#branch_59): EG.5.1.1 > G20134T(ORF1b:V2223L) > G22927T(**S:L455F**) [Query: `G13627A, G20134T, G22927T`, 46 seqs, 01/18] ⚠️ Please check potential reversion/artifact/misplacement
- [ ] [Branch 60](#branch_60): EG.5.1.1 > T16545G > C1471T, G22927T(**S:L455F**) [Query: `G13627A, T16545G, G22927T`, 67 seqs, 01/19]
- [ ] [Branch 62](#branch_62): ~~EG.5.1.1 > C18559T(ORF1b:L1698F) > T15927C, G22927T(**S:L455F**) [Query: `C6541T, C18559T, G22927T`, 16 seqs, 01/16]~~ Slow
- [ ] [Branch 63](#branch_63): Transferred to cov-lineages/pango-designation#2400 EG.5.1.1 > T14034C > T6556C, G22927T(**S:L455F**) ⚠️ Please check potential misplacement
- [ ] [Branch 64](#branch_64): EG.5.1.6 > C28236T(ORF8:R115C) > C16092T > C10969T > G526T(ORF1a:E87D) > C23380T > A12485G(ORF1a:I4074V) > T877C, A15270T(ORF1b:E601D), G22927T(**S:L455F**) [Query: `G526T, A15270T, G22927T`, 35 seqs, 01/19], thanks to @aviczhl2 and @FedeGueli
- [ ] [Branch 65](#branch_65): EG.5.1 > A3210G(ORF1a:D982G) > G26888A >G26227T, C28720T > T8909C> G22927T(**S:L455F**) [Query: `A3210G, T8909C, G22927T`, 25 seqs, 01/19]
- [ ] [Branch 66](#branch_66): EG.5.1.1 > C11779T > C12525T(ORF1a:T4087I) > G22927T(**S:L455F**) [Query: `C11779T, C12525T, G22927T`, 45 seqs, 01/19]
- [ ] [Branch 67](#branch_67): EG.5.1.1 > C25613T(ORF3a:S74F) > G22927C(**S:L455F**) [Query: `C6541T, G22927C, C25613T, -C26681T, -G26840A`, 51 seqs, 01/16]
- [ ] [Branch 68](#branch_68): EG.5.1.1 > G16710A > G22927T(**S:L455F**) [Query: `G16710A, G13627A, G22927T, -G28904T`, 72 seqs, 01/19]
- [ ] [Branch 69](#branch_69): EG.5.1.1 > G1085T(ORF1a:V274L) > G1535A(ORF1a:V424I), G22927T(**S:L455F**) [Query: `G1085T, G13627A, G22927T, -A11000G, -C29375T`, 69 seqs, 01/20]
- [ ] [Branch 70](#branch_70): EG.5.1.1 > T21748C > A16225G(ORF1b:T920A), T25036C > G22927C(**S:L455F**) [Query: `A16225G, T21748C, G22927C`, 42 seqs, 01/19]
- [ ] [Branch 71](#branch_71): EG.5.1.1 > C6638T(ORF1a:H2125Y) > G22927T(**S:L455F**) [Query: `C6638T, G13627A, G22927T, -C14889T`, 28 seqs, 01/19]
- [ ] [Branch 72](#branch_72): EG.5.1.1 > C11779T > G10261A > C9611T(ORF1a:L3116F), C18348T > G22927T(**S:L455F**) [Query: `C9611T, C11779T, G22927T`, 47 vseqs, 01/19]
- [ ] [Branch 73](#branch_73): HK.28 (C19170T) > G22927T(**S:L455F**) [Query: `G13627A, C19170T, G22927T, -C1909T, -C3736T, -C5031T, -C7504T, -A18153G`, 57 seqs, 01/19]
- [ ] [Branch 74](#branch_74): ~~EG.5.1.3 > A28032G(ORF8:I47V) > A1047C(ORF1a:K261T) > C3608A(ORF1a:L1115I), C18261T, G22927T(S:L455F) [Query: `A1047C, C1889T, G22927T`, 6 seqs, 11/22]~~ dead
- [ ] [Branch 75](#branch_75): EG.5.1.1 > C29253T(N:S327L) > G22927T(**S:L455F**) [Query: `G13627A, G22927T, C29253T, -G1067T, -C2110T, -A2730C`, 38 seqs, 01/19] ⚠️ Please check potential misplacement
- [ ] [Branch 76](#branch_76): HK.30 (EG.5.1.1 + T26609C) > C5907T(ORF1a:T1881I), T17454A > G22927T(**S:L455F**) [Query: `G13627A, G22927T, T26609C, -C24616T`, 40 seqs, 01/16]
- [x] [Branch 77](#branch_77): Designated **HV.2** after transferred to cov-lineages/pango-designation#2401 EG.5.1.6 > C925T, C27161T > C14919A > G14559A > G22927T(**S:L455F**)
- [ ] [Branch 78](#branch_78): EG.5.1.1 > C18395T(ORF1b:A1643V) > G22927T(**S:L455F**) [Query: `C6541T, C18395T, G22927T`, 47 seqs, 01/16]
- [ ] [Branch 79](#branch_79): ~~EG.5.1.1 > T6736C > T23287C > C22075T > C8389T, A16668G, C17474T(ORF1b:T1336I), G22927T(S:L455F), G26416T(E:V58F) [Query: `T6736C, G13627A, G22927T`, 16 seqs, 01/04] (Mostly with `S:Q14H/G21604C`)~~ Slow
- [ ] [Branch 80](#branch_80): EG.5.1.1 > C8146T > G2035A, G6373T(ORF1a:M2036I), G22927T(**S:L455F**) [Query: `C8146T, G13627A, G22927T, -C27557T`, 19 seqs, 01/17]
- [ ] [Branch 81](#branch_81): EG.5.1.1 > C2857T > G22927T(**S:L455F**) [Query: `C2857T, G13627A, G22927T, -C5031T, -T17475C`, 50 seqs, 01/19]
- [ ] [Branch 82](#branch_82): EG.5.1.1 > T16494C, T22307C > G22927C(**S:L455F**) [Query: `G13627A, T16494C, G22927C`, 18 seqs, 01/17]
- [ ] [Branch 83](#branch_83): EG.5.1.1 > C29358T(N:T362I) > T23803C > C842T(ORF1a:P193S), C15141T, T16743C, C22450T, G22927T(**S:L455F**), C24442T, C25916T(ORF3a:T175I), C28232T [Query: `G22927T, T23803C, C29358T`, 28 seqs, 01/10], thanks to @aviczhl2
- [ ] [Branch 84](#branch_84): HK.19 > C2455T > G22927T(**S:L455F**), C23533T [Query: `C2455T, C22033A, G22927T, -G25606A`, 20 seqs, 01/19]
- [ ] [Branch 85](#branch_85): EG.5.1.1 > C7390T > G22927T(**S:L455F**) [Query: `C7390T, G13627A, G22927T`, 49 seqs, 01/15] Mostly with `ORF6:N34K/C27303A`
- [ ] [Branch 86](#branch_86): EG.5.1.1 > G25855T(ORF3a:D155Y) > G22927T(**S:L455F**) > T4063C, C13405T, A21612C(**S:N17T**) [Query: `A21612C, G22927T, G25855T`, 79 seqs, 01/29]
- [ ] [Branch 87](#branch_87): EG.5.1 > C15960T > C16260T > G22927T(**S:L455F**), C24771T(**S:A1070V**), C25658T(ORF3a:T89I) [Query: `C15960T, C16260T, A18492G, G22927T`, 19 seqs, 01/19]
- [ ] [Branch 88](#branch_88): EG.5.1.1 > T4579A > A4576T > G22927T(**S:L455F**), [Query: `A4576T, G13627A, G22927T`, 118 seqs, 01/29] (Sibling to **Branch 32**)
- [ ] [Branch 89](#branch_89): EG.5.1.1 > C27406T(ORF7a:L5F) > G22927T(**S:L455F**) [Query: `C6541T, G13627A , G22927T, C27406T`, 11 seqs, 01/08]
- [ ] [Branch 90](#branch_90): EG.5.1.1 > C13724T(ORF1a:A4487V), A25636T(ORF3a:N82Y) > T10750C, G22927T(**S:L455F**) [Query: `G13627A, C13724T, G22927T, -C5031T`, 28 seqs, 01/12]
- [ ] [Branch 91](#branch_91): EG.5.1.1 > C22987T >G22927T(**S:L455F**) > G19419A, G21974C(**S:D138H**) [Query: `G13627A, G19419A, G21974C`, 12 seq, 12/26]
- [ ] [Branch 92](#branch_92): EG.5.1.1 > C629T(ORF1a:L122F) > A1168G > C22987T > T595C, C20148T, G22927T(**S:L455F**) [Query: `A1168G, G22927T, C22987T`, 11 seqs, 01/09]
- [ ] [Branch 93](#branch_93): ~~EG.5.1.18 > T1216C, A9034G, A12121T, A12519G(ORF1a:D4085G), G22927T(**S:L455F**) [Query: `G9269A, G22927T, G26051T`, 8 seqs, 12/19]~~ Slow
- [ ] [Branch 94](#branch_94): EG.5.1.13 > _G670T_rev(ORF1a:R135S_rev)_ > T28861C > G8889A(ORF1a:R2875H) > C13381T > G22424T(**S:A288S**) > C25047T(**S:P1162L**) > G25855T(ORF3a:D155Y) > G22927T(**S:L455F**) [Query: `G8889A, C13381T, G22927T`, 49 seqs, 01/15], a sub-branch of #1074 proposed by @aviczhl2
- [x] Designated with 2 levels: **HK.27** and **HK.27.1** from cov-lineages/pango-designation#2226 proposed by @FedeGueli , phylogenetic order follows: EG.5.1.1 > C5031T(ORF1a:T1589I) > `G22927T`(**S:L455F**) [Query: `C5031T, G22927T`]
- [x] Designated as **HK.1.2** via [cov-lineages/pango-designation@`2ee937c`](https://github.com/cov-lineages/pango-designation/commit/2ee937ccb13e3ee615171bdf04dde0ebf453807a) , formerly doublet, now proposed by @aviczhl2 : cov-lineages/pango-designation#2310 , Phylogenetic order follows: HK.1 (EG.5.1.1.1) > G6320A(ORF1a:V2019I) > C11704T, C22482T(**S:T307I**), G22927C(**S:L455F**) [Query: `G6320A, G22332T, G22927C`]
- [x] Designated as **JJ.1 (EG.5.1.4.1)** via [cov-lineages/pango-designation@`36e3fb0`](https://github.com/cov-lineages/pango-designation/commit/36e3fb099782e00f74e2ea4d9843cebe5aa48b60) , cov-lineages/pango-designation#2208 formerly a singlet, proposed by @aviczhl2 , Phylogenetic order follows: EG.5.1.4 > C26111T(ORF3:P240L) > G22927T(**S:L455F**), A25531G(ORF3a:I47V) [Query: `A21137G, G22927T, A25531G`]
- [x] Designated as **JG.3 (EG.5.1.3.3)** via [cov-lineages/pango-designation@`b084562`](https://github.com/cov-lineages/pango-designation/commit/b084562b0354573bcca5b27064caa06cdf04982c), proposed by @aviczhl2 , cov-lineages/pango-designation#2231 Phylogenetic order follows: EG.5.1.3 > C7086T(ORF1a:T2274I), G22927T(**S:L455F**), C23673T(**S:S704L**) [Query: `C1889T, G22927T, C23673T`]
- [ ] cov-lineages/pango-designation#2434 proposed by @aviczhl2 , Phylogenetic order follows: **EG.5.1.1** >T14988C, A23014T(S:A484A) > C1471T > C12823T > G25459T(ORF3a:A23S) > G22927T(**S:L455F**), ins28214T [Query: `C1471T, T14988C, G22927T`, 89 seqs, 01/19]
- [x] HK.3 (EG.5.1.1.3) designated via [cov-lineages/pango-designation@`39a35ba`](https://github.com/cov-lineages/pango-designation/commit/39a35ba57d785e6bdda763fd612b9d7ba4442119) , Phylogenetic order follows: EG.5.1.1 > `G22927T`(**S:L455F**)

Branch 1:

Transferred to main repo. Designated as HK.8 **Designated as HK.8 (EG.5.1.1.8) via cov-lineages/pango-designation@9527e15** _Transferred to cov-lineages/pango-designation#2230_ **Defining Mutations:** `EG.5.1.1` > `C11779T` > `G22927T(S:L455F)` **Query:** `C6541T, C11779T, G22927T` **Earliest seq:** 2023-06-27 (`EPI_ISL_18060870`, Yibin, Sichuan, China) **Latest seq:** 2023-07-17 (`EPI_ISL_18060877`, Zi'yang, Sichuan, China) **Sampled Countries:** China (2, Sichuan)
Genomes: `EPI_ISL_18060870, EPI_ISL_18060877`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_202eb_c2cf60.json?f_userOrOld=uploaded%20sample ![Branch1](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/45712d3a-99c1-444f-90a5-d54903ff70dc)

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Branch 2:

Transferred to main repo: _Transferred to cov-lineages/pango-designation#2229_ **Defining Mutations:** `EG.5.1.1` > `C12053T(ORF1a:L3930F)` > `C503T(ORF1a:P80S)` > `C7732T, C18175T(ORF1b:P1570S), G22927T(S:L455F)` **Query:** `C503T, C6541T, G22927T` **Earliest seq:** 2023-07-15 (`EPI_ISL_18060497`, Tokyo, Japan) **Latest seq:** 2023-07-18 (`EPI_ISL_18065046`, Miyagi, Japan) **Sampled Countries:** Japan (2)
Genomes: `EPI_ISL_18060497, EPI_ISL_18065046`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_22c78_c2e570.json?f_userOrOld=uploaded%20sample ![Branch2](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/9b39313e-98f9-4892-a3d8-48effc0f6abe)

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Branch 3

Transferred to main repo _Transferred to cov-lineages/pango-designation#2194_ **Defining Mutations:** `EG.5.1.1` > `C11779T` > `C6781T, C17802T, G22927C(S:L455F)` **Query:** `C6781T, C17802T, G22927C` **Earliest seq:** 2023-07-04 (`EPI_ISL_17994488`, Shanghai, China) **Latest seq:** 2023-07-18 (`EPI_ISL_18074754`, Shanghai, China) **Sampled Countries:** China (3, Shanghai)
Genomes: `EPI_ISL_17994488, EPI_ISL_18074751, EPI_ISL_18074754`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_13292_36d050.json?f_userOrOld=uploaded%20sample ![Branch3](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/40e19529-9315-4dc4-aa04-126e4e38e0f8)

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Branch 4

Transferred to main repo Transferred to cov-lineages/pango-designation#2224 **Defining Mutations:** `EG.5.1` > `C12100G, G22927T`(**S:L455F**) **Query:** `C12100G, G22927T` **Earliest seq:** 2023-07-09 (`EPI_ISL_18060822`, Shanghai, China) **Latest seq:** 2023-07-31 (`EPI_ISL_18094969`, Washington, USA with Travel History to: Japan) **Sampled Countries:** China (2, Shanghai, 1 ex. Thailand), USA (2, Washington, ex. Japan)
Genomes: `EPI_ISL_18060822, EPI_ISL_18075026, EPI_ISL_18094946, EPI_ISL_18094969`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_1ebd2_7501c0.json?f_userOrOld=uploaded%20sample&label=id:node_6794320 ![Branch4](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/09a0e977-4ba2-47a4-9f39-728b807afd46)

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Branch 5

Transferred to main repo _Transferred to cov-lineages/pango-designation#2250_ **Defining Mutations:** `EG.5.1.1` > `G22927C`(**S:L455F**, different to **HK.3**'s `G22927T`) **Query:** `C6541T, G22927C, -C3653T, -C6317T, -C6327T, -A6659G, -T7111C, -C8074T, -G8768A, -C10702T, -C11779T, -C13968A, -T17811C, -A21137G, -T21748C, -C22482T, -C24023T, -C27741T, -G29734T` **Earliest seq:** 2023-07-08 (`EPI_ISL_18012368`, Sichuan, China) **Latest seq:** 2023-07-27 (`EPI_ISL_18105834`, Shanghai, China) **Sampled Countries:** China (3: Shanghai/2; Sichuan/1)
Genomes: `EPI_ISL_18012368, EPI_ISL_18074450, EPI_ISL_18105834`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_a8d5_ccb160.json?branchLabel=nuc%20mutations&f_userOrOld=uploaded%20sample&label=id:node_6794516 ![Branch5](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/7121e114-52de-42ff-88d3-2a565bee4f43)

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Branch 6

Many sequences pulled back to HK.3, the reversion probably due to dropout rather than recombinant **Defining Mutations:** `EG.5.1.1` > _T22480C_Reversion_ > `G22927T`(**S:L455F**) **Query:** `C6541T, G13627A, G22927T, -C7948T, -C22480T` **Earliest seq:** 2023-07-05 (`EPI_ISL_17988079`, Xiangyang, Hubei) **Latest seq:** 2023-08-07 (`EPI_ISL_18105560`, Xiangyang, Hubei) **Sampled Countries:** China (4, Hubei)
Genomes: `EPI_ISL_17988079, EPI_ISL_18105559-18105560, EPI_ISL_18105562`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_100ff_ccdc70.json?f_userOrOld=uploaded%20sample&label=id:node_6791410 ![Branch6](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/0e84c4f9-ccf6-431b-85be-02749568d1e8)

