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Available here: https://www.auger.org/index.php/science/data
See also their E&O pages: https://www.auger.org/index.php/edu-outreach
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Having read the manuscript, here's my understanding of the data you're working with. Please let me know if I've got this wrong, as the rest of my comments might change.
- You're working from IDAT fil…
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**Is your feature request related to a problem? Please describe.**
When we calculate a metric like `(Fm - F0) / Fm`, there can be some quality control issues. One that came up is that when `F0 = 0` t…
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Hello,
I'm working on a project that needs to quantify fluorescence of ROIs in images using a template ROI set that ideally would be slightly modified to each image. ROI Buddy seems to be exactly the…
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# Problem
I am trying to create a LoadData CSV with an Index.xml file (I had to rename it to get the error that it wasn't named Index.idx.xml), but I am getting a weird output where it only gives '…
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Hello folks,
I am a very new user of Gate and am learning all of the uses and capabilities of the program. I am currently using version Gate-9.2 and am running into trouble trying to run optical im…
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Layer `Fast Repetition Rate Fluorometry (FRRF) data collected on the RV L'Astrolabe`
There is no metadata header. The list of variables is a bit confusing
```
FoL,FmL,FvL,Fv_Fm_ratio,SigL,pL,TauL,G…
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## 🚀 Feature
A big pet pet peeve of mine is displaying monochromatic fluorescence images with a colormap. We don't currently offer a good way to temporarily look at a single channel from a multi-lay…
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**Is your feature request related to a problem? Please describe.**
I'm often interested in metrics like the coefficient of variation of a fluorescence signal _over space_ within a single cell, which …
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Hi Thomas, when calculating the df/f, people usually use fitted control as the baseline. Why do you use signal with exponential fit here? Is there any specific reason to do so? Thank you!