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Hello!
I am processing ITS data with DADA2 (primers ITS1F-ITS2). I am following recommendations from this paper (https://www.sciencedirect.com/science/article/pii/S1754504818302800?via%3Dihub) and fo…
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Hi,
I am just testing the tool on my FASTQ files and always getting an error "buffer overflow detected". What does it mean?
Do FASTQ files need any preprocessing (e.g. a header change)?
Sho…
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Hi,
I have a particular case and wanted to know If I can address this with `minimap2`. I have some Illumina reads that come from several amplicons and I also have a list of primers that generated …
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Hello! I am new to CAMISIM and am running into a bit of a roadblock. I am attempting to run metagenome_from_profile.py using the default_config.ini file using the instructions that you gave in the man…
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Dear All,
Can I use Mykrobe tool for targeted sequencing instead of whole genome sequencing?
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Hi,
I've been asked if it is possible to view the product sizes in the primer database? (I presume on both primer test results and all approved primers).
Also, I think I asked this before but n…
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Hi team
I know that trimgalore is a wrapper around cutadapt but I want to check what I ran is considered fine
I ran trimgalore to remove adaptors and primers at 5' and 3' ends but then
```
trim…
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Hey all,
I am new to phyloseq and have a question about loading taxonomy in the package. I have created an ASV table using the dada2 ITS pipeline workflow [https://benjjneb.github.io/dada2/ITS_work…
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Hi all,
I just noticed that the website states what I think is incorrect information:
Q: Why do some entries have more than one light and/or heavy chain sequence?
A; We are displaying any retu…
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