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Hi,
I am trying to install funannotate on a server running ubuntu. Our institution doesn't allow us to use docker for "security reasons" and the conda install hangs indefinitely, so I went the mamb…
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Hi,
Thanks for building this great tool!
I'm trying to map some published data and I get the following error:
localrules directive specifies rules that are not present in the Snakefile:
…
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### Description of feature
It should be possible to set the following information in the samplesheet on a per-sample basis:
* expected_cells (Used at least by cellranger and starsolo, don't know…
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# 生信爱好者周刊（第 79 期）：四千周
这里记录每周值得分享的生信相关内容，周日发布。
本杂志开源（GitHub: [openbiox/weekly](https://github.com/openbiox/weekly)），欢迎提交 issue，投稿或推荐生信相关内容。
[「生信周刊讨论区」](https://github.com/openbiox/weekly/discu…
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### Description of feature
It would be great if the `kallisto` options to build an appropriate index for snRNA-seq (i.e., a custom built DNA and intron index) and to properly generate count matrices …
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For gene-level DE, we have (e.g.):
`de_summary_.csv`
`deseq2_results_fpkm_.csv`
for the main files, and:
`_de_summary_.csv`
`_deseq2_results_fpkm_.csv`
when we're output subsets of compa…
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In your pipeline, The prepare_gene_expression.R script is missing in 5.Genetic_analysis, can you provide this code? Thanks~
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**Overall question**
I was wondering about model choice (i.e., haplotypes, transcripts, or haplotype-transcripts).
**Model choice**
I have RNA-seq data for which the diplotype of each indivi…
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Is it possible to specify the `-x` option to allow for running paired-end data with the UMI on read 2, but no cell barcode? The strandedness would be `--rf-stranded` as well.
Reads look like:
Re…
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Get the following error when trying to build from source so as to maintain h5 support.
`configure: error: cannot find required auxiliary files: config.guess config.sub`