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y9c / pseudoU-BIDseq

🧪 New pipeline for detecting pseudouridine modification on RNA (BID-seq, etc)
https://bidseq.chuan.science/
GNU General Public License v3.0
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header of result #7

Closed hanguojun007 closed 10 months ago

hanguojun007 commented 10 months ago

Hi @y9c , thanks very much! i have got the final result. but i don't know what mean is the depth, gap,WT_ratio,WT_fraction. could you give me a detailed explanation ? genome.zip

y9c commented 10 months ago

Hello,

In this dataset, the "depth" column represents the count of reads that cover a specific site, while the "gap" column signifies the count of reads containing gaps within that site. The "WT_ratio" column calculates the ratio of gaps to the depth for the WT sample, providing a measure of relative gap prevalence. Finally, the "WT_fraction" column represents the fraction of modification for the WT sample, taking into account the application of calibration curves.