microbiomeSeq

An R package for microbial community analysis in an environmental context

This package is developed to enhance the available statistical analysis procedures in R by providing more analysis produre and visualisation of results for microbial communities data obtained from 16S rRNA. See full details of the usage and dependencies at microbiomeSeq tutorial.

Main Features

• Alpha and Beta diversity
• Differential abundance analysis.
• Effects of environmental variables on community structure.
• Co-occurence pattern analysis in community data.

Installation

Install the package with its associated dependencies and load it for usage in R.

library(devtools)
install_github("umerijaz/microbiomeSeq") 
library(microbiomeSeq)

Data format/requirement

The data is required to be a phyloseq object (of phyloseq-class) comprising taxa abundance information, taxonomy assignment, sample data which is a combination of measured environmental variables together with any categorical variables present in the samples. If the phylogenetic tree is available, it can also be part but not so relevant for most of the functionality implemented here so far. We choose to use this format since we can have enormous options for manipulating the data as we progress with the analysis and visualisations. Details of format and comprehensive manipulations of phyloseq objects are available at phyloseq page.