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Branch 7

Defining Mutations: EG.5.1.1 > C6627T(ORF1a:T2121I) > T17147C(ORF1b:I1227T) > G22927T(S:L455F) Query: C6627T, T17147C, G22927T Earliest seq: 2023-07-06 (EPI_ISL_18074610, Shanghai) Latest seq: 2023-08-05 (EPI_ISL_18108436, Shanghai, Travel history: Singapore) Sampled Countries: China (3: Shanghai)

Genomes: `EPI_ISL_18074610, EPI_ISL_18108436, EPI_ISL_18108490`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_1999b_eabba0.json?f_userOrOld=uploaded%20sample&label=id:node_10741278 Branch7

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Branch 8

Defining Mutations: EG.5.1.1 > C5284T > T14655C > C15951T, G22927T(S:L455F) Query: C5284T, T14655C, G22927T Earliest seq: 2023-07-23 (EPI_ISL_18105571, Shanghai) Latest seq: 2023-08-11 (EPI_ISL_18114886, Shanghai) Sampled Countries: China (3, Shanghai)

Genomes: `EPI_ISL_18105571, EPI_ISL_18108625, EPI_ISL_18114886`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_28a92_b9eb0.json?f_userOrOld=uploaded%20sample&label=id:node_6895881 Branch8

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Branch 9

Defining Mutations: EG.5.1.1 > C19983T > G22927T(S:L455F) Query: C6541T, G13627A, C19983T, G22927T, -C6695T **Earliest seq:** 2023-06-30 (EPI_ISL_18074254, Liaoning, China) **Latest seq:** 2023-08-27 (EPI_ISL_18239976`, Utah, USA) Sampled Countries: China (3: Liaoning/2; Shanghai/1)

Genomes: `EPI_ISL_18074249, EPI_ISL_18074254`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_2cc80_bc030.json?f_userOrOld=uploaded%20sample&label=id:node_6895986 Branch9

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Branch 10

Transferred to main repo _Transferred to cov-lineages/pango-designation#2251_ **Defining Mutations:** EG.5.1.1 > C11173T > G22927T(**S:L455F**) **Query:** `C11173T, G13627A, G22927T, -T22228C, -C27406T, -T28157C` **Earliest seq:** 2023-07-16 (`EPI_ISL_18049850`, Qinghai) **Latest seq:** 2023-08-01 (`EPI_ISL_18114762`, Shanghai) **Sampled Countries:** China (3, Qinghai, Shanghai, Xinjiang)
Genomes: `EPI_ISL_18049850, EPI_ISL_18108872, EPI_ISL_18114762`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_33d9d_bf590.json?f_userOrOld=uploaded%20sample&label=id:node_10744521 ![Branch10](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/812b2eab-80c7-41a0-b88e-c829a85b3569)

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Branch 11

Defining Mutations: EG.5.1 > C9430T > T1939C, C10039T, C20873G(ORF1b:A2469G), G22927T(S:L455F) Query: C9430T, G21718T, G22927T, -C1889T, -G13627A, -C21622A Earliest seq: 2023-07-31 (EPI_ISL_18127303, Tokyo, Japan) Latest seq: 2023-08-07 (EPI_ISL_18127606, Kanagawa, Japan) Sampled Countries: Japan (3: Tokyo/2; Kanagawa/1)

Genomes: `EPI_ISL_18127215, EPI_ISL_18127303, EPI_ISL_18127606`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice4_genome_1293d_792f00.json?f_userOrOld=uploaded%20sample Branch11

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Branch 12:

Designated EG.5.1.17 _Designated as **EG.5.1.17** via cov-lineages/pango-designation@5a8103c_ **Defining Mutations:** `EG.5.1` > `G22927C(S:L455F)` **Query:** `C2334T, A18492G, G22927C, -G1069A, -A22106C, -C22570T, -C25572T` **Earliest seq:** 2023-07-09 (`EPI_ISL_18108295`, Anhui, China) **Latest seq:** 2023-08-15 (`EPI_ISL_18138165`, Shanghai, China) **Sampled Countries:** China (4: Shanghai/3; Anhui/1)
Genomes: `EPI_ISL_18108295, EPI_ISL_18114797, EPI_ISL_18138165, EPI_ISL_18138282`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_289ba_a2e580.json?f_userOrOld=uploaded%20sample&label=id:node_6920519 ![Branch12](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/265b77e9-fb98-487a-ae1a-fe2014bed801)

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Branch 13:

Transferred to main repo _transferred to cov-lineages/pango-designation#2364_ **Defining Mutations:** `EG.5.1.1` > `C19011T` > `C8991T(ORF1a:A2909V)` > `G22927T(S:L455F)` **Query:** `C8991T, C19011T, G22927T` **Earliest seq:** 2023-08-14 (`EPI_ISL_18146054`, Hubei, China) **Latest seq:** 2023-10-31 (`EPI_ISL_18473645`, Singapore, travel history: India) **Sampled Countries:** China (38: HB/23; SH/4; AH/3; BJ/2; YN/2; GS/1; HA/1; QH/1; XJ/1), Singapore (5: Local/2; ex. China/2; ex. India/1), Netherlands (3), Australia (2: NSW), Austria (1), Croatia (1), France (1), Laos (1)
Genomes: `EPI_ISL_18141179, EPI_ISL_18146054, EPI_ISL_18146087, EPI_ISL_18217222, EPI_ISL_18217236, EPI_ISL_18254205, EPI_ISL_18268148-18268149, EPI_ISL_18268153, EPI_ISL_18268244, EPI_ISL_18284712, EPI_ISL_18284755, EPI_ISL_18306934, EPI_ISL_18313098, EPI_ISL_18313105, EPI_ISL_18313198, EPI_ISL_18313226, EPI_ISL_18349877, EPI_ISL_18354962, EPI_ISL_18363349, EPI_ISL_18376465, EPI_ISL_18376494, EPI_ISL_18384477, EPI_ISL_18384508, EPI_ISL_18388887, EPI_ISL_18388889, EPI_ISL_18388891, EPI_ISL_18438085, EPI_ISL_18438311, EPI_ISL_18438356, EPI_ISL_18438359, EPI_ISL_18438427, EPI_ISL_18441693, EPI_ISL_18442727, EPI_ISL_18442730, EPI_ISL_18447747, EPI_ISL_18457659, EPI_ISL_18461703, EPI_ISL_18461971, EPI_ISL_18463327, EPI_ISL_18463426, EPI_ISL_18464919, EPI_ISL_18464984, EPI_ISL_18465193, EPI_ISL_18465381, EPI_ISL_18465391, EPI_ISL_18466345, EPI_ISL_18473414, EPI_ISL_18473645, EPI_ISL_18475163, EPI_ISL_18475178`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_16441_b42970.json?label=id:node_3500994 ![image](https://github.com/cov-lineages/pango-designation/assets/125747944/6656c336-7805-4491-8fc0-8072605bf90a)

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Branch 14

Defining Mutations: EG.5.1.1 > A8443G > G22927T(S:L455F) Query: C6541T, A8443G, G22927T Earliest seq: 2023-07-22 (EPI_ISL_18060879 Ziyang, Sichuan) Latest seq: 2023-08-09 (EPI_ISL_18146071, Xiaogan, Hubei) Sampled Countries: China (3: Sichuan/2; Hubei/1)

Genomes: `EPI_ISL_18060879, EPI_ISL_18146071, EPI_ISL_18166195`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_140e3_db750.json?f_userOrOld=uploaded%20sample Branch14

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Branch 15

Designated with levels: HK.23, HK.23.1 and HK.23.1.1 Designated as three levels: [HK.23](https://github.com/cov-lineages/pango-designation/commit/12691b30210c9ab9b68ebfe04bda957d9a09b806), [HK.23.1](https://github.com/cov-lineages/pango-designation/commit/b83d9aa28a32b8a70a6e049b29986577cf07f23a) and [HK.23.1.1](https://github.com/cov-lineages/pango-designation/commit/b47c3ca2d6369c236951efde3b954e9ac022e5b7) _Transferred to cov-lineages/pango-designation#2340_ **Defining Mutations:** `EG.5.1.1` > `C28087T(ORF8:A65V)` > `G22927T(S:L455F)` **Query:** `C6541T, G22927T, C28087T, -A4306G, -G4444A, -A6889G, -G8171A, -T8305C, -C10039T, -C19185T, -C25207T` **Earliest seq:** 2023-08-10 (`EPI_ISL_18138246`, Shanghai) **Latest seq:** 2023-08-14 (`EPI_ISL_18208534`, Shanghai) **Sampled Countries:** China (3: Shanghai)
Genomes: `EPI_ISL_18138040, EPI_ISL_18138246, EPI_ISL_18208534`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_192a8_20dc90.json?f_userOrOld=uploaded%20sample ![Branch15](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/bf26d8a6-4591-49a8-a8e4-1dba0e1f51de)

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Branch 16

Defining Mutations: EG.5.1.1 > C8074T > T17811C > C7604T, C10789T, C10834T, C12815T, G20348T(ORF1b:S2294I), G22927C(S:L455F), A25998G Query: C6541T, C8074T, G22927C Earliest seq: 2023-08-22 (EPI_ISL_18217203, Hubei, China) Latest seq: 2023-08-22 (EPI_ISL_18217204, Hubei, China) Sampled Countries: China (3)

Genomes: `EPI_ISL_18217202-18217204`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_1c2cf_6feba0.json?c=gt-S_455&gmax=25384&gmin=21563&label=id:node_6949824 Branch16

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Branch 17

Transferred to main repo _Transferred to cov-lineages/pango-designation#2341_ **Defining Mutations:** `EG.5.1.1` > `C9118T` > `G22927T(S:L455F)` **Query:** `C6541T, C9118T, G22927T, -C25546A` **Earliest seq:** 2023-08-14 (`EPI_ISL_18214798`, Osaka, Japan) **Latest seq:** 2023-10-02 (`EPI_ISL_18373543`, England, UK) **Sampled Countries:** Japan (37), USA (4: TX/2; NJ; SC), South Korea (2), UK (2: EN), Australia (1: WA), Canada (1: ON), France (1)
Genomes: `EPI_ISL_18162837, EPI_ISL_18166739, EPI_ISL_18214798, EPI_ISL_18214838, EPI_ISL_18226559, EPI_ISL_18235760, EPI_ISL_18236014, EPI_ISL_18242568, EPI_ISL_18243896, EPI_ISL_18247826-18247827, EPI_ISL_18253490, EPI_ISL_18261506, EPI_ISL_18271898, EPI_ISL_18279175, EPI_ISL_18279234, EPI_ISL_18285272, EPI_ISL_18285440, EPI_ISL_18294200, EPI_ISL_18300272, EPI_ISL_18338985, EPI_ISL_18342460, EPI_ISL_18354762, EPI_ISL_18354776, EPI_ISL_18362312, EPI_ISL_18370698, EPI_ISL_18370761, EPI_ISL_18371874, EPI_ISL_18372806, EPI_ISL_18373146, EPI_ISL_18373543, EPI_ISL_18376280, EPI_ISL_18376286, EPI_ISL_18377931, EPI_ISL_18382356, EPI_ISL_18382437, EPI_ISL_18382737, EPI_ISL_18385259, EPI_ISL_18387636, EPI_ISL_18390707, EPI_ISL_18390782, EPI_ISL_18390836, EPI_ISL_18397002, EPI_ISL_18402105, EPI_ISL_18402112, EPI_ISL_18411313, EPI_ISL_18413797, EPI_ISL_18413896`
**UShER:** https://nextstrain.org/fetch/genome-test.gi.ucsc.edu/trash/ct/subtreeAuspice1_genome_test_1f50e_2e6ab0.json?f_userOrOld=uploaded%20sample&label=id:node_3461917 ![image](https://github.com/cov-lineages/pango-designation/assets/125747944/9bf07613-bbac-4302-90a2-578ca67c3648)

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Branch 18

Merged into HK.3 **Defining Mutations:** `EG.5.1.1` > `C22264T` > `G22927T(S:L455F)` **Query:** `G13627A, C22264T, G22927T` **Earliest seq:** 2023-07-30 (`EPI_ISL_18129236`, British Columbia, Canada) **Latest seq:** 2023-08-28 (`EPI_ISL_18213253`, Fujian, China) **Sampled Countries:** Canada (1, BC), China (1, Fujian), UK (1, England)
Genomes: `EPI_ISL_18129236, EPI_ISL_18142517, EPI_ISL_18213253`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_2fbb2_705f80.json?c=gt-nuc_22264&gmax=23264&gmin=21264&label=id:node_6943899 ![image](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/0b91a73e-5147-4ac7-a21e-048eeb07efb0)

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Branch 19

Merged under HK.13, no longer tracking **Defining Mutations:** `EG.5.1.1` > `C29167T` > `G1186A, T4871C(ORF1a:Y1536H), C7011T(ORF1a:A2249V), G17741A(ORF1b:R1425H), G22927C(S:L455F)` **Query:** `T4871C, G17741A` **Earliest seq:** 2023-08-09 (`EPI_ISL_18160385`, New York, USA) **Latest seq:** 2023-08-19 (`EPI_ISL_18214905`, Ibaraki, Japan) **Sampled Countries:** China (1, Shanghai), Japan (1), USA (1, NY)
Genomes: `EPI_ISL_18160385, EPI_ISL_18208589, EPI_ISL_18214905`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_15b7_70e2f0.json?label=id:node_6948139 ![Branch19](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/0b622b0c-273c-4a0b-8b4a-2e1d8934f5e5)

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Branch 20

Designated as HK.33 _Designated as HK.33 via cov-lineages/pango-designation@7ad122e_ **Defining Mutations:** `EG.5.1.1` > `C2644T` > `T7957C, G22927T(S:L455F), G29348T(N:A359S)` **Query:** `C2644T, G13627A, G22927T` **Earliest seq:** 2023-08-21 (`EPI_ISL_18222131`, `SEA` airport, Washington, USA, travel history: UK) **Latest seq:** 2023-08-25 (`EPI_ISL_18208454`, Shanghai, China) **Sampled Countries:** USA (2, ex. UK), China (1, Shanghai)
Genomes: `EPI_ISL_18147035, EPI_ISL_18208454, EPI_ISL_18222131`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_172d3_85e8d0.json?f_userOrOld=uploaded%20sample&label=id:node_6951215 ![Branch20](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/477f1392-9f03-4288-82c4-0f61ab72f1ad)

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Branch 21, now pulled under HK.3.8

Pulled under HK.3.8 **Defining Mutations:** `EG.5.1.1` > `C18747T` > `C643T, T13060C, G19723T(ORF1b:V2086F), G22927T(S:L455F)` **Query:** `C643T, C18747T, G22927T` **Earliest seq:** 2023-08-11 (`EPI_ISL_18208543`, Shanghai) **Latest seq:** 2023-08-28 (`EPI_ISL_18232547`, Shanghai Customs, travel history to Japan **Sampled Countries:** China (3, SH)
Genomes: `EPI_ISL_18208543, EPI_ISL_18208548, EPI_ISL_18232547`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_1d67e_99d900.json?label=id:node_10811725 ![image](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/3c05a1f5-eb0c-4b18-8389-f5b9da65dab6)

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Branch 22, Now pulled under HK.3.2 > A708C(ORF1a:E148A) > C10969T, G23593T(S:Q677H), G28056A(ORF8:A55T), no longer tracking.

