pituitary_transcriptome_analyses

• The repo contains essential scripts and datasets to reproduce bioinformatics analyses in Hou & Chan et al. 2022

1. Scripts used to process and analyze mouse pituitary UTR-seq data can be found in UTRseq_analysis/ folder.
   • Figures 1C, 1D, 2A, 2C, 2D, 2F, 2G, 3B, 4
   • Supplementary figures S1C, S2A, S3A, S4, S5
   • Table 1, 3
   • Supplementary tables S3, S5, S7
2. Scripts used to run miRNA DE analysis can be found in miRNA_DE_analysis/ folder.
   • Figures 1E, 2B, 2E
   • Supplementary figures S2B, S3C
   • Table 2
   • Supplementary tables S2, S4
3. Scripts used to run miRNA-gene correlation analysis can be found in miRNA_correlation_analysis/ folder.
   • Figure 3A
   • Supplementary table S6
4. Scripts used to process LISA results can be found in LISA_analysis/ folder.
   • Figure 3C
5. Scripts used to process and integrate single-nuclei data from Ruf-Zamojski et al. 2021 can be found in scRNAseq_analysis/ folder.
   • Supplementary figures S6A, S6B
6. Scripts used to test for enrichment of co-expression module genes within snRNA-seq data can be found in scRNAseq_analysis/ folder.
   • Supplementary figure S6C
7. Scripts used for RNA-seq deconvolution can be found in scRNAseq_analysis/ folder.
   • Figure 5A
8. Scripts used to identify sex-biased genes in cell types using scMappR can be found in scRNAseq_analysis/ folder.
   • Figure 5B
   • Supplementary table S8