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I have been trying racon out on a plant genome assembled by miniasm (raw pacbio reads), the assembled genome size is 547 Mbp (expected size 750Mbp) and the .paf file is 300Gb. I have tried giving upto…
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Estimate ploidy wrongly ! I expected it to be 2 ...
```
➜ MASURCA_all ./assemble.sh
[Fr Okt 19 15:27:18 CEST 2018] Processing pe library reads
[Fr Okt 19 15:38:33 CEST 2018] Average PE read lengt…
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Hi,
In MaSuRCA-3.2.7 the "Gap consensus" stage fails with the below error.
Not sure exactly how to fix this problem.
Thanks.
[Fri Aug 10 10:38:00 AEST 2018] Using linking mates
Using CABO…
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HapHiC is working very well so far, and now we would like to fine-tune the scaffolding (looking into more of the options, and comparing different input versions).
**My question:** Is it possible t…
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I am using HIFIASM like this:
hifiasm -o Outfile -t 37 --h1 File_Hi-C_R1.fastq.gz --h2 File_Hi-C_R2.fastq.gz Sequel.file.001.ccs.fastq.gz Sequel.file.002.ccs.fastq.gz Sequel.file.003.ccs.fastq.gz
…
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Hi,
My species is triploid and is highly hetrozygous. I used
`hifiasm --primary --n-hap 3 -t 24 -o out.asm .*.fastq.gz`
But the assembly size of my primary contings is way higher (240Mbp) t…
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Hello,
I am running MaSuRCA on a plant genome, I have PacBio and Illumina data as input.
I just noticed this notification:
```
$ ./assemble.sh
[Mon Sep 21 16:13:10 CEST 2020] Processing pe librar…
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Hi,
I was using masurca to assemble a genome. Unfortunately, I got an error which I cannot fix, can you please help to fix it.
cat: write error: Broken pipe
mkdir: cannot create directory `CA/f…
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Hello
Sorry if I am asking silly questions. I am a beginner and a bit confused with the pipeline. Is there any tutorial showing how to run