Pulled under HK.3.2 **Defining Mutations:** `HK.5` > `G22927T(S:L455F)` **Query:** `spike_Q52H, spike_L455F, spike_Q677H` **Earliest seq:** 2023-08-13 (`EPI_ISL_18146068`, Hubei) **Latest seq:** 2023-08-24 (`EPI_ISL_18226161`, Virginia) **Sampled Countries:** USA (2, VA), China (1, HB)
Genomes: `EPI_ISL_18146068, EPI_ISL_18226161-18226162`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_34c06_9a5d70.json?f_userOrOld=uploaded%20sample&label=id:node_10811724 ![image](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/e2f94f6f-d03f-49f5-bc6a-8238b685160d)

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Branch 23

Transferred to main repo: _Transferred to main repo cov-lineages/pango-designation#2319_ **Defining Mutations:** `HK.2` > `G22927T(S:L455F)` **Query:** `C6541T, G21604T, G22927T, -A518G, -C934T, -C1426T, -G3663A, -C5031T, -C7712T, -A7849G, -C8320T, -T16545G` **Earliest seq:** 2023-08-21 (`EPI_ISL_18141161`, Singapore) **Latest seq:** 2023-08-23 (`EPI_ISL_18232599`, Shanghai) **Sampled Countries:** Singapore (1), China (2, SH)
Genomes: `EPI_ISL_18141161, EPI_ISL_18232598-18232599`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_40e5c_9aa250.json?f_userOrOld=uploaded%20sample&label=id:node_10811725 ![image](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/77f25a80-600d-4b82-b157-89102858a070)

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Branch 24, Designated as HK.20 via cov-lineages/pango-designation@71cf97b ; follow-up S:G482R/G23006C designated as HK.20.1

Designated as HK.20 and HK.20.1 _Transferred to cov-lineages/pango-designation#2353_ **Defining Mutations:** `EG.5.1.1` > `C6695T(ORF1a:P2144S)` > `C9448A` > `C5575T, G22927T(S:L455F)` **Query:** `C6695T, C9448A, G22927T` **Earliest seq:** 2023-08-13 (`EPI_ISL_18114870`, Shanghai Customs, travel history to Japan) **Latest seq:** 2023-10-20 (`EPI_ISL_18448529`, Ontario, Canada) **Sampled Countries:** China (13, SH/6; SC/4; FJ/1; ex. Japan/1; ex. S. Korea/1), USA (12: CA/8; AZ/1; OH/1; TX/1; ex. Nepal/1), Singapore (6: Local/5; ex. China/1), Italy (5), UK (3: EN), Australia (2: NSW; QLD), Canada (2: ON), Japan (2), South Korea (2), Switzerland (2), Denmark (1), France (1), Spain (1)
Genomes: `EPI_ISL_18114870, EPI_ISL_18208555, EPI_ISL_18208592, EPI_ISL_18208601, EPI_ISL_18232643, EPI_ISL_18246292, EPI_ISL_18284706, EPI_ISL_18285031, EPI_ISL_18285192, EPI_ISL_18306956, EPI_ISL_18307066, EPI_ISL_18320793, EPI_ISL_18328004, EPI_ISL_18352555, EPI_ISL_18354001, EPI_ISL_18355023, EPI_ISL_18360344, EPI_ISL_18360616, EPI_ISL_18361723, EPI_ISL_18366403, EPI_ISL_18370066, EPI_ISL_18370200, EPI_ISL_18371411, EPI_ISL_18376528, EPI_ISL_18385607, EPI_ISL_18386446, EPI_ISL_18386489, EPI_ISL_18387133, EPI_ISL_18397207, EPI_ISL_18403093, EPI_ISL_18407761, EPI_ISL_18409112, EPI_ISL_18411495, EPI_ISL_18415804, EPI_ISL_18421982, EPI_ISL_18423702, EPI_ISL_18425050, EPI_ISL_18428715, EPI_ISL_18429030, EPI_ISL_18430106, EPI_ISL_18433978, EPI_ISL_18435244, EPI_ISL_18442967, EPI_ISL_18443714, EPI_ISL_18443742-18443744, EPI_ISL_18443746, EPI_ISL_18444154, EPI_ISL_18446641, EPI_ISL_18448416, EPI_ISL_18448529`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_11cc_c2b40.json?label=id:node_3485632 ![image](https://github.com/cov-lineages/pango-designation/assets/125747944/536ca3bf-307c-4008-9935-30f195bed8ac)

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Branch 25: Mostly with S:A684V

Defining Mutations: EG.5.1.1 > C29296T > G22927T(S:L455F) Query: C6541T, G22927T, C29296T Earliest seq: 2023-08-06 (EPI_ISL_18133933, Qinghai) Latest seq: 2023-08-30 (EPI_ISL_18245873, Gansu) Sampled Countries: China (4: SC/2; GS; QH)

Genomes: `EPI_ISL_18133933, EPI_ISL_18245873, EPI_ISL_18246316, EPI_ISL_18246328`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_fec3_1b4b0.json?label=id:node_10825408 image

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Branch 26

Transferred to main repo _Transferred to main repo cov-lineages/pango-designation#2320_ **Defining Mutations:** `HK.11` > `T13228C` > `T4789A` > `G22927T(S:L455F)` **Query:** `T4789A, T13228C, G22927T` **Earliest seq:** 2023-08-21 (`EPI_ISL_18221467`, Halland, Sweden) **Latest seq:** 2023-08-21 (`EPI_ISL_18244247`, Denmark) **Sampled Countries:** Denmark (2), Sweden (1)
Genomes: `EPI_ISL_18221467, EPI_ISL_18244233, EPI_ISL_18244247`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_1d161_22300.json?f_userOrOld=uploaded%20sample&label=id:node_6955887 ![image](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/8f89dd4e-c794-40a8-941f-91cb71dfa234)

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Branch 27

Transferred to main repo _Transferred to cov-lineages/pango-designation#2342_ **Defining Mutations:** `EG.5.1.1` > `C25493A(ORF3a:T34K)` > `G28884C(N:G204P)` > `G22927T(S:L455F)` **Query:** `C6541T, G22927T, C25493A` **Earliest seq:** 2023-07-18 (`EPI_ISL_18245992`, Heilongjiang) **Latest seq:** 2023-10-10 (`EPI_ISL_18397223`, Singapore) **Sampled Countries:** China (18: HL/10; LN/2; SH/2; HB; SC; SX; YN), Singapore (14), Denmark (4), Italy (3), USA (3: CA/2; NY), France (2), Australia (1: NSW), Germany (1), South Korea (1), Sweden (1)
Genomes: `EPI_ISL_18138073, EPI_ISL_18232576, EPI_ISL_18244264, EPI_ISL_18245964, EPI_ISL_18245971, EPI_ISL_18245992, EPI_ISL_18246139, EPI_ISL_18246193, EPI_ISL_18282065, EPI_ISL_18300796, EPI_ISL_18306948, EPI_ISL_18306976, EPI_ISL_18306993, EPI_ISL_18307005-18307006, EPI_ISL_18307019, EPI_ISL_18307026, EPI_ISL_18313136, EPI_ISL_18320696, EPI_ISL_18324230, EPI_ISL_18329168, EPI_ISL_18330265, EPI_ISL_18339790, EPI_ISL_18352920, EPI_ISL_18356975, EPI_ISL_18361976, EPI_ISL_18362045, EPI_ISL_18376532, EPI_ISL_18376955, EPI_ISL_18377536, EPI_ISL_18377636, EPI_ISL_18382895, EPI_ISL_18384393, EPI_ISL_18384413, EPI_ISL_18384418, EPI_ISL_18384426, EPI_ISL_18384448, EPI_ISL_18384625, EPI_ISL_18384643, EPI_ISL_18391206-18391207, EPI_ISL_18397223, EPI_ISL_18397241, EPI_ISL_18397267, EPI_ISL_18397584, EPI_ISL_18401938, EPI_ISL_18405209, EPI_ISL_18406218`
**UShER:** https://nextstrain.org/fetch/genome-test.gi.ucsc.edu/trash/ct/subtreeAuspice1_genome_test_58dd0_2eda30.json?f_userOrOld=uploaded%20sample&label=id:node_3458763 ![image](https://github.com/cov-lineages/pango-designation/assets/125747944/287e4c21-ad58-4ae8-8be9-f6b2b6b93270)

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Branch 28

Transferred to main repo: _Transferred to cov-lineages/pango-designation#2398_ **Defining Mutations:** **EG.5.1.1** > `C5812T` > `G22927T(S:L455F)`, mostly with >> `A26565C(M:K15Q), C28770G(N:T166R)` **Query:** `C5812T, G22927T, C28770G` (for major `A26565C(M:K15Q), C28770G(N:T166R)` sub-branch only) **Earliest seq:** 2023-08-09 (`EPI_ISL_18214077`, South Korea) **Latest seq:** 2023-11-09 (`EPI_ISL_18547984`, Nevada, USA) **Sampled Countries:** South Korea (68), USA (5: NV/2; NC/1; ex. S. Korea/2), Australia (2: NSW; QLD), China (2: SC/1; ex. S. Korea/1), Denmark (2), Hungary (2), Finland (1), Japan (1)
Genomes: `EPI_ISL_18214077, EPI_ISL_18236938, EPI_ISL_18293068, EPI_ISL_18293497, EPI_ISL_18347905, EPI_ISL_18353940, EPI_ISL_18354370, EPI_ISL_18354403, EPI_ISL_18355265, EPI_ISL_18355547, EPI_ISL_18368528, EPI_ISL_18382157, EPI_ISL_18382304, EPI_ISL_18382505, EPI_ISL_18382615, EPI_ISL_18386641, EPI_ISL_18408701-18408702, EPI_ISL_18408814, EPI_ISL_18408878, EPI_ISL_18411301, EPI_ISL_18415920, EPI_ISL_18435710, EPI_ISL_18438964, EPI_ISL_18439203, EPI_ISL_18439262-18439263, EPI_ISL_18439368, EPI_ISL_18439436, EPI_ISL_18460639, EPI_ISL_18460726, EPI_ISL_18460826, EPI_ISL_18461023, EPI_ISL_18461030, EPI_ISL_18461109, EPI_ISL_18461117, EPI_ISL_18461139, EPI_ISL_18488790, EPI_ISL_18488878, EPI_ISL_18488904, EPI_ISL_18489119, EPI_ISL_18501908, EPI_ISL_18502007, EPI_ISL_18503617, EPI_ISL_18503624, EPI_ISL_18503755, EPI_ISL_18503952, EPI_ISL_18503974, EPI_ISL_18511170, EPI_ISL_18511222, EPI_ISL_18511248, EPI_ISL_18511257, EPI_ISL_18511484, EPI_ISL_18513699, EPI_ISL_18518361, EPI_ISL_18535616, EPI_ISL_18535618, EPI_ISL_18535641, EPI_ISL_18535643-18535644, EPI_ISL_18535683, EPI_ISL_18539076, EPI_ISL_18539319, EPI_ISL_18539641, EPI_ISL_18539827, EPI_ISL_18540358, EPI_ISL_18544082, EPI_ISL_18547609, EPI_ISL_18547984, EPI_ISL_18550336, EPI_ISL_18552522, EPI_ISL_18559930, EPI_ISL_18564453, EPI_ISL_18564477, EPI_ISL_18564533, EPI_ISL_18564565, EPI_ISL_18564603, EPI_ISL_18564701, EPI_ISL_18564813, EPI_ISL_18564892, EPI_ISL_18564897, EPI_ISL_18565136`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_356f8_c1a890.json?label=id:node_3518019 ![image](https://github.com/cov-lineages/pango-designation/assets/125747944/4396addb-0170-4ef0-b401-e67de407edec)

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Branch 29

Now pulled under HK.3 **Defining Mutations:** **EG.5.1.1** > `C16726T(ORF1b:H1087Y)` > `G9424T, A12472G, G22927T(S:L455F), T23030C(**S:F490P**), G27621T(ORF7a:Q76H)` **Query:** `A12472G, G22927T, G27621T` **Earliest seq:** 2023-08-25 (`EPI_ISL_18245860`, Fujian, China) **Latest seq:** 2023-09-06 (`EPI_ISL_18243501`, Singapore) **Sampled Countries:** Singapore (2), China (1, FJ)
Genomes: `EPI_ISL_18243491, EPI_ISL_18243501, EPI_ISL_18245860`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_4007a_342f0.json?label=id:node_10825409 ![image](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/59683863-45b7-46a7-b315-5bbea3f6b722)

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Branch 30

Now pulled under HK.3 **Defining Mutations:** **EG.5.1.1** > `C4423T` > `G22927T(S:L455F)` **Query:** `C4423T, G13627A, G22927T` **Earliest seq:** 2023-08-12 (`EPI_ISL_18246284`, Sichuan, China) **Latest seq:** 2023-08-25 (`EPI_ISL_18235973`, Chiba, Japan) **Sampled Countries:** China (2, SC), Japan (1)
Genomes: `EPI_ISL_18232772, EPI_ISL_18235973, EPI_ISL_18246284`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_5299_39270.json?f_userOrOld=uploaded%20sample&label=id:node_6954721 ![image](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/195711be-284c-430e-bd45-05e4c2498c26)

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Branch 31

Transferred to main repo _Transferred to cov-lineages/pango-designation#2321_ **Defining Mutations:** **EG.5.1.1** > `C12020T(ORF1a:L3919F), T24496C` > `C2232T(ORF1a:A656V), C21516T, G22927T(S:L455F)` **Query:** `C6541T, C12020T, G22927T, -G6337T, -C8982A, -G18445A, -C28087T` **Earliest seq:** 2023-08-21 (`EPI_ISL_18216560`, Galway, Ireland) **Latest seq:** 2023-09-04 (`EPI_ISL_18254216`, Hubei) **Sampled Countries:** China (1), Ireland (1), UK (1)
Genomes: `EPI_ISL_18216560, EPI_ISL_18244531, EPI_ISL_18254216`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_203fd_1904a0.json?label=id:node_6967052 ![image](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/0d7177d2-65f0-4c89-b10e-f8bfa28477e8)

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Branch 32

Defining Mutations: EG.5.1.1 > T4579A > G2329A, A8443G > G22927T(S:L455F) Query: T4579A, A8443G, G22927T Earliest seq: 2023-08-08 (EPI_ISL_18166186, Sichuan) Latest seq: 2023-08-26 (EPI_ISL_18254268, Hubei) Sampled Countries: China (5)

Genomes: `EPI_ISL_18166186, EPI_ISL_18232746, EPI_ISL_18245861, EPI_ISL_18246325, EPI_ISL_18254268`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_647e_1a2900.json?label=id:node_6956460 image

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Branch 33

Designated as EG.5.1.19 _Designated as **EG.5.1.19** via cov-lineages/pango-designation@0807c23 , after transferred to cov-lineages/pango-designation#2322_ **Defining Mutations:** **EG.5.1** > `T26171A(ORF3a:M260K)` > `A17615G(ORF1b:K1383R), G22927T(S:L455F)` **Query:** `G21718T, G22927T, T26171A` **Earliest seq:** 2023-08-09 (`EPI_ISL_18134871`, Daknam, Belgium) **Latest seq:** 2023-09-03 (`EPI_ISL_18252041`, `SEA` airport, Washington, with travel hisotry: Netherlands) **Sampled Countries:** Belgium (1), France (1), Romania (1), USA (1, ex. Netherlands)
Genomes: `EPI_ISL_18134871, EPI_ISL_18229094, EPI_ISL_18252041, EPI_ISL_18254718`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_107fe_1a6cd0.json?f_userOrOld=uploaded%20sample&label=id:node_6953659 ![image](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/7d34dc4e-6940-4696-8973-a95864566e8a)

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Branch 34, NOT FLip but with S:L455S

Defining Mutations: EG.5.1.3 > G2946A(ORF1a:G894D), T15264C, T22926C(S:L455S) Query: C1889T, C2334T, T22926C, -A4340G, -A10703C Earliest seq: 2023-07-27 (EPI_ISL_18248425, Zagreb, Croatia) Latest seq: 2023-07-31 (EPI_ISL_18248430, Zagreb, Croatia) Sampled Countries: Croatia (4)

Genomes: `EPI_ISL_18248425, EPI_ISL_18248427, EPI_ISL_18248429-18248430`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_1ed45_24dab0.json?f_userOrOld=uploaded%20sample&label=id:node_10831638 image

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Branch 35, side-branch to JJ.1

Transferred to main repo _Transferred to cov-lineages/pango-designation#2354_ **Defining Mutations:** **EG.5.1.4** > `C26111T(ORF3:P240L)` > `C18555T` > `T17268C` > `G22927T(S:L455F)` **Query:** `T17268C, G22927T, C26111T` **Earliest seq:** 2023-08-08 (`EPI_ISL_18132133`, Newfoundland and Labrador) **Latest seq:** 2023-10-23 (`EPI_ISL_18448815`, Ontario, Canada) **Sampled Countries:** Canada (49: ON/22; NL/15; AB/10; BC/1; NS/1), US (7: CA/2; NY/2; AZ/1; UT/1; VA/1), France (1)
Genomes: `EPI_ISL_18132133, EPI_ISL_18256806, EPI_ISL_18258767, EPI_ISL_18258769-18258770, EPI_ISL_18258773, EPI_ISL_18258778, EPI_ISL_18291996, EPI_ISL_18291998, EPI_ISL_18292017, EPI_ISL_18292020-18292022, EPI_ISL_18292026-18292028, EPI_ISL_18333999, EPI_ISL_18344763, EPI_ISL_18357035, EPI_ISL_18372328, EPI_ISL_18372367, EPI_ISL_18383112, EPI_ISL_18383123-18383124, EPI_ISL_18383697, EPI_ISL_18388693, EPI_ISL_18395288, EPI_ISL_18395414, EPI_ISL_18395581, EPI_ISL_18404467, EPI_ISL_18425010, EPI_ISL_18425420, EPI_ISL_18425431, EPI_ISL_18425847, EPI_ISL_18425967, EPI_ISL_18426727, EPI_ISL_18427300, EPI_ISL_18427492, EPI_ISL_18427494, EPI_ISL_18427496, EPI_ISL_18427507, EPI_ISL_18427602, EPI_ISL_18431345, EPI_ISL_18436820, EPI_ISL_18437028, EPI_ISL_18437176, EPI_ISL_18438632, EPI_ISL_18438777, EPI_ISL_18448237, EPI_ISL_18448584-18448585, EPI_ISL_18448588, EPI_ISL_18448699-18448700, EPI_ISL_18448702, EPI_ISL_18448740, EPI_ISL_18448815`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_17f71_c79a0.json?f_userOrOld=uploaded%20sample&label=id:node_3447785 view from EG.5.1.4 polytomy ![sc](https://github.com/cov-lineages/pango-designation/assets/125747944/ebe6d072-41ab-4699-892f-106d43762671) details ![image](https://github.com/cov-lineages/pango-designation/assets/125747944/eedd1bf9-8911-4fee-a970-ca8e9fca6d2f)

