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Hello:
I got an assembly from MeCAT, then removed removed heterozygous redundancy and assembled scaffolds using [redundans](https://github.com/lpryszcz/redundans). Then after filling gap with PBJel…
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Hi,
I am trying to assemble corrected PacBio reads from a human sample and get the following error.
```
$ mecat2canu -trim-assemble -p simon10X -d SIMON genomeSize=3g ErrorRate=0.02 maxMemory=1…
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Hi, thanks in advance for taking the time to answer this question. What are the constraints of using SUPPA with PacBio sequencing data if any? Thanks again
aa9gj updated
3 years ago
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Have you missed me? Trying to run PacBio data through and getting the following error with quality_profile_pairs.
```
> qpp %
dadautils::write_QPP(file.path(CFG$output_path, "qpp"))
Error:…
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Hi @chhylp123,
I have both short reads (Illumina) and long reads (Pacbio HiFi) for parents. Which data do you recommend to use for hifiasm-trio mode? or merge short reads and long reads?
For ya…
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Hi
I'm trying to replicate some splice junctions in my long-read Kinnex data. Out of a total of 115 splice junctions, I managed to replicate about 100 from quantification obtained via the isoseq/SQ…
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When calculating depth by GC content, samtools stats command ignores reads overlapping boundary between two GC bins. For short read data, it doesn't matter since, as explained in [stats.c](https://git…
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Make an assembly for the _E. coli_ MG1655 genome from illumina (short read) and PacBio (long read) data sets
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HI,
I am working on a hybrid assembly for a 3.2G allotetraploid plant genome:
1. Illumina assembly with [w2rap-contigger](https://github.com/bioinfologics/w2rap-contigger).
2. [DBG2OLC](https:/…
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Hi @jason-weirather ,
Thank you for this awesome tool. I want to try it for our PacBio, Illumina, and ONT data. However, I keep on getting the error mentioned in the subject like regardless of my a…