poly-T filtering

[ov26]

Hi! How does your model deal with poly-T stretches, does it filter them out or are the guides merely scored poorly?

[mathinic]

Hi! Poly-T stretches are not filtered out but have a negative effect on the final score. (as you suggested) If the polyT are next to the edit on both sides (polyA on the reverse respectively), filtering them out would sometimes remove all pegRNAs. Best, Nicolas