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Branch 36

Transferred to main repo: _Transferred to cov-lineages/pango-designation#2399_ **Defining Mutations:** **EG.5.1.1** > `C22388T(S:L276L)` > `G22927T(S:L455F)`, mostly with >> `C1853T` sub-branch **Query:** `C1853T, G13627A, G22927T, -C4780T, -C5031T, -C6627T, -C8982A` (for major `C1853T` sub-branch only) **Earliest seq:** 2023-08-14 (`EPI_ISL_18224087`, Colorado, USA) **Latest seq:** 2023-11-20 (`EPI_ISL_18568071`, `SEA` airport, travel history: France) **Sampled Countries:** France (20), Norway (12), Germany (11), Switzerland (8), USA (6: CO/2; ex. France/2; ex.Germany/2), UK (4: EN), China (3: XJ/2; FJ/1), Denmark (3), Ukraine (3), Canada (2: NB; ON), Finland (2), Spain (2), Belgium (1), Italy (1), Japan (1), Singapore (1)
Genomes: `EPI_ISL_18224087, EPI_ISL_18224096, EPI_ISL_18233134, EPI_ISL_18239749, EPI_ISL_18263561, EPI_ISL_18263571, EPI_ISL_18263574, EPI_ISL_18263576, EPI_ISL_18272601-18272602, EPI_ISL_18280654, EPI_ISL_18280813, EPI_ISL_18290994, EPI_ISL_18291008, EPI_ISL_18291011, EPI_ISL_18308259, EPI_ISL_18313749, EPI_ISL_18326424, EPI_ISL_18326608, EPI_ISL_18327645, EPI_ISL_18337648, EPI_ISL_18337651, EPI_ISL_18338378, EPI_ISL_18373487, EPI_ISL_18373856, EPI_ISL_18380089, EPI_ISL_18380091, EPI_ISL_18388662, EPI_ISL_18388711, EPI_ISL_18411952, EPI_ISL_18412072, EPI_ISL_18421745, EPI_ISL_18421949, EPI_ISL_18428144, EPI_ISL_18428460, EPI_ISL_18428992, EPI_ISL_18433011, EPI_ISL_18441991, EPI_ISL_18451507, EPI_ISL_18464584, EPI_ISL_18464947, EPI_ISL_18468968, EPI_ISL_18476770, EPI_ISL_18485554, EPI_ISL_18486361, EPI_ISL_18486756, EPI_ISL_18493947, EPI_ISL_18494748, EPI_ISL_18495920, EPI_ISL_18496297, EPI_ISL_18496628, EPI_ISL_18496636, EPI_ISL_18496703, EPI_ISL_18499460, EPI_ISL_18505686, EPI_ISL_18505750, EPI_ISL_18519177-18519178, EPI_ISL_18525816, EPI_ISL_18530654, EPI_ISL_18530657-18530658, EPI_ISL_18530757, EPI_ISL_18530938, EPI_ISL_18530967, EPI_ISL_18538000, EPI_ISL_18540615, EPI_ISL_18540768, EPI_ISL_18540871, EPI_ISL_18540915, EPI_ISL_18544152, EPI_ISL_18552615, EPI_ISL_18553571, EPI_ISL_18556840, EPI_ISL_18568060, EPI_ISL_18568071, EPI_ISL_18568733-18568735, EPI_ISL_18570228`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_bd36_c24960.json?label=id:node_3504156 ![image](https://github.com/cov-lineages/pango-designation/assets/125747944/7a99bd61-6514-4aa5-a049-96e0c5fe601a)

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Branch 37

C29095T became homoplastic across the EG.5.1+L455F tree, no longer tracking.

No longer tracking **Defining Mutations:** **EG.5.1.1** > `C29095T` > `G22927T(S:L455F)` **Query:** `G13627A, G22927T, C29095T` **Earliest seq:** 2023-08-28 (`EPI_ISL_18246374`, Sichuan, China) **Latest seq:** 2023-08-31 (`EPI_ISL_18264700`, Texas, USA) **Sampled Countries:** USA (2: TX), China (1: SC)
Genomes: `EPI_ISL_18246374, EPI_ISL_18264482, EPI_ISL_18264700`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_832a_59eef0.json?f_userOrOld=uploaded%20sample&label=id:node_10885350 ![image](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/31d6a883-5850-42ab-ad89-839167a19b8a)

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Branch 38

Defining Mutations: EG.5.1.1 > T24583C > G22927T(S:L455F) Query: C6541T, G22927T, T24583C Earliest seq: 2023-08-03 (EPI_ISL_18166149, Sichuan) Latest seq: 2023-08-26 (EPI_ISL_18268462 Shanghai) Sampled Countries: China (3: SC/2; SH/1)

Genomes: `EPI_ISL_18166149, EPI_ISL_18232692, EPI_ISL_18268462`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_f9a8_5a58c0.json?f_userOrOld=uploaded%20sample&label=id:node_10885352 image

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Branch 39

Defining Mutations: HK.1 (EG.5.1.1.1) > G22927T(S:L455F) Query: C6541T, G13627A, G22332T, G22927T + C6541T, G13627A, G22332A, G22927T Earliest seq: 2023-08-09 (EPI_ISL_18146086, Hubei) Latest seq: 2023-08-28 (EPI_ISL_18254226, Hubei) Sampled Countries: China (7)

Genomes: `EPI_ISL_18146086, EPI_ISL_18146100, EPI_ISL_18254226-18254227, EPI_ISL_18268425, EPI_ISL_18138066, EPI_ISL_18254258`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_1cca6_5afd40.json?f_userOrOld=uploaded%20sample&label=id:node_6971951 image

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Branch 40

Defining Mutations: EG.5.1.1 > C10615T > G22927T(S:L455F) > C1426T, G25691T(ORF3a:G100V) Query: C1426T, C10615T, G22927T Earliest seq: 2023-08-08 (EPI_ISL_18138249, Shanghai) Latest seq: 2023-08-25 (EPI_ISL_18268441, Shanghai) Sampled Countries: China (4: SH)

Genomes: `EPI_ISL_18138249, EPI_ISL_18208636, EPI_ISL_18268441, EPI_ISL_18268482`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_20010_5b23f0.json?f_userOrOld=uploaded%20sample&label=id:node_6983332 image

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Branch 41

Defining Mutations: EG.5.1.1 > C24023T > C2485T, G15025A(ORF1b:A520T), G22927C(S:L455F) Query: C6541T, G22927C, C24023T Earliest seq: 2023-08-14 (EPI_ISL_18239206, South Korea) Latest seq: 2023-08-24 (EPI_ISL_18254859, South Korea) Sampled Countries: South Korea (3)

Genomes: `EPI_ISL_18239206, EPI_ISL_18254859, EPI_ISL_18271926`

UShER: https://nextstrain.org/fetch/genome-test.gi.ucsc.edu/trash/ct/subtreeAuspice1_genome_test_26c10_8252f0.json?f_userOrOld=uploaded%20sample&label=id:node_10885690 image

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Branch 42

Transferred to main repo _Transferred to cov-lineages/pango-designation#2355_ **Defining Mutations:** **EG.5.1.1** > `C22687T` > `G22927T(S:L455F)` **Query:** `C6541T, C22687T, G22927T, -T2145C, -A16482C, -C21191T` **Earliest seq:** 2023-08-14 (`EPI_ISL_18279039`, Singapore, travel history: China) **Latest seq:** 2023-09-01 (`EPI_ISL_18278560`, Alberta, Canada) **Sampled Countries:** China (21: GS/4; HB/3; XJ/3; AH/2; SC/2; SX/2; FJ/1; HK/1; QH/1; SH/1; YN/1), Australia (12: NSW/5; SA/4; QLD/1; TA/1; VIC/1), South Korea (8), Canada (6: AB), USA (3: AZ/1; ex. Australia/2), Singapore (2: Local/1; ex. China/1), Japan (1)
Genomes: `EPI_ISL_18146046, EPI_ISL_18213336, EPI_ISL_18217223, EPI_ISL_18278558, EPI_ISL_18278560, EPI_ISL_18279039, EPI_ISL_18289881, EPI_ISL_18293677, EPI_ISL_18293698, EPI_ISL_18311984, EPI_ISL_18312274, EPI_ISL_18312380, EPI_ISL_18313209, EPI_ISL_18327623, EPI_ISL_18327666, EPI_ISL_18346251, EPI_ISL_18346325, EPI_ISL_18347707, EPI_ISL_18350428, EPI_ISL_18360224, EPI_ISL_18361336, EPI_ISL_18361999, EPI_ISL_18365676, EPI_ISL_18365684, EPI_ISL_18365890, EPI_ISL_18366374, EPI_ISL_18382081, EPI_ISL_18382589, EPI_ISL_18382710, EPI_ISL_18384562, EPI_ISL_18388967, EPI_ISL_18389014, EPI_ISL_18389016, EPI_ISL_18390872, EPI_ISL_18396110, EPI_ISL_18401894, EPI_ISL_18401903, EPI_ISL_18401983, EPI_ISL_18404054, EPI_ISL_18408752, EPI_ISL_18411422, EPI_ISL_18411665, EPI_ISL_18411717, EPI_ISL_18411753, EPI_ISL_18425089, EPI_ISL_18427884, EPI_ISL_18435235, EPI_ISL_18438336, EPI_ISL_18438338, EPI_ISL_18438347, EPI_ISL_18439100, EPI_ISL_18447517, EPI_ISL_18447771`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_27ac0_cb920.json?f_userOrOld=uploaded%20sample&label=id:node_3487678 ![image](https://github.com/cov-lineages/pango-designation/assets/125747944/f169acff-e2c6-411a-9a48-74e4c93d0080)

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Branch 43

now seems to be pulled under HK.3, no longer tracking

Pulled under HK.3 **Defining Mutations:** **EG.5.1.1** > `C19524T` > `G22927T(S:L455F), A28254Δ` **Query:** `G13627A, C19524T, G22927T, -C5031T` **Earliest seq:** 2023-08-19 (`EPI_ISL_18246322`, Sichuan) **Latest seq:** 2023-09-07 (`EPI_ISL_18277156`, Fujian) **Sampled Countries:** China (3: FJ; HL; SC)
Genomes: `EPI_ISL_18246049, EPI_ISL_18246322, EPI_ISL_18277156`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice2_genome_22869_97c570.json?f_userOrOld=uploaded%20sample&label=id:node_6973155 ![image](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/0d31d03f-4db8-40d2-83b4-2251d6576905)

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Branch 44, "FLippeR"

Transferred to main Repo _Transferred to cov-lineages/pango-designation#2296_ **Defining Mutations:** **EG.5.1** > `A22106C(S:K182Q)` > `C22995G(S:T478R), A25664G(ORF3a:Y91C)` > `C1613T(ORF1a:L450F), G20995T(ORF1b:G2510C), G22927C(S:L455F), C25803T, A27388C` **Query:** `A22106C, G22927C, C22995G` **Earliest seq:** 2023-08-14 (`EPI_ISL_18160020`, Denmark, or, `EPI_ISL_18278998`, Singapore) **Latest seq:** 2023-09-04 (`EPI_ISL_18243476`, Singapore) **Sampled Countries:** Singapore (2), Denmark (1)
Genomes: `EPI_ISL_18160020, EPI_ISL_18243476, EPI_ISL_18278998`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_13bc1_9943e0.json?f_userOrOld=uploaded%20sample&label=id:node_6964487 ![image](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/fcb5c1b8-c4ad-45ad-9de2-4ea85fb99d11)

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Branch 45

Transferred to main Repo _Transferred to cov-lineages/pango-designation#2308_ **Defining Mutations:** `EG.5.1.1` > `C13968A, A15885G` > `T11449C` > `C10369T, G22927T(S:L455F), C29077T, G29140T(N:Q289H)` **Query:** `T11449C, C13968A, G22927T` **Earliest seq:** 2023-09-06 (`EPI_ISL_18273676`, England) **Latest seq:** 2023-09-07 (`EPI_ISL_18284457`, `IAD` Airport, DC, travel history: Italy) **Sampled Countries:** UK (2: EN) USA (2, ex. Italy)
Genomes: `EPI_ISL_18273676, EPI_ISL_18281373, EPI_ISL_18284072, EPI_ISL_18284457`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_2d447_b0ad70.json?f_userOrOld=uploaded%20sample&label=id:node_6985113 ![image](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/27b21ec6-1008-464f-af1b-63360739cd44)

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Branch 46 with a sub-lineage of S:L452Q, designated as HK.22

parental lineage of HK.22 _Branch 46 + S:L452Q designated as HK.22 via cov-lineages/pango-designation@2d3115a_ **Defining Mutations:** `EG.5.1.1` > `G25407T(ORF3a:M5I)` > `C2662T` > `G22927T(S:L455F)` **Query:** `C2662T, G22927T, G25407T` **Earliest seq:** 2023-08-06 (`EPI_ISL_18114750`, Shanghai) **Latest seq:** 2023-09-06 (`EPI_ISL_18284970`, Shanghai) **Sampled Countries:** China (4: SH)
Genomes: `EPI_ISL_18114750, EPI_ISL_18232688, EPI_ISL_18284888, EPI_ISL_18284970`
**UShER:** https://nextstrain.org/fetch/genome-test.gi.ucsc.edu/trash/ct/subtreeAuspice1_genome_test_38b1_b0cc80.json?f_userOrOld=uploaded%20sample ![image](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/7e73e4f7-3aad-4d6b-b19e-33927c86bc31)

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Branch 47

Defining Mutations: EG.5.1.1 > C7600T > G22927T(S:L455F) > T10216C Query: C7600T, T10216C, G22927T Earliest seq: 2023-08-22 (EPI_ISL_18246331, Sichuan) Latest seq: 2023-09-04 (EPI_ISL_18284731, Hubei) Sampled Countries: China (5)

Genomes: `EPI_ISL_18246331, EPI_ISL_18284731, EPI_ISL_18284776, EPI_ISL_18284783, EPI_ISL_18284960`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_3ad47_b108e0.json?f_userOrOld=uploaded%20sample&label=id:node_10899080 image

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Branch 48

Transferred to main repo Transferred to cov-lineages/pango-designation#2360 ⚠️ Please check potential misplacement. **Defining Mutations:** `EG.5.1.1` > `A23539T(S:S659S)` > `G22927T(S:L455F)` **Query:** `G13627A, G22927T, A23539T, -T899C, -C2662T, -C10015T, -T12865C, -G22332A` **Earliest seq:** 2023-08-16 (`EPI_ISL_18141107`, Singapore) **Latest seq:** 2023-10-22 (`EPI_ISL_18440472`, Netherlands) **Sampled Countries:** Australia (32: NSW/14; SA/8; QLD/4; VIC/3; TAS/1; WA/1), China (10: SC/4; HB/2; SH/2; FJ/1; XJ/1), New Zealand (9), Singapore (4), Netherlands (3), USA (2), France (1)
Genomes: `EPI_ISL_18141107, EPI_ISL_18246326, EPI_ISL_18278997, EPI_ISL_18279955, EPI_ISL_18292475, EPI_ISL_18300669, EPI_ISL_18307132, EPI_ISL_18320781, EPI_ISL_18324527, EPI_ISL_18324537, EPI_ISL_18324951, EPI_ISL_18346779, EPI_ISL_18346805, EPI_ISL_18346818, EPI_ISL_18347516, EPI_ISL_18347639, EPI_ISL_18347703-18347704, EPI_ISL_18347710, EPI_ISL_18352381, EPI_ISL_18355046, EPI_ISL_18360352, EPI_ISL_18365605, EPI_ISL_18365672, EPI_ISL_18365874, EPI_ISL_18376439, EPI_ISL_18388898, EPI_ISL_18396188, EPI_ISL_18411419, EPI_ISL_18411467, EPI_ISL_18411551, EPI_ISL_18411564, EPI_ISL_18411674, EPI_ISL_18411695, EPI_ISL_18423924, EPI_ISL_18427871, EPI_ISL_18428112, EPI_ISL_18435043, EPI_ISL_18438373, EPI_ISL_18440466, EPI_ISL_18440472, EPI_ISL_18440476, EPI_ISL_18442757, EPI_ISL_18442774, EPI_ISL_18447732, EPI_ISL_18454463, EPI_ISL_18454489, EPI_ISL_18457508, EPI_ISL_18459380, EPI_ISL_18459604, EPI_ISL_18459721, EPI_ISL_18459855, EPI_ISL_18459912, EPI_ISL_18459940, EPI_ISL_18461585, EPI_ISL_18461782, EPI_ISL_18461792, EPI_ISL_18461895, EPI_ISL_18461916, EPI_ISL_18461956, EPI_ISL_18464115`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_2e62_757100.json?label=id:node_3495970 ![image](https://github.com/cov-lineages/pango-designation/assets/125747944/0c8ae009-2418-4dc0-91ad-5509025355a1) **Trivia:** - Please check potential misplacement: it seems only one seqs preceeding `S:L455F` branch, being `Sichuan/BZCDC-043/2023|EPI_ISL_17988879|2023-06-12`; however the mutations on the follow-up sub-branch `G571A, C2263A, A28959G` are "unique" to the EG.5.1.1* tree which may support it be a different lineage than `HK.3`.

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Branch 49

Now at least 3 smaller clusters seems pulled together a S:L455F branch right under EG.5.1.4 polytomy, hard to catch by proper query now.

