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Hi!
As far as I understand, in QC step, for a given gene-gRNA pair, sceptre removes cells (both ctrl & perturbed) where the target gene count is 0.
Our team found that for our dataset, there is a …
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In case it's helpful, I think some of the documentation on Prime editing parameters could be clarified. Do let me know if it's all confusion on my side, it may very well be!
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`--prime_editing…
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https://mp.weixin.qq.com/s/WKwRF6pWwOHXsFhZ44onPw
ixxmu updated
2 weeks ago
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A couple questions:
1. I've been playing around with the latest release and I'm curious about the Bonferroni option for the grna integration strategy. When I run this, I noticed the magnitude of my e…
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efficacy.algo = 'DeepCpf1'
Tested with multiple PAM-sites like YTTC, YTTV, TTTN.
The output in the summary file, will double the last letter of the PAM site.
As an example a gRNA like TTTNCACTGC…
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I ran CRISPRESSO2 using on a CRISPR/Cas9 edited loci, while the "Allele plots" shows several INDELs, the "Allele_frequency_table" does not reflect the same INDELs and recognize the INDELs as "TRUE" fo…
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The Validation Lab is ready to share Breast Cancer data with us for integration into PPP.
The dataset adds a number of additional data points to the colorectal one (currently in PPP)-such as three d…