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I found an inconsistency in scoring of alignments: reads of length 99 or 100 get lower scores than reads of shorter lengths which originate from the same genomic position (same start, different ends).…
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Hello,
I have been trying to genotype structural variants from a graph made with minigraph-cactus, by mapping short reads with vg giraffe, then using vg pack and vg call to get a vcf. This runs wit…
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Hi,
Thanks for developing vamb; excited to try it.
Now, it seems that the mapping should be performed carefully, more so than for other binners I guess.
It seems that you often used minimap2.…
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Hi there,
I've been using isoCirc successfully for a while now but this week after installing v1.0.6, it seems to be generating some errors towards the end of the process. I am able to get isocirc.…
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Dear Alex,
Hello, Alex. Thank you for developing such a great tool. I've just started learning about bioinformatics, and I'm really interested using your tool to analyze various RNA-seq data.
Re…
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**Deliverable this task is associated with**
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**RACI**
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I am trying to align the reads from [SRR9640925](https://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR9640925) to this [tick genome](https://www.ncbi.nlm.nih.gov/assembly/GCF_013339745.2/). I generated a…
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Hi,
I am using STAR 2.7.3a for extracting unmapped reads with the --outReadsUnmapped Fastx. I found that the number of unmapped reads in both mate1/2 is different from the Log.final.out output.
T…
ghost updated
4 years ago
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Hello,
I'm using epic 0.2.9 to compare two mapping samples against a mapping reference, and I have some questions for you.
I'm looking for enriched regions of unknown size comparing two experimental…
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Hello,
I am working on RNA-seq data, that consists of 150 bp paired-end reads and I was hoping to align them to the already published reference genome of the some species. Genome size is: ~3300000…