Query failed **Defining Mutations:** `EG.5.1.4` > `C8407T, C11008A(ORF1a:H3581Q), G22927T(S:L455F), G25538T(ORF3a:G49V), C25572T` **Query:** `A18492G, A21137G, G22927T, -C6120T, -G13627A, -C26111T` **Earliest seq:** 2023-08-21 (`EPI_ISL_18284756`, Hubei) **Latest seq:** 2023-09-03 (`EPI_ISL_18284729`, Hubei) **Sampled Countries:** China (3)
Genomes: `EPI_ISL_18284721, EPI_ISL_18284729, EPI_ISL_18284756`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_176c9_b20830.json?f_userOrOld=uploaded%20sample&label=id:node_10899080 ![image](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/7d19e39f-fde3-4a4e-a586-d1ab877b9ca1)

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Branch 50

Defining Mutations: EG.5.1.1 > G28904T(N:A211S) > G22927T(S:L455F), G27261T, C28744T Query: C6541T, G22927T, G28904T Earliest seq: 2023-08-30 (EPI_ISL_18268410, Shanghai) Latest seq: 2023-09-03 (EPI_ISL_18254255, Hubei) Sampled Countries: China (5)

Genomes: `EPI_ISL_18254255, EPI_ISL_18268410, EPI_ISL_18289864, EPI_ISL_18289874, EPI_ISL_18289889`

UShER: https://nextstrain.org/fetch/genome-test.gi.ucsc.edu/trash/ct/subtreeAuspice1_genome_test_523af_c118f0.json?f_userOrOld=uploaded%20sample&label=id:node_6955662 image

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Branch 51

Transferred to main repo _transferred to cov-lineages/pango-designation#2365_ **Defining Mutations:** EG.5.1.1 > `C10015T` > `G22927T(S:L455F)` **Query:** `C6541T, C10015T, G22927T, -C4586T` **Earliest seq:** 2023-07-31 (`EPI_ISL_18246153`, Heilongjiang) **Latest seq:** 2023-10-31 (`EPI_ISL_18473950`, Spain) **Sampled Countries:** China (20: SH/6; HL/5; LN/3; AH/1; BJ/1; GS/1; HB/1; SC/1; SX/1; XJ/1), Canada (10: ON/9; NS/1), Australia (5: NSW/4; QLD/1), Singapore (5), Spain (5), Croatia (4), USA (3: NY/2; HI/1), Denmark (2), Japan (2), Belgium (1), France (1), Italy (1), Switzerland (1)
Genomes: `EPI_ISL_18246153, EPI_ISL_18268220, EPI_ISL_18284760, EPI_ISL_18289806, EPI_ISL_18304238, EPI_ISL_18307185, EPI_ISL_18309728, EPI_ISL_18320892, EPI_ISL_18333882, EPI_ISL_18354453, EPI_ISL_18356455, EPI_ISL_18362071, EPI_ISL_18382338, EPI_ISL_18384364, EPI_ISL_18388937, EPI_ISL_18388943, EPI_ISL_18392626, EPI_ISL_18392641, EPI_ISL_18395616, EPI_ISL_18398918, EPI_ISL_18401900, EPI_ISL_18422863, EPI_ISL_18425076, EPI_ISL_18433557, EPI_ISL_18438020, EPI_ISL_18438314, EPI_ISL_18438838, EPI_ISL_18445101, EPI_ISL_18445501, EPI_ISL_18446144, EPI_ISL_18448315, EPI_ISL_18448907-18448908, EPI_ISL_18448913-18448914, EPI_ISL_18461557, EPI_ISL_18461608, EPI_ISL_18461684, EPI_ISL_18461722, EPI_ISL_18464481, EPI_ISL_18464633, EPI_ISL_18465094, EPI_ISL_18465113, EPI_ISL_18465171, EPI_ISL_18465395, EPI_ISL_18465403, EPI_ISL_18466062, EPI_ISL_18466326-18466327, EPI_ISL_18466420, EPI_ISL_18466590, EPI_ISL_18470572, EPI_ISL_18471609, EPI_ISL_18471611, EPI_ISL_18473319, EPI_ISL_18473421, EPI_ISL_18473950, EPI_ISL_18475156, EPI_ISL_18475172, EPI_ISL_18475181, EPI_ISL_18475190, EPI_ISL_18475453`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_25e8a_b47430.json?label=id:node_3494261 ![image](https://github.com/cov-lineages/pango-designation/assets/125747944/8b3d4bc0-5d1a-439a-8ad5-e4f1bf14da67)

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Branch 52, now pulled under HK.3.2

Now part of HK.3.2 **Defining Mutations:** `EG.5.1.1` > `C26029A(ORF3a:Q213K)` > `G22927T(S:L455F)` **Query:** `C6541T, G22927T, C26029A, -G24944T, -A26901G` **Earliest seq:** 2023-08-16 (`EPI_ISL_18146001`, Hubei, China) **Latest seq:** 2023-09-20 (`EPI_ISL_18340137`, Illinois, USA) **Sampled Countries:** China (5: HB/3; SC; YN) USA (3: IL; NY; WA), Oman (1)
Genomes: `EPI_ISL_18146001, EPI_ISL_18246341, EPI_ISL_18290983, EPI_ISL_18292780, EPI_ISL_18313122, EPI_ISL_18322921, EPI_ISL_18330205, EPI_ISL_18333620, EPI_ISL_18340137`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_36145_d6af90.json?f_userOrOld=uploaded%20sample&label=id:node_3648445 ![image](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/4c9dae65-c4c4-4533-b700-c93e312c3267)

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Branch 53

Transferred to main repo _transferred to cov-lineages/pango-designation#2366_ **Defining Mutations:** `EG.5.1.1` > `C27741T` > `C1912T, C1968T(ORF1a:T568I), T7111C, A20010T(ORF1b:Q2181H), T20592C, G22927C(S:L455F), C28093T(ORF8:S67F)` **Query:** `C6541T, G22927C, C27741T` **Earliest seq:** 2023-08-28 (`EPI_ISL_18345224`, Cork, Ireland) **Latest seq:** 2023-10-23 (`EPI_ISL_18471085`, Connecticut, USA) **Sampled Countries:** UK (17: EN/16; WS/1), USA (14: NY/3; NJ/2; TX/2; UT/2; AZ/1; CA/1; CT/1; MN/1; OH/1), Ireland (9), Canada (4: ON/3; BC/1), Denmark (2), France (2), China (2: SC/1; ex. Spain/1), Belgium (1), South Korea (1), Netherlands (1)
Genomes: `EPI_ISL_18250090, EPI_ISL_18284832, EPI_ISL_18290882, EPI_ISL_18290915, EPI_ISL_18308328, EPI_ISL_18308339, EPI_ISL_18315360, EPI_ISL_18321565, EPI_ISL_18332107, EPI_ISL_18332505, EPI_ISL_18345224, EPI_ISL_18354139, EPI_ISL_18362839, EPI_ISL_18362880-18362881, EPI_ISL_18362951, EPI_ISL_18375850, EPI_ISL_18377642, EPI_ISL_18385706, EPI_ISL_18385720, EPI_ISL_18385728, EPI_ISL_18385734, EPI_ISL_18388184, EPI_ISL_18388760-18388761, EPI_ISL_18389241, EPI_ISL_18398829, EPI_ISL_18399988, EPI_ISL_18400877, EPI_ISL_18403587, EPI_ISL_18403998, EPI_ISL_18407932, EPI_ISL_18416430, EPI_ISL_18416450, EPI_ISL_18420893, EPI_ISL_18420911, EPI_ISL_18422267, EPI_ISL_18427521, EPI_ISL_18429173, EPI_ISL_18429518, EPI_ISL_18430506, EPI_ISL_18433870, EPI_ISL_18438642, EPI_ISL_18442008, EPI_ISL_18443922, EPI_ISL_18448322, EPI_ISL_18452961, EPI_ISL_18456955, EPI_ISL_18467439, EPI_ISL_18467445, EPI_ISL_18467479, EPI_ISL_18471085, EPI_ISL_18475314`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_2eee6_b4b600.json?label=id:node_3484946 ![image](https://github.com/cov-lineages/pango-designation/assets/125747944/7a31b77c-042f-44ee-b8fc-0e102b4632b7)

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Branch 54:

Defining Mutations: EG.5.1.1 > G24368T(S:D936Y) > G22927T(S:L455F) Query: G13627A, G22927T, G24368T, -G3567A, -C4012T, -C5031T, -T13234C, -C17976T Earliest seq: 2023-09-11 (EPI_ISL_18312655, South Korea) Latest seq: 2023-11-24 (EPI_ISL_18567787, Veneto, Italy) Sampled Countries: Finland (15), South Korea (15), USA (9: NY/7; UT/2), Italy (2), Singapore (2), Australia (1: QLD), Germany (1)

Genomes: `EPI_ISL_18312655, EPI_ISL_18343453, EPI_ISL_18347772, EPI_ISL_18347778, EPI_ISL_18347783, EPI_ISL_18347794, EPI_ISL_18347862, EPI_ISL_18354114, EPI_ISL_18394470, EPI_ISL_18408694, EPI_ISL_18411981, EPI_ISL_18411986, EPI_ISL_18412002, EPI_ISL_18412024, EPI_ISL_18412052, EPI_ISL_18412131, EPI_ISL_18439287, EPI_ISL_18439475, EPI_ISL_18445086, EPI_ISL_18445161, EPI_ISL_18449963, EPI_ISL_18458264, EPI_ISL_18458329, EPI_ISL_18460569, EPI_ISL_18502041, EPI_ISL_18503589, EPI_ISL_18511443, EPI_ISL_18533418, EPI_ISL_18533465, EPI_ISL_18533560, EPI_ISL_18533727, EPI_ISL_18534149, EPI_ISL_18539237, EPI_ISL_18539435, EPI_ISL_18539679, EPI_ISL_18539701, EPI_ISL_18539776, EPI_ISL_18541395, EPI_ISL_18541532, EPI_ISL_18544389, EPI_ISL_18547900, EPI_ISL_18554927, EPI_ISL_18555070, EPI_ISL_18564736, EPI_ISL_18567787`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_28b3b_c482d0.json?label=id:node_3526683 image

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Branch 55

Designated with 2 levesl: HK.24 and HK.24.1 Designated as [HK.24](https://github.com/cov-lineages/pango-designation/commit/211018361b201eda9d492fdb25dc7fbcad217804) and [HK.24.1](https://github.com/cov-lineages/pango-designation/commit/77596389b8d6291450be40a5b8a7c1701c9e5309) **Defining Mutations:** `EG.5.1.1` > `G23522A(S:E654K)` > `G22927T(S:L455F)` **Query:** `C6541T, G22927T, G23522A` **Earliest seq:** 2023-08-26 (`EPI_ISL_18284795`, Shanghai, China) **Latest seq:** 2023-09-24 (`EPI_ISL_18346833`, South Australia, Australia) **Sampled Countries:** Australia (6: QLD/2; WA/2; NSW; SA), China (3: SH), New Zealand (1)
Genomes: `EPI_ISL_18284795, EPI_ISL_18284982, EPI_ISL_18324864, EPI_ISL_18346833, EPI_ISL_18347678, EPI_ISL_18352382, EPI_ISL_18352389, EPI_ISL_18352581, EPI_ISL_18354460, EPI_ISL_18360253`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_222ea_2ee540.json?f_userOrOld=uploaded%20sample&label=id:node_3445909 ![image](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/c10096c2-de1d-4afe-b950-45cb5ba050e2)

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Branch 56

Defining Mutations: EG.5.1.1 > A22582G(S:E340) > G22927T(S:L455F) Query: G13627A, A22582G, G22927T, -G3153A Earliest seq: 2023-08-30 (EPI_ISL_18303241, Portugal) Latest seq: 2023-09-25 (EPI_ISL_18355054, Singapore) Sampled Countries: Portugal (2), Singapore (2), China (1: QH), UK (1: EN)

Genomes: `EPI_ISL_18268272, EPI_ISL_18274005, EPI_ISL_18303241, EPI_ISL_18303432, EPI_ISL_18307200, EPI_ISL_18355054`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_61cc_2ffcf0.json?label=id:node_3450332 image

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Branch 57

Defining Mutations: EG.5.1.1 > A22582G(S:E340) > G3153A(ORF1a:G963D) > G22927T(S:L455F) Query: G3153A, A22582G, G22927T Earliest seq: 2023-08-14 (EPI_ISL_18146012, Hubei, China) Latest seq: 2023-09-26 (EPI_ISL_18350530, Basque, Spain) Sampled Countries: China (4: FJ; HB; HK; SH), Japan (2), South Korea (2), USA (2: ex. Greece), Spain (1)

Genomes: `EPI_ISL_18146012, EPI_ISL_18277143, EPI_ISL_18293356, EPI_ISL_18306220, EPI_ISL_18306632, EPI_ISL_18306769, EPI_ISL_18309318, EPI_ISL_18309324, EPI_ISL_18350530, EPI_ISL_18354289, EPI_ISL_18360277`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_61cc_2ffcf0.json?label=id:node_3450116 image

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Branch 58, formerly singlet, thanks to @aviczhl2 for updated info

Defining Mutations: EG.5.1 > T26972C > C28909T > C2106A(ORF1a:T614N), G28423T(ORF9b:R47L) > A21759G(S:H66R), G22927T(S:L455F), G24004A(S:K814), C25572T Query: A18492G, G22927T, C28909T Earliest seq: 2023-08-21 (EPI_ISL_18271704, S. Korea) Latest seq: 2023-09-19 (EPI_ISL_18355498, S. Korea) Sampled Countries: South Korea (6)

Genomes: `EPI_ISL_18271704, EPI_ISL_18354058, EPI_ISL_18354072, EPI_ISL_18354264, EPI_ISL_18355236, EPI_ISL_18355498`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_26418_30eca0.json?label=id:node_3429102 image

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Branch 59: ⚠️ careful with potential dropout/misplacement.

Defining Mutations: EG.5.1.1 > G20134T(ORF1b:V2223L) > G22927T(S:L455F) Query: G13627A, G20134T, G22927T Earliest seq: 2023-09-04 (EPI_ISL_18300595, New South Wales) Latest seq: 2023-10-03 (EPI_ISL_18361370, NSW) Sampled Countries: Australia (17: NSW/16; QLD)

Genomes: `EPI_ISL_18300595, EPI_ISL_18300598, EPI_ISL_18300624, EPI_ISL_18300634, EPI_ISL_18300676-18300679, EPI_ISL_18300732, EPI_ISL_18300745-18300746, EPI_ISL_18300790, EPI_ISL_18324806, EPI_ISL_18352578, EPI_ISL_18361360-18361361, EPI_ISL_18361370`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_4fa9_6ad320.json?label=id:node_3451611 image

Trivia:

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Branch 60: Formerly Y144F artifact branch now masked

Defining Mutations: EG.5.1.1 > T16545G > C1471T, G22927T(S:L455F) Query: G13627A, T16545G, G22927T Earliest seq: 2023-08-25 (EPI_ISL_18228384, Finland) Latest seq: 2023-10-04 (EPI_ISL_18362018, Singapore) Sampled Countries: Singapore (7), China (3: SH/2; HB), Finland (1)

Genomes: `EPI_ISL_18228384, EPI_ISL_18243407, EPI_ISL_18254180, EPI_ISL_18306951, EPI_ISL_18320869, EPI_ISL_18360325, EPI_ISL_18361717, EPI_ISL_18361964, EPI_ISL_18362005, EPI_ISL_18362018, EPI_ISL_18362077`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_108b5_6b1720.json?f_userOrOld=uploaded%20sample&label=id:node_3457632 image

Trivia:

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Branch 61

⚠️ Please check potential reversion/artifact/misplacement, C26681T is quite homoplastic in HK.3*, no longer tracking

potential HK.3 dropout **Defining Mutations:** `EG.5.1.1` > `C26681T` > `T5327C, C5986T, T22032C(S:F157S), G22927T(S:L455F)` **Query:** `T22032C, G22927T, C26681T` **Earliest seq:** 2023-09-03 (`EPI_ISL_18376557`, Sichuan) **Latest seq:** 2023-09-11 (`EPI_ISL_18376558`, Sichuan, _OR_ `EPI_ISL_18277141`, Fujian) **Sampled Countries:** China (5: SC/4; FJ)
Genomes: `EPI_ISL_18277141, EPI_ISL_18376556-18376559`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice14_genome_f688_7a5e50.json?label=id:node_3447615 ![image](https://github.com/sars-cov-2-variants/lineage-proposals/assets/125747944/54293b2a-569f-46a5-9652-15875f493154)

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Branch 62: With a sub-branch of FLippeR

Defining Mutations: EG.5.1.1 > C18559T(ORF1b:L1698F) > T15927C, G22927T(S:L455F) Query: C6541T, C18559T, G22927T Earliest seq: 2023-08-31 (EPI_ISL_18285056, Sichuan, China) Latest seq: 2023-09-20 (EPI_ISL_18382134, South Korea) Sampled Countries: China (4: SC/2; SH/2), South Korea (1)

Genomes: `EPI_ISL_18285056-18285057, EPI_ISL_18320863, EPI_ISL_18360320, EPI_ISL_18382134`

UShER: https://nextstrain.org/fetch/genome-test.gi.ucsc.edu/trash/ct/subtreeAuspice1_genome_test_24de1_9159b0.json?label=id:node_3445922 image

