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作者您好,您的工作对GWAS和scRNA-seq结合研究非常有帮助
自己的配置如下:
R: R_4.2.2
Seurat: 5.0.1
ggpubr: 0.6.0
目前我在windows中的Rstudio下重复示例代码时遇到如下问题:
`
devtools::install_github("sulab-wmu/scPagwas")
library(scPagwas)
sy…
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Hi,
Is there an option in determineDropoutCandidates to handle missing value?
I made a reduced UMI count matrix for my single-cell RNA-Seq data. Then I had the "missing value" issue:
>x=read.…
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Hello,
I admire your new method "PHATE" on learning the manifold structure of high dimension data, and I have tried it on our own single-cell RNA-seq dataset. I wonder would you release the code of b…
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Hi
This is Joe from Cedars-Sinai Medical Center. I am currently using your scLVM on single cell RNA-seq data to reduce the cell cycle effect. This is a very nice pipeline on single cell data analysis!…
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> A lack of tools to precisely control gene expression has limited our ability to evaluate relationships between expression levels and phenotypes. Here, we describe an approach to titrate expression o…
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Hi,
Thank you for providing this software. I have some questions about its usage that I was hoping you might be able to answer.
1. In the tutorial, bdat_initial is the gene x sample matrix for…
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PMID: 28428369
Species: Human
Tissue: Blood
Paper title: Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes and progenitors
Paper link: https://pubmed.ncbi.nlm.nih.gov…
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Project Title: Transcriptomic classification of human retinal cell types with single-nuclei RNA-seq.Project UUID: 9c20a245-f2c0-43ae-82c9-2232ec6b594fWrangler/s: Zinaida,A,Perova; PI/s: Margaret,,DeAn…
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> cat PUMATAC_tutorial-main/PUMATAC/src/singlecelltoolkit/processes/barcode_correction.nf
nextflow.enable.dsl=2
//binDir = !params.containsKey("test") ? "${workflow.projectDir}/src/singlecelltoolk…
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Single-cell RNA-seq with spike-in cells enables accurate quantification of cell-specific drug effects in pancreatic islets
https://doi.org/10.1186/s13059-020-02006-2
Data: https://www.ncbi.nlm.nih.…