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Hi @wdecoster
I have 2 nanopolish output tsv files (calls and frequencies). I would like to use them as input for methplotlib. I have tried to sort as you wrote in an example but when i want to us…
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Hello
I called the methylation on your example data and I ran the calculate_methylation_frequency.py tools to get the methylation_frequency.tsv file.
Now I would like to launch compare_methylation…
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Hi @hasindu2008 and all,
For the `f5c meth-freq` step, is the reommended threshold for the log likelihood ratio still 2.5, or should be 2.0 as nanopolish has changed?
Thank you in advance!
Be…
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Installation fails. Can’t access the HDF5 site. Any plans to fix this again or is nanopolish not accessible to those that didn’t install 2 years ago?
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Hi,
I have been using Nanopolish to call methylation and wondered if reads are automatically trimmed by Nanopolish when making these methylation calls? My reason for asking is that when I compare r…
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Hello,
I am trying to install the r10 branch and I get this error:
make: *** [eigen/INSTALL] Error 8
I am trying to generate a new consensus for a canu assembly. I had tried the most recent…
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See https://github.com/brewsci/homebrew-bio/search?q=%22needs+openmp%22
| Formula | PR | Merged? |
| :--- | :---: | :---: |
| `arcs` | | |
| `arks` | | |
| `biobloomtools` | | |
| `bracken` | …
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Hello,
I was wondering if you knew of any good publicly available datasets for the V3 Artic Tiling Amplicon sequencing of hCoV-19. Ideally I would love to have a test dataset showing each of the v…
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Hello,
I successfully ran uncalled4 align using the --eventalign-out parameter to obtain signal alignments. I want to use this output file as input for m6Anet dataprep, but the output files from m6…
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Hi,
I'm running MOP2 on awsbatch with a relatively minimalistic input setup for testing purposes. I have matching wildtype and invitro DRS data (1 rep each) and i defined a reference for only one g…