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Branch 63

⚠️ Please check potential misplacement

Transferred to main repo _Transferred to cov-lineages/pango-designation#2400_ **Defining Mutations:** `EG.5.1.1` > `T14034C` > `T6556C` > `G22927T(S:L455F)` **Query:** `T6556C, T14034C, G22927T` **Earliest seq:** 2023-08-28 (`EPI_ISL_18236022`, Chiba, Japan) **Latest seq:** 2023-11-16 (`EPI_ISL_18568493`, Ardèche, France) **Sampled Countries:** South Korea (66), Japan (11), France (7), China (2: Taiwan), Singapore (2), Germany (1), Ireland (1), UK (1: England)
Genomes: `EPI_ISL_18236022, EPI_ISL_18307101, EPI_ISL_18307183, EPI_ISL_18312967, EPI_ISL_18354179, EPI_ISL_18354386, EPI_ISL_18371409, EPI_ISL_18371437, EPI_ISL_18376385, EPI_ISL_18381917, EPI_ISL_18382077, EPI_ISL_18382170, EPI_ISL_18382306, EPI_ISL_18382313, EPI_ISL_18384744, EPI_ISL_18390972, EPI_ISL_18408733, EPI_ISL_18408999, EPI_ISL_18409046, EPI_ISL_18409110, EPI_ISL_18418096, EPI_ISL_18418099, EPI_ISL_18420466, EPI_ISL_18423347, EPI_ISL_18438958, EPI_ISL_18439063, EPI_ISL_18439157, EPI_ISL_18439221, EPI_ISL_18439371, EPI_ISL_18460530, EPI_ISL_18460541, EPI_ISL_18460589, EPI_ISL_18460966, EPI_ISL_18460978, EPI_ISL_18461033, EPI_ISL_18461094, EPI_ISL_18461485, EPI_ISL_18465933, EPI_ISL_18468665, EPI_ISL_18486472, EPI_ISL_18491815, EPI_ISL_18496187, EPI_ISL_18501996, EPI_ISL_18502046, EPI_ISL_18502053, EPI_ISL_18502059, EPI_ISL_18503696, EPI_ISL_18503846, EPI_ISL_18510036, EPI_ISL_18510038, EPI_ISL_18511034, EPI_ISL_18511095, EPI_ISL_18511104, EPI_ISL_18511108, EPI_ISL_18511172, EPI_ISL_18511241, EPI_ISL_18511380, EPI_ISL_18511416, EPI_ISL_18511461, EPI_ISL_18511469, EPI_ISL_18511480, EPI_ISL_18511508, EPI_ISL_18511521, EPI_ISL_18511532, EPI_ISL_18511557, EPI_ISL_18511580, EPI_ISL_18511661, EPI_ISL_18511670, EPI_ISL_18535606, EPI_ISL_18535663, EPI_ISL_18535697, EPI_ISL_18539307, EPI_ISL_18539369, EPI_ISL_18539422, EPI_ISL_18539428, EPI_ISL_18539447, EPI_ISL_18539483, EPI_ISL_18539547, EPI_ISL_18545014, EPI_ISL_18564392, EPI_ISL_18564436, EPI_ISL_18564462-18564463, EPI_ISL_18564564, EPI_ISL_18564669, EPI_ISL_18564752, EPI_ISL_18564926, EPI_ISL_18565014, EPI_ISL_18565195, EPI_ISL_18568493, EPI_ISL_18570360`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_21541_c2c6a0.json?label=id:node_3526922 ![image](https://github.com/cov-lineages/pango-designation/assets/125747944/5b90b3a7-63c8-46ce-9548-ea7813bc5bf0) **Trivia:** - Only 3 seqs preceeding this branch without `S:L455F`: `France/GES-IPP16513/2023|EPI_ISL_18109814|2023-07-241, `Australia/QLD0x0138F8/2023|EPI_ISL_18324553|2023-09-12`, and `SouthKorea/KDCA_270758/2023|EPI_ISL_18503707|2023-10-12` ⚠️ so please check potential dropout/misplacement.

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Branch 64

Defining Mutations: EG.5.1.6 > C28236T(ORF8:R115C) > C16092T > C10969T > G526T(ORF1a:E87D) > C23380T(S:N606) > A12485G(ORF1a:I4074V) > T877C, A15270T(ORF1b:E601D), G22927T(S:L455F) Query: G526T, A15270T, G22927T Earliest seq: 2023-10-08 (EPI_ISL_18404805, New Brunswick, Canada) Latest seq: 2023-10-15 (EPI_ISL_18427247, Ontario, Canada) Sampled Countries: Canada (4: ON/3; NB)

Genomes: `EPI_ISL_18404805, EPI_ISL_18426514, EPI_ISL_18426586, EPI_ISL_18427247`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_21e1_9e19a0.json?label=id:node_3437762 image

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Branch 65

Defining Mutations: EG.5.1 > A3210G(ORF1a:D982G) > G26888A > G26227T(_TRS of Envelope_), C28720T(_TRS of ORF9c_) > T8909C> G22927T(S:L455F) Query: A3210G, T8909C, G22927T Earliest seq: 2023-09-07 (EPI_ISL_18419405, Sicily, Italy) Latest seq: 2023-09-18 (EPI_ISL_18329969, Denmark) Sampled Countries: Italy (13), Denmark (1)

Genomes: `EPI_ISL_18258788-18258789, EPI_ISL_18329969, EPI_ISL_18419405, EPI_ISL_18419417, EPI_ISL_18419442, EPI_ISL_18419446, EPI_ISL_18419449, EPI_ISL_18419451, EPI_ISL_18419459, EPI_ISL_18419472, EPI_ISL_18419474, EPI_ISL_18419478, EPI_ISL_18419484`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_3b9a_9e23b0.json?label=id:node_3428133 image

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Branch 66

Defining Mutations: EG.5.1.1 > C11779T > C12525T(ORF1a:T4087I) > G22927T(S:L455F) Query: C11779T, C12525T, G22927T Earliest seq: 2023-08-27 (EPI_ISL_18360191, England) Latest seq: 2023-10-09 (EPI_ISL_18421921, Belgium) Sampled Countries: South Korea (4), Canada (3: BC), Belgium (1), China (1: SH), New Zealand (1), Singapore (1), UK (1: EN)

Genomes: `EPI_ISL_18227053, EPI_ISL_18292157, EPI_ISL_18355538, EPI_ISL_18360191, EPI_ISL_18381927, EPI_ISL_18382266, EPI_ISL_18397328, EPI_ISL_18408737, EPI_ISL_18421921, EPI_ISL_18430928, EPI_ISL_18431490, EPI_ISL_18431860`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_34aaf_9ea630.json?label=id:node_3493682 image

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Branch 67

Defining Mutations: EG.5.1.1 > C25613T(ORF3a:S74F) > G22927C(S:L455F) Query: C6541T, G22927C, C25613T, -C26681T, -G26840A Earliest seq: 2023-10-12 (EPI_ISL_18428810, Singapore) Latest seq: 2023-10-16 (EPI_ISL_18428772, Singapore) Sampled Countries: Singapore (5)

Genomes: `EPI_ISL_18428766, EPI_ISL_18428769, EPI_ISL_18428771-18428772, EPI_ISL_18428810`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_41fd2_a0fe0.json?label=id:node_3487648 image

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Branch 68

Defining Mutations: EG.5.1.1 > G16710A > G22927T(S:L455F) Query: G16710A, G13627A, G22927T, -G28904T Earliest seq: 2023-09-25 (EPI_ISL_18355035, Singapore) Latest seq: 2023-10-18 (EPI_ISL_18428623, Singapore) Sampled Countries: Singapore (11)

Genomes: `EPI_ISL_18355035, EPI_ISL_18361806, EPI_ISL_18361914, EPI_ISL_18361935, EPI_ISL_18362103, EPI_ISL_18397258, EPI_ISL_18397453, EPI_ISL_18428554, EPI_ISL_18428623, EPI_ISL_18428638, EPI_ISL_18428659`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_c933_9f2590.json?label=id:node_3474466 image

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Branch 69

Defining Mutations: EG.5.1.1 > G1085T(ORF1a:V274L) > G1535A(ORF1a:V424I), G22927T(S:L455F) Query: G1085T, G13627A, G22927T, -A11000G, -C29375T, Earliest seq: 2023-10-02 (EPI_ISL_18361916, Singapore) Latest seq: 2023-10-16 (EPI_ISL_18428661, Singapore) Sampled Countries: Singapore (5)

Genomes: `EPI_ISL_18361916, EPI_ISL_18397376, EPI_ISL_18397599, EPI_ISL_18428562, EPI_ISL_18428661`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_18f8d_9f5e00.json?label=id:node_3486178 image

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Branch 70

Defining Mutations: EG.5.1.1 > T21748C(S:V62) > A16225G(ORF1b:T920A), T25036C > G22927C(S:L455F) Query: A16225G, T21748C, G22927C Earliest seq: 2023-08-10 (EPI_ISL_18438105, Beijing, China) Latest seq: 2023-10-09 (EPI_ISL_18426675, Montana, USA) Sampled Countries: China (13: BJ/11; HB/1; HL/1), Netherlands (2), Singapore (1), USA (1: MT)

Genomes: `EPI_ISL_18254239, EPI_ISL_18331365, EPI_ISL_18362093, EPI_ISL_18384410, EPI_ISL_18386767, EPI_ISL_18426675, EPI_ISL_18438074-18438075, EPI_ISL_18438082, EPI_ISL_18438103, EPI_ISL_18438105, EPI_ISL_18438108, EPI_ISL_18438114, EPI_ISL_18438136, EPI_ISL_18438177-18438178, EPI_ISL_18438235`

UShER: https://nextstrain.org/fetch/genome-test.gi.ucsc.edu/trash/ct/subtreeAuspice1_genome_test_4eb1c_b77620.json?label=id:node_3464035 image

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Branch 71

Defining Mutations: EG.5.1.1 > C6638T(ORF1a:H2125Y) > G22927T(S:L455F) Query: C6638T, G13627A, G22927T, -C14889T Earliest seq: 2023-09-11 (EPI_ISL_18313157, Hubei, China) Latest seq: 2023-10-09 (EPI_ISL_18438431, Hubei, China) Sampled Countries: China (5: HB), South Korea (1)

Genomes: `EPI_ISL_18313157-18313158, EPI_ISL_18382135, EPI_ISL_18438389, EPI_ISL_18438415, EPI_ISL_18438431`

UShER: https://nextstrain.org/fetch/genome-test.gi.ucsc.edu/trash/ct/subtreeAuspice1_genome_test_b5b8_b7aee0.json?f_userOrOld=uploaded%20sample&label=id:node_3482675 image

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Branch 72

Defining Mutations: EG.5.1.1 > C11779T > G10261A > C9611T(ORF1a:L3116F), C18348T > G22927T(S:L455F) Query: C9611T, C11779T, G22927T Earliest seq: 2023-09-06 (EPI_ISL_18313115, Hubei, China) Latest seq: 2023-10-09 (EPI_ISL_18406324, Vienna, Austria) Sampled Countries: South Korea (6), Singapore (2), Austria (1), China (1: HB)

Genomes: `EPI_ISL_18313115, EPI_ISL_18355007, EPI_ISL_18355538, EPI_ISL_18376812, EPI_ISL_18381927, EPI_ISL_18382266, EPI_ISL_18406324, EPI_ISL_18408737, EPI_ISL_18435658, EPI_ISL_18439298`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_3b3db_cbcfb0.json?label=id:node_3497922 image

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Branch 73

Defining Mutations: EG.5.1.1 > C19170T > G22927T(S:L455F) Query: G13627A, C19170T, G22927T, -C1909T, -C3736T, -C5031T, -C7504T, -A18153G Earliest seq: 2023-08-31 (EPI_ISL_18438072, Beijing) Latest seq: 2023-10-14 (EPI_ISL_18442753, Sichuan) Sampled Countries: China (6: SC/3; BJ/1; HB/1; SC/1), South Korea (1)

Genomes: `EPI_ISL_18320875, EPI_ISL_18360251, EPI_ISL_18360272, EPI_ISL_18438072, EPI_ISL_18438352, EPI_ISL_18439041, EPI_ISL_18442753`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_26c5d_cced10.json?label=id:node_3466266 image

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Branch 74

Defining Mutations: EG.5.1.3 > A28032G(ORF8:I47V) > A1047C(ORF1a:K261T) > C3608A(ORF1a:L1115I), C18261T, G22927T(S:L455F) Query: A1047C, C1889T, G22927T Earliest seq: 2023-09-11 (EPI_ISL_18314192, France) Latest seq: 2023-10-12 (EPI_ISL_18441710, Netherlands) Sampled Countries: Belgium (2), France (2), Netherlands (1)

Genomes: `EPI_ISL_18314192, EPI_ISL_18337916, EPI_ISL_18338399, EPI_ISL_18399343, EPI_ISL_18441710`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_38cb6_cd6010.json?f_userOrOld=uploaded%20sample&label=id:node_3458506 image

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Branch 75

Defining Mutations: EG.5.1.1 > C29253T(N:S327L) > G22927T(S:L455F) Query: G13627A, G22927T, C29253T, -G1067T, -C2110T, -A2730C Earliest seq: 2023-08-17 (EPI_ISL_18389043, Xinjiang, China) Latest seq: 2023-10-24 (EPI_ISL_18445150, Singapore, travel history: Malaysia) Sampled Countries: Denmark (3), China (2: XJ), Singapore (2)

Genomes: `EPI_ISL_18320957, EPI_ISL_18377581, EPI_ISL_18377610, EPI_ISL_18389043, EPI_ISL_18397195, EPI_ISL_18442952, EPI_ISL_18445150`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_1c2e8_a8650.json?label=id:node_3492354 image

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Branch 76

Defining Mutations: EG.5.1.1 > T26609C > C5907T(ORF1a:T1881I), T17454A > G22927T(S:L455F) Query: G13627A, T17454A, G22927T Earliest seq: 2023-09-20 (EPI_ISL_18445852, Castilla y León, Spain) Latest seq: 2023-10-13 (EPI_ISL_18402965, Donostia-San Sebastián, Spain) Sampled Countries: Spain (9)

Genomes: `EPI_ISL_18402961, EPI_ISL_18402965, EPI_ISL_18419672, EPI_ISL_18421286, EPI_ISL_18445852, EPI_ISL_18445906, EPI_ISL_18445934, EPI_ISL_18446071-18446072`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_3b023_af570.json?label=id:node_3474073 image

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Branch 77

Transferred to main repo _Transferred to cov-lineages/pango-designation#2401_ **Defining Mutations:** `EG.5.1.6` > `C925T, C27161T` > `C14919A` > `G14559A` > `G22927T(S:L455F)` **Query:** `C5835T, G14559A, G22927T` **Earliest seq:** 2023-09-11 (`EPI_ISL_18381025`, British Columbia, Canada) **Latest seq:** 2023-11-21 (`EPI_ISL_18572342`, Israel) **Sampled Countries:** Canada (59: AB/25; BC/25; SK/7; ON/1; QC/1), Israel (5), USA (3: NY/2; CA), Finland (2), Sweden (2), Austria (1), Ireland (1), UK (1: EN)
Genomes: `EPI_ISL_18313726, EPI_ISL_18374552, EPI_ISL_18381025, EPI_ISL_18381642, EPI_ISL_18399548, EPI_ISL_18405416, EPI_ISL_18429724, EPI_ISL_18431316, EPI_ISL_18431484, EPI_ISL_18431577, EPI_ISL_18448820, EPI_ISL_18449315, EPI_ISL_18451058, EPI_ISL_18452675, EPI_ISL_18453011, EPI_ISL_18453157, EPI_ISL_18456002, EPI_ISL_18456201, EPI_ISL_18493062, EPI_ISL_18499142, EPI_ISL_18501414, EPI_ISL_18501469, EPI_ISL_18501539, EPI_ISL_18501675, EPI_ISL_18512520, EPI_ISL_18512575, EPI_ISL_18512726, EPI_ISL_18512904, EPI_ISL_18512975, EPI_ISL_18513012, EPI_ISL_18516832, EPI_ISL_18516837, EPI_ISL_18517029, EPI_ISL_18517372, EPI_ISL_18517374-18517375, EPI_ISL_18517477, EPI_ISL_18517505, EPI_ISL_18526773, EPI_ISL_18526937-18526938, EPI_ISL_18526942, EPI_ISL_18526999, EPI_ISL_18527195, EPI_ISL_18532450-18532451, EPI_ISL_18533407, EPI_ISL_18538515, EPI_ISL_18538528, EPI_ISL_18538535, EPI_ISL_18538740, EPI_ISL_18538774, EPI_ISL_18538986, EPI_ISL_18541507-18541508, EPI_ISL_18547665, EPI_ISL_18552699, EPI_ISL_18553923, EPI_ISL_18553948, EPI_ISL_18558921, EPI_ISL_18560813, EPI_ISL_18563933, EPI_ISL_18563975, EPI_ISL_18563992, EPI_ISL_18564064, EPI_ISL_18564086, EPI_ISL_18564088, EPI_ISL_18564119, EPI_ISL_18564154, EPI_ISL_18564182, EPI_ISL_18564194, EPI_ISL_18572342, EPI_ISL_18572380, EPI_ISL_18572384`
**UShER:** https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_2d8f9_c314c0.json?label=id:node_3469419 ![image](https://github.com/cov-lineages/pango-designation/assets/125747944/c6c9b3f6-d7ed-46ce-a522-6c7068b9e27b)

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Branch 78

Defining Mutations: EG.5.1.1 > C18395T(ORF1b:A1643V) > G22927T(S:L455F) Query: C6541T, C18395T, G22927T Earliest seq: 2023-08-16 (EPI_ISL_18146006, Hubei, China) Latest seq: 2023-10-15 (EPI_ISL_18453170, British Columbia, Canada) Sampled Countries: Canada (7: BC), China (6: FJ/3; HB/2; GS/1), Japan (2)

Genomes: `EPI_ISL_18146006, EPI_ISL_18330196, EPI_ISL_18330296, EPI_ISL_18330311, EPI_ISL_18376315, EPI_ISL_18405472, EPI_ISL_18409239, EPI_ISL_18425055, EPI_ISL_18452695, EPI_ISL_18452923, EPI_ISL_18452979, EPI_ISL_18453059, EPI_ISL_18453168, EPI_ISL_18453170, EPI_ISL_18454186`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_3e744_1f9880.json?label=id:node_3476703 image

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Branch 79

Defining Mutations: EG.5.1.1 > T6736C > T23287C(S:A575) > C22075T(S:V171) > C8389T, A16668G, C17474T(ORF1b:T1336I), G22927T(S:L455F), G26416T(E:V58F) Query: T6736C, G13627A, G22927T Earliest seq: 2023-08-17 (EPI_ISL_18241549, Yunnan) Latest seq: 2023-10-14 (EPI_ISL_18454380, Yunnan) Sampled Countries: China (12: YN)

Genomes: `EPI_ISL_18241549, EPI_ISL_18384462, EPI_ISL_18384468, EPI_ISL_18384485, EPI_ISL_18384488, EPI_ISL_18384501, EPI_ISL_18384510, EPI_ISL_18384514, EPI_ISL_18384522, EPI_ISL_18454363, EPI_ISL_18454380, EPI_ISL_18454383`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_6626_1fc330.json?label=id:node_3476751 image

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Branch 80

Defining Mutations: EG.5.1.1 > C8146T > G2035A, G6373T(ORF1a:M2036I), G22927T(S:L455F) Query: C8146T, G13627A, G22927T, -C27557T Earliest seq: 2023-08-28 (EPI_ISL_18289791, Anhui, China) Latest seq: 2023-10-07 (EPI_ISL_18454332, Yunnan, China) Sampled Countries: South Korea (6), China (3: AH, HB, YN)

Genomes: `EPI_ISL_18289791, EPI_ISL_18376503, EPI_ISL_18382123, EPI_ISL_18454332, EPI_ISL_18460781, EPI_ISL_18460783, EPI_ISL_18460821-18460822, EPI_ISL_18460834`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_1d9d0_762270.json?label=id:node_3494057 image

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Branch 81

Defining Mutations: EG.5.1.1 > C2857T > G22927T(S:L455F) Query: C2857T, G13627A, G22927T, -C5031T, -T17475C Earliest seq: 2023-09-14 (EPI_ISL_18388886, Hubei, China) Latest seq: 2023-10-13 (EPI_ISL_18461038, South Korea) Sampled Countries: China (7: TW/5; HB; SC), Belgium (1), Japan (1), South Korea (1)

Genomes: `EPI_ISL_18388886, EPI_ISL_18399792, EPI_ISL_18427833, EPI_ISL_18432501, EPI_ISL_18438519, EPI_ISL_18440359, EPI_ISL_18440668, EPI_ISL_18461038, EPI_ISL_18461507, EPI_ISL_18461514`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_5f66_766bb0.json?label=id:node_3485431 image

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Branch 82

Defining Mutations: EG.5.1.1 > T16494C, T22307C > G22927C(S:L455F) Query: G13627A, T16494C, G22927C Earliest seq: 2023-09-12 (EPI_ISL_18376962, Singapore, Travel History: Malaysia) Latest seq: 2023-10-09 (EPI_ISL_18461687, New South Wales, Australia) Sampled Countries: Singapore (7), China (2: HL), Australia (1: NSW)

Genomes: `EPI_ISL_18355070, EPI_ISL_18355078, EPI_ISL_18355084, EPI_ISL_18355119, EPI_ISL_18376962, EPI_ISL_18397262, EPI_ISL_18397335, EPI_ISL_18461687, EPI_ISL_18465032, EPI_ISL_18465036`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice14_genome_1351a_73edc0.json?label=id:node_3508527 image

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Branch 83

Defining Mutations: EG.5.1.1 > C29358T(N:T362I) > T23803C(S:T747T) > C842T(ORF1a:P193S), C15141T, T16743C, C22450T(S:L296L), G22927T(S:L455F), C24442T(S:N960N), C25916T(ORF3a:T175I), C28232T Query: G22927T, T23803C, C29358T Earliest seq: 2023-09-11 (EPI_ISL_18384535, Yunnan, China) Latest seq: 2023-10-31 (EPI_ISL_18473684, Singapore) Sampled Countries: Singapore (11), France (2), Australia (1: NSW), Canada (1: ON), China (1: YN), Germany (1)

Genomes: `EPI_ISL_18327437, EPI_ISL_18384535, EPI_ISL_18423274, EPI_ISL_18428663, EPI_ISL_18455125, EPI_ISL_18461961, EPI_ISL_18472175, EPI_ISL_18473491, EPI_ISL_18473552, EPI_ISL_18473557, EPI_ISL_18473565, EPI_ISL_18473584, EPI_ISL_18473600, EPI_ISL_18473608, EPI_ISL_18473617, EPI_ISL_18473635, EPI_ISL_18473684`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_33d1_b55240.json?label=id:node_3483621 image

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Branch 84

Defining Mutations: HK.19 > C2455T > G22927T(S:L455F), C23533T(S:N657) Query: C2455T, C22033A, G22927T, -G25606A Earliest seq: 2023-09-20 (EPI_ISL_18327665, SEA airport, travel history: China) Latest seq: 2023-10-06 (EPI_ISL_18454355, Yunnan China) Sampled Countries: China (7)

Genomes: `EPI_ISL_18327626, EPI_ISL_18327665, EPI_ISL_18454355, EPI_ISL_18475173-18475174, EPI_ISL_18481030, EPI_ISL_18481032`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice2_genome_14b93_98620.json?label=id:node_3515333 image

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Branch 85: Mostly with ORF6:N34K/C27303A

Defining Mutations: EG.5.1.1 > C7390T > G22927T(S:L455F) Query: C7390T, G13627A, G22927T Earliest seq: 2023-08-31 (EPI_ISL_18312335, South Korea) Latest seq: 2023-10-30 (EPI_ISL_18494555, Denmark) Sampled Countries: China (7: SC/3; HB/2; XJ/2), South Korea (4), Australia (2), Denmark (1), Spain (1)

Genomes: `EPI_ISL_18284798, EPI_ISL_18312335, EPI_ISL_18312767, EPI_ISL_18313150, EPI_ISL_18330217, EPI_ISL_18355417, EPI_ISL_18361365, EPI_ISL_18376681, EPI_ISL_18402975, EPI_ISL_18447796, EPI_ISL_18461975, EPI_ISL_18475119, EPI_ISL_18481228, EPI_ISL_18494555, EPI_ISL_18495106`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_25e0e_b6750.json?label=id:node_3492892 image

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Branch 86: many with S:N17T/A21612C

Defining Mutations: EG.5.1.1 > G25855T(ORF3a:D155Y) > G22927T(S:L455F) Query: A21612C, G22927T, G25855T Earliest seq: 2023-08-31 (EPI_ISL_18284769, Sichuan, China) Latest seq: 2023-10-24 (EPI_ISL_18458002, Kuala Lumpur, Malaysia) Sampled Countries: China (9: YN/6; SC/3), Malaysia (3), Japan (1)

Genomes: `EPI_ISL_18284769, EPI_ISL_18324153, EPI_ISL_18324208, EPI_ISL_18324235, EPI_ISL_18384546, EPI_ISL_18419659, EPI_ISL_18419662, EPI_ISL_18454181, EPI_ISL_18458002, EPI_ISL_18475350, EPI_ISL_18481259, EPI_ISL_18488097, EPI_ISL_18488110`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_372c6_bbb70.json?label=id:node_3488769 image

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Branch 87

Defining Mutations: EG.5.1 > C15960T > C16260T > G22927T(S:L455F), C24771T(S:A1070V), C25658T(ORF3a:T89I) Query: C15960T, C16260T, A18492G, G22927T Earliest seq: 2023-09-01 (EPI_ISL_18384575, Yunnan, China) Latest seq: 2023-10-27 (EPI_ISL_18493260, Alberta, Canada) Sampled Countries: China (5: SH/3; SX; YN), Canada (3: AB), South Korea (2)

Genomes: `EPI_ISL_18384575, EPI_ISL_18426071, EPI_ISL_18436785, EPI_ISL_18460945-18460946, EPI_ISL_18475132, EPI_ISL_18481223, EPI_ISL_18493260, EPI_ISL_18495087, EPI_ISL_18495466`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice95_genome_43bfd_a83e0.json?label=id:node_3517572 image

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Branch 88

⚠️ sibling to Branch 32 please check potential misplacement

Defining Mutations: EG.5.1.1 > T4579A > A4576T > G22927T(S:L455F) Query: A4576T, G13627A, G22927T Earliest seq: 2023-08-18 (EPI_ISL_18239385, South Korea) Latest seq: 2023-10-30 (EPI_ISL_18520721, Saitama, Japan) Sampled Countries: South Korea (39), Japan (2), USA (2, ex. Japan), China (1, ex. S. Korea)

Genomes: `EPI_ISL_18239385, EPI_ISL_18313064, EPI_ISL_18320761, EPI_ISL_18354110, EPI_ISL_18355244, EPI_ISL_18355256, EPI_ISL_18355270, EPI_ISL_18355539, EPI_ISL_18368435, EPI_ISL_18368571, EPI_ISL_18382929, EPI_ISL_18408987, EPI_ISL_18411143, EPI_ISL_18432570, EPI_ISL_18439431, EPI_ISL_18460891, EPI_ISL_18460981, EPI_ISL_18461196, EPI_ISL_18503870, EPI_ISL_18510989, EPI_ISL_18511173, EPI_ISL_18511218, EPI_ISL_18511236, EPI_ISL_18511259, EPI_ISL_18511379, EPI_ISL_18511388, EPI_ISL_18511531, EPI_ISL_18511534, EPI_ISL_18511636, EPI_ISL_18511644, EPI_ISL_18511659, EPI_ISL_18511666, EPI_ISL_18511675, EPI_ISL_18511683, EPI_ISL_18520721, EPI_ISL_18535535, EPI_ISL_18539100, EPI_ISL_18539189, EPI_ISL_18539430, EPI_ISL_18539531, EPI_ISL_18539554, EPI_ISL_18539681, EPI_ISL_18539751, EPI_ISL_18539824`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_2459b_3b16b0.json?f_userOrOld=uploaded%20sample&label=id:node_3488766

view from EG.5.1.1 > T4579A branch, sibling to Branch 32? 3288

details image

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Branch 89

Defining Mutations: EG.5.1.1 > C27406T(ORF7a:L5F) > G22927T(S:L455F) Query: C6541T, G13627A , G22927T, C27406T Earliest seq: 2023-08-11 (EPI_ISL_18145981, Hubei, China) Latest seq: 2023-10-18 (EPI_ISL_18487967, Henan, China) Sampled Countries: China (6: HA/2; HB/2; SC/2; BJ), South Korea (1)

Genomes: `EPI_ISL_18145981, EPI_ISL_18320871, EPI_ISL_18360327, EPI_ISL_18382216, EPI_ISL_18438321, EPI_ISL_18485262, EPI_ISL_18487967`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice16_genome_15d09_3ad150.json?label=id:node_3506957 image

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Branch 90

Defining Mutations: EG.5.1.1 > C13724T(ORF1a:A4487V), A25636T(ORF3a:N82Y) > T10750C, G22927T(S:L455F) Query: G13627A, C13724T, G22927T, -C5031T Earliest seq: 2023-08-22 (EPI_ISL_18272130, South Korea) Latest seq: 2023-10-19 (EPI_ISL_18451971, SEA airport, travel history: South Korea) Sampled Countries: South Korea (6), USA (2: ex. South Korea)

Genomes: `EPI_ISL_18272130, EPI_ISL_18376686, EPI_ISL_18376765, EPI_ISL_18451971, EPI_ISL_18460748, EPI_ISL_18488908-18488909, EPI_ISL_18492194`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_42b73_3bb260.json?label=id:node_3520099 image

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Branch 91

Defining Mutations: EG.5.1.1 > C22987T(S:A475A) > G22927T(S:L455F) > G19419A, G21974C(S:D138H) Query: G13627A, G19419A, G21974C Earliest seq: 2023-08-24 (EPI_ISL_18284675, Beijing, China) Latest seq: 2023-11-04 (EPI_ISL_18486471, Dublin, Ireland) Sampled Countries: China (6: SC/4; BJ; YN), Ireland (2), Greece (1)

Genomes: `EPI_ISL_18284675, EPI_ISL_18409255, EPI_ISL_18409302, EPI_ISL_18429520, EPI_ISL_18438109, EPI_ISL_18442759, EPI_ISL_18454352, EPI_ISL_18486471, EPI_ISL_18490986`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice2_genome_6555_3bdc60.json?label=id:node_3502929 image

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Branch 92

Defining Mutations: EG.5.1.1 > C629T(ORF1a:L122F) > A1168G > C22987T(S:A475A) > T595C, C20148T, G22927T(S:L455F) Query: A1168G, G22927T, C22987T Earliest seq: 2023-09-08 (EPI_ISL_18330247, Hubei, China) Latest seq: 2023-10-30 (EPI_ISL_18539359, South Korea) Sampled Countries: South Korea (5), Japan (2), China (1, HB), USA (1: CA)

Genomes: `EPI_ISL_18330247, EPI_ISL_18435755, EPI_ISL_18443954, EPI_ISL_18446528, EPI_ISL_18502023, EPI_ISL_18503555, EPI_ISL_18511230, EPI_ISL_18520709, EPI_ISL_18539359`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_6555_3bdc50.json?label=id:node_3498262 image

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Branch 93

Defining Mutations: EG.5.1 > G26051T(ORF3a:S220I) > G9269A(ORF1a:G3002S) > T1216C, A9034G, A12121T, A12519G(ORF1a:D4085G), G22927T(S:L455F) Query: G9269A, G22927T, G26051T Earliest seq: 2023-10-21 (EPI_ISL_18453659, South Australia) Latest seq: 2023-11-05 (EPI_ISL_18524643, Queensland) Sampled Countries: Australia (7: QLD/6; SA)

Genomes: `EPI_ISL_18453659, EPI_ISL_18489126, EPI_ISL_18501702, EPI_ISL_18501758, EPI_ISL_18524643, EPI_ISL_18524655, EPI_ISL_18524668`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice52_genome_199a5_39a640.json?label=id:node_3459700 image

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Branch 94

a sub-branch of #1074 proposed by @aviczhl2

Defining Mutations: EG.5.1.13 > _G670T_rev(ORF1a:R135S_rev)_ > T28861C > G8889A(ORF1a:R2875H) > C13381T > G22424T(S:A288S) > C25047T(S:P1162L) > G25855T(ORF3a:D155Y) > G22927T(S:L455F) Query: G8889A, C13381T, G22927T Earliest seq: 2023-10-10 (EPI_ISL_18486858, Temuco, Chile) Latest seq: 2023-11-26 (EPI_ISL_18582164, Ontario, Canada) Sampled Countries: Canada (14: ON/13; NS), Chile (3), USA (3: NY/2; PA)

Genomes: `EPI_ISL_18483588, EPI_ISL_18483657, EPI_ISL_18486858, EPI_ISL_18523437, EPI_ISL_18534577, EPI_ISL_18548162, EPI_ISL_18581551-18581554, EPI_ISL_18581688, EPI_ISL_18581705, EPI_ISL_18582159, EPI_ISL_18582162, EPI_ISL_18582164, EPI_ISL_18582914, EPI_ISL_18582921-18582922, EPI_ISL_18582983, EPI_ISL_18602138`

UShER: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_7d45_55cdd0.json?label=id:node_3474267 image

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Singlets

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Doublets

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Triplets

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Quadruplets

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Quintuplets

Fujian/FCDC-QZ08S024/2023|EPI_ISL_18145964|2023-08-18; England/CLIMB-CM7YJZ4C/2023|EPI_ISL_18392773|2023-10-05; Yunnan/YNCDC-3090/2023|EPI_ISL_18454344|2023-10-12; Yunnan/YNCDC-3139/2023|EPI_ISL_18488099|2023-10-12; Singapore/R78MR69/2023|EPI_ISL_18444918|2023-10-25; South_Korea/KDCA273761/2023|EPI_ISL_18535687|2023-10-31 and USA/MN-MDH-37408/2023|EPI_ISL_18510684|2023-11-01 EG.5.1.1 > C25578T > G22927T(S:L455F) [Query: C8320T, G22927T, C25578T, 5 seqs, 11/23]

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xz-keg commented 1 year ago

@AngieHinrichs May I suggest for a slight upgrade on usher, as it always have one branch fully-sampled and other branches downsampled, can it highlight the fully-sampled branch so that we can see more clearly which branch belongs to which?

Currently the biggest problem on analysis EG.5.1.1 samples is that when uploading seqs, there suddenly come up 30+ sub-branches, with each having one fully-sampled branch and other branches downsampled. However, it is really hard to distinguish which branch is fully-sampled and which is downsampled. They look all the same at the first glance.

FedeGueli commented 1 year ago

@AngieHinrichs May I suggest for a slight upgrade on usher, as it always have one branch fully-sampled and other branches downsampled, can it highlight the fully-sampled branch so that we can see more clearly which branch belongs to which?

Currently the biggest problem on analysis EG.5.1.1 samples is that when uploading seqs, there suddenly come up 30+ sub-branches, with each having one fully-sampled branch and other branches downsampled. However, it is really hard to distinguish which branch is fully-sampled and which is downsampled. They look all the same at the first glance.

i suggest to open an issue on the usher github page.

NkRMnZr commented 1 year ago

09-21 Final update, clean up singlets/doublets, adding Branch 50-51, remove Branch 29

Adding branch 50 from former doublet with 3 seqs from Anhui Adding branch 51 from former doublet with 2 seqs from Anhui and Hubei

Remove Branch 29 that has been pulled under HK.3 now

Purge multiple singlets and doublets now merged into other branches

FedeGueli commented 1 year ago

09-21 Final update, clean up singlets/doublets, adding Branch 50-51, remove Branch 29

Adding branch 50 from former doublet with 3 seqs from Anhui Adding branch 51 from former doublet with 2 seqs from Anhui and Hubei

Remove Branch 29 that has been pulled under HK.3 now

Purge multiple singlets and doublets now merged into other branches

Thank you

AngieHinrichs commented 1 year ago

@AngieHinrichs May I suggest for a slight upgrade on usher, as it always have one branch fully-sampled and other branches downsampled, can it highlight the fully-sampled branch so that we can see more clearly which branch belongs to which?

Off the top I can't think of an easy way to do this, but an approximation would be to restore the filter on uploaded sequences. If you type "up" in the Filter input, it should auto-complete to "uploaded sequences" and then you can click on that.

As I understand it, subtrees are built in two phases, each phase contributing half of the subtree size (call it "sHalf" here, so if subtree size is 5000, sHalf is 2500):

So, when you filter to just the uploaded sequences, that should show which branches have been most densely sampled. If the number of the sequences in an ancestral branch is less than sHalf then it was probably densely sampled. The leftmost node where the first phase hits the sHalf limit is less densely sampled -- much less densely sampled if it's a node with a very large number of descendants.

There is a flaw in that approach though: sometimes when we're building a tree for an uploaded sample, we randomly get other uploaded samples that were placed nearby. And I don't know how to highlight the specific sample for which the subtree was constructed. So it is possible in some cases that those other uploaded samples are on branches that are not fully sampled. Sorry there is not really a way to distinguish those aside from just trying a single sequence when in doubt.

xz-keg commented 1 year ago

So, when you filter to just the uploaded sequences, that should show which branches have been most densely sampled. If the number of the sequences in an ancestral branch is less than sHalf then it was probably densely sampled. The leftmost node where the first phase hits the sHalf limit is less densely sampled -- much less densely sampled if it's a node with a very large number of descendants.

There is a flaw in that approach though: sometimes when we're building a tree for an uploaded sample, we randomly get other uploaded samples that were placed nearby. And I don't know how to highlight the specific sample for which the subtree was constructed. So it is possible in some cases that those other uploaded samples are on branches that are not fully sampled. Sorry there is not really a way to distinguish those aside from just trying a single sequence when in doubt.

Thanks for the explanation! Is it possible to highlight the first sHalf seqs? (e.g adding a filter)

In practice the first sHalf is not always the most dense branch, when it is the 4th~30th largest branch on a batch of seqs, the most dense branch is usually the 1st-3rd even if they're randomly selected from the other sHalf.

FedeGueli commented 1 year ago

@NkRMnZr there are a few branches to put under scrutiny for a potential proposal: Branch 31, Branch 17 , Branch 15, Branch 23 , Branch 35.

FedeGueli commented 1 year ago

England/CLIMB-CM7YMX5B/2023|EPI_ISL_18227053|2023-08-27 EG.5.1.1 > C11779T > C12525T(ORF1a:T4087I) > C4021T, G22927T(S:L455F), A24340G [Query: C11779T, C12525T, G22927T, 09/06] this one now 4 also from NZL tracking it privately

Edited 2 not 4.

FedeGueli commented 1 year ago

@NkRMnZr branch 31 has 22 sequences on Usher : https://nextstrain.org/fetch/genome-test.gi.ucsc.edu/trash/ct/subtreeAuspice6_genome_test_21e4b_148150.json?label=id:node_6975795 Schermata 2023-09-25 alle 12 02 53 I think you could propose it @NkRMnZr

FedeGueli commented 1 year ago

Please propose branch 44 low numbers but a lot of spike diversity. it has to be tracked publicly

https://nextstrain.org/fetch/genome-test.gi.ucsc.edu/trash/ct/subtreeAuspice1_genome_test_3c7b_184810.json?label=id:node_6950604 Schermata 2023-09-25 alle 15 07 30

xz-keg commented 1 year ago

Branch 33 is at 22 now

NkRMnZr commented 1 year ago

I'll look into those when GISAID fix that millibogo thing

olias120676 commented 1 year ago

I don't think this one is in here yet.

GISAID query: C6016T, T23953A, G25019C

EG.5.1.1 > G22927T(S:L455F) > C6016T, T23953A (S:F797L), G25019C (S:D1153H) First: 2023-08-28 EPI_ISL_18268474 hCoV-19/Shanghai/SCDC-XG09-13182/2023

Three more from China since then (1 on 2023-09-04 & 2 on 2023-09-11) EPI_ISL_18313145 EPI_ISL_18313147 EPI_ISL_18313152

Plus not on GISAID there are 3 from England, with an additional S:Q474K Fastas for those available here

https://nextstrain.org/fetch/raw.githubusercontent.com/olias120676/ukcovid/main/json/subtreeAuspice1_genome_test_da18_422c80.json?c=userOrOld&label=id:node_6978097

Screenshot 2023-09-27 141921

NkRMnZr commented 1 year ago

I don't think this one is in here yet.

GISAID query: C6016T, T23953A, G25019C

EG.5.1.1 > G22927T(S:L455F) > C6016T, T23953A (S:F797L), G25019C (S:D1153H) First: 2023-08-28 EPI_ISL_18268474 hCoV-19/Shanghai/SCDC-XG09-13182/2023

Three more from China since then (1 on 2023-09-04 & 2 on 2023-09-11) EPI_ISL_18313145 EPI_ISL_18313147 EPI_ISL_18313152

You mean G22927C, it's branch 5 retracted from cov-lineages/pango-designation#2250 , which is less likely an artifact since more and more samples now are from labs of different countries.

NkRMnZr commented 1 year ago

Branch 44 (FLippeR) proposed cov-lineages/pango-designation#2296

After reviewing Branch 31, there are 2 outlier seqs that may be the cause of the split from HK.3

Ireland/CO-CUH-SC0333/2023|EPI_ISL_18161904|2023-08-18
Israel/ICH-741199056/2023|EPI_ISL_18300205|2023-09-14

The Irish sequence does have good coverage and it doens't seem to have S:L455F; however the Israeli one doens't cover that area, so maybe artifact/dropout. Now that the following 24 seqs with S:L455F (Branch 31) are placed under the EG.5.1.1 > C12020T, T24496C branch caused by single Irish sequence, do you think it is simply HK.3 sublineage misplaced, or an actual different one? The follow-up seqs are quite Irish/English based though. @FedeGueli @AngieHinrichs

FedeGueli commented 1 year ago

About branch 31 question: that two only sequences are a bit suspicious and this could be a well defined HK.3 sublineages. i spotted some seuqences in the tree with same nuc mutations (not all togerhter) sometimes with Orf8:A65V that is really on the top mess when popping uop in the trees.

@AngieHinrichs could you take a look please? likely excluding the two sequences listed above this branch will merge with Hk.3 resolving it.

here the tree: https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/subtreeAuspice1_genome_388dc_4b1290.json?f_userOrOld=uploaded%20sample

NkRMnZr commented 1 year ago

Branch 51 now has a FLippeR singlet Xinjiang/XJCDCBAZ-294/2023|EPI_ISL_18320892|2023-08-26

AngieHinrichs commented 1 year ago

About branch 31 question: that two only sequences are a bit suspicious and this could be a well defined HK.3 sublineages. i spotted some seuqences in the tree with same nuc mutations (not all togerhter) sometimes with Orf8:A65V that is really on the top mess when popping uop in the trees.

As usual, there's not enough info to be really sure. I expect if I prune the two sequences, then temporarily the branch would be merged into HK.3 -- but then if they are not permanently excluded, when they are added back, I expect they would pull the branch back in because sharing 3 mutations (C12020T, T24496C, C21516T) is a parsimony win over sharing just G22927T (S:L455F).

FedeGueli commented 1 year ago

Branch 23 reached 24 and it is spreading in Singapore while it has a branch with S:V171I spotted and proposed by @aviczhl2 I suggest to propose it.

FedeGueli commented 1 year ago

Branch 23 reached 24 and it is spreading in Singapore while it has a branch with S:V171I spotted and proposed by @aviczhl2 I suggest to propose it.

i ve re-edited the B23 query to exclude more convergent lineages: C6541T, G21604T, G22927T, -A518G, -C1426T, -G3663A, -C5031T, -A7849G,-T16545G,-C8664T,-C3634T and now it give 20 seqs. still worth proposing it (14H-455F )

https://nextstrain.org/fetch/genome-test.gi.ucsc.edu/trash/ct/subtreeAuspice1_genome_test_53d56_686310.json

FedeGueli commented 1 year ago

there is this EG.5.1.1 Flip tha is likely misplaced by the S:Y144F artifact: https://nextstrain.org/fetch/genome-test.gi.ucsc.edu/trash/ct/subtreeAuspice3_genome_test_53d56_686310.json?label=id:node_6904850

i put here a query that finds a part of it: C1471T, G22927T,T16545G and another larger that could be used to keep it checked once the tree will be fixed:C1471T, G22927T

xz-keg commented 1 year ago

Branch 45 seems flying image

FedeGueli commented 1 year ago

Branch 45 seems flying image

Likely an italian breed. yes agree. @NkRMnZr later or tomorrow i will check every single branch i think we can add some proposal

FedeGueli commented 1 year ago

@NkRMnZr put together please a proposal for Brnach 45 last doubling 4days very fast and going. thx @aviczhl2 for spotting.

FedeGueli commented 1 year ago

Branch 45 now 16 intercepted also in a traveller from Germany to NY

NkRMnZr commented 1 year ago

Branch 45 proposed cov-lineages/pango-designation#2308

Branch 26 seems fast too

FedeGueli commented 1 year ago

Branch 26 seems fast too

Now it is 37: Schermata 2023-10-04 alle 17 35 35 https://nextstrain.org/fetch/genome-test.gi.ucsc.edu/trash/ct/subtreeAuspice1_genome_test_260e7_d84d30.json?label=id:node_3639269

Please Propose it!

FedeGueli commented 1 year ago

@NkRMnZr please propose branch 31 it is going well in Uk with a lot of samples not yet on Gisaid (27) vs 46 on Usher Schermata 2023-10-06 alle 12 10 26 https://nextstrain.org/fetch/genome-test.gi.ucsc.edu/trash/ct/subtreeAuspice5_genome_test_309de_f8fe00.json?label=id:node_3999349

Think that both Orf1a:L3919F and Orf1a:A656V are beneficial according to Bloom/Neher calculator.

FedeGueli commented 1 year ago

@NkRMnZr spotted Branch 53 independently i was proposing but found here! thank you! i think T7111C, A20010T is a better query it catches 12 ( any query with defining 27571 finds 10 due to droputs) I think you can propose it it seems really fast: https://cov-spectrum.org/explore/World/AllSamples/Past3M/variants?nextcladePangoLineage=HK.3*&nucMutations1=T7111C%2CA20010T&analysisMode=CompareToBaseline& ( look at CIs distribution)

krosa1910 commented 1 year ago

? Is the branch with S:G184S=G22112A designated elsewhere? Does not find it here yet it is 40 sequences @NkRMnZr @FedeGueli

Inquiry: C22480T,G22112A,G22927T,T4789A https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/singleSubtreeAuspice_genome_27385_1ddad0.json?f_userOrOld=uploaded%20sample&label=id:node_1087467

Screenshot 2023-10-07 at 6 40 03 PM
FedeGueli commented 1 year ago

? Is the branch with S:G184S=G22112A designated elsewhere? Does not find it here yet it is 40 sequences @NkRMnZr @FedeGueli

Inquiry: C22480T,G22112A,G22927T,T4789A https://nextstrain.org/fetch/genome.ucsc.edu/trash/ct/singleSubtreeAuspice_genome_27385_1ddad0.json?f_userOrOld=uploaded%20sample&label=id:node_1087467 Screenshot 2023-10-07 at 6 40 03 PM

Yes it is branch 26 HK 11(184S)+ 455F

@nkrmnzr propose it please

xz-keg commented 1 year ago

South_Korea/KDCA262129/2023|EPI_ISL_18271704|2023-08-21 EG.5.1 > T26972C > C28909T > C2106A(ORF1a:T614N), A21759G(S:H66R), G22927T(S:L455F), G24004A, C25572T, G28423T(ORF9b:R47L) [Query: A18492G, G22927T, C28909T, 09/18]

This singlet grows to 6.

FedeGueli commented 1 year ago

Please propose Branch 48 : now 24 seqs widespread inmmutliple countries with further N and S mutations Schermata 2023-10-09 alle 12 09 28 https://nextstrain.org/fetch/genome-test.gi.ucsc.edu/trash/ct/subtreeAuspice1_genome_test_3b0f0_3cf480.json?c=userOrOld&label=id:node_3442179

Thank you to @krosa1910 for driving my attention on it: https://github.com/sars-cov-2-variants/lineage-proposals/issues/931

FedeGueli commented 1 year ago

branch 7 died off, Branch 15 i would include the step deeper in the tree with

ORF1a:T2906N ( C8982A), T19710C, C27213T

cause orf8:A65V is highly unreliable.

xz-keg commented 1 year ago

Branch 23 is 52 now.

NkRMnZr commented 1 year ago

Transferred Branch 23 as cov-lineages/pango-designation#2319 Transferred Branch 26 as cov-lineages/pango-designation#2320 Transferred Branch 31 as cov-lineages/pango-designation#2321 Transferred Branch 33 as cov-lineages/pango-designation#2322

FedeGueli commented 1 year ago

Transferred Branch 23 as cov-lineages/pango-designation#2319 Transferred Branch 26 as cov-lineages/pango-designation#2320 Transferred Branch 31 as cov-lineages/pango-designation#2321 Transferred Branch 33 as cov-lineages/pango-designation#2322

Thank you! great work!

FedeGueli commented 1 year ago

this now is 4 :England/CLIMB-CM7YMX5B/2023|EPI_ISL_18227053|2023-08-27; NewZealand/23YA4860/2023|EPI_ISL_18292157|2023-09-04 and Shanghai/SCDC-FX0920-021/2023|EPI_ISL_18360191|2023-09-19 EG.5.1.1 > C11779T > C12525T(ORF1a:T4087I) > G22927T(S:L455F) [Query: C11779T, C12525T, G22927T, 3 seqs, 10/06]

FedeGueli commented 1 year ago

Branch 48 reached 28 it seems going well still unsure if faster than HK.3: https://cov-spectrum.org/explore/World/AllSamples/Past3M/variants?nextcladePangoLineage=HK.3*&nucMutations1=G13627A%2CG22927T%2CA23539T%2CG22332G&analysisMode=CompareToBaseline&

FedeGueli commented 1 year ago

Branch 59 is likely just misplacement i asked Angie to look at it.

FedeGueli commented 1 year ago

Branch 46 went to 27 too. going closer to proposal threshold.

FedeGueli commented 1 year ago

Alos branch 27 went to 31

FedeGueli commented 1 year ago

branch 17 went to 35

FedeGueli commented 1 year ago

branch 15 now 42 with signs of advantage in Singapore : https://cov-spectrum.org/explore/Singapore/AllSamples/Past3M/variants?nextcladePangoLineage=HK.3*&nucMutations1=C6541T%2CG22927T%2CC28087T&analysisMode=CompareToBaseline&

FedeGueli commented 1 year ago

Branch 5 is now HK.13

FedeGueli commented 1 year ago

Branch 39 is now HK.1.1 via https://github.com/cov-lineages/pango-designation/commit/af7949950379b6e47927b8a10d59fca5aef768c7

FedeGueli commented 1 year ago

Branch 3 has been designated HK.14 via https://github.com/cov-lineages/pango-designation/commit/c7550b14adc5cff2e855903e3841458886c3073c

FedeGueli commented 1 year ago

Branch 44 is now Designated JR.1.1 (EG.5.1.11.1.1) via https://github.com/cov-lineages/pango-designation/commit/bd9a2f7e2cc8baf43ae8317b9105c62673cacd63

Over-There-Is commented 1 year ago

HK.10 reaches 35 now. Should it be redesignated?

Over-There-Is commented 1 year ago

but according to Usher, only 23 are on the C19983T> G22927T branch,