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Hi Tim,
I moved the thread here.
My original question:
> I wonder whether you could add the option to define control cells as those having 0 gRNAs for the target. When doing assign_grna(), …
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https://mp.weixin.qq.com/s/jAkmtUW1BS44CWXEAHLzmA
ixxmu updated
3 months ago
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https://google.com/url?q=https%3A%2F%2Fcabelas.cc&sA=d&sNtz=1&usg=afQJCNhUWBoaqwJVwNOA9Gr7L7A8sIt8Iw
https://google.ad/url?q=https%3A%2F%2Fcabelas.cc&sA=d&sNtz=1&usg=afQJCNhUWBoaqwJVwNOA9Gr7L7A8sIt8I…
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Hello!
I'm back again :)
After previous successful runs of `sceptre` both in local mac and cluster (with `parallel=FALSE`), I tried changing a parameter to improve my negative control calibration.…
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Hi all,
Thanks again for generating an all-in-one annotation pipeline with great documentation!
This is in reference to issue #232, I am getting a similar error where BAKTA cannot find an existing…
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Tom's suggestion
- https://www.nature.com/articles/s41586-020-2099-x
- https://github.com/biohank/CRISPR_screen_analysis
> Effect sizes for sgRNAs were calculated as previously described [[17](…
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**Describe the bug**
Running bakta gives to following error for some fasta files. I have about 1,500 sequences and the bakta pipeline returns this error on a couple of them. I have tried updating the…
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I am running Bakta (via conda) v. 1.8.1 and have stumbled upon two bugs. I believe one (Crispr detection) is tied to Bakta, but I am not totally sure about the other one (AMR)
I am routinely runni…
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#### What are the scientific goals of the analysis?
Assessing the impact or essentiality of CLK1 on the KNS42 cell line and across brain tumor cell lines.
#### What methods do you plan to us…
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# Background
Problem emerged with commit e476eb2a68b813ff145b387939d130c5adb9696b.
# Reproducible Code
To save time,
```
from config import DIR
file_path = f"{DIR}/replogle_2022_k562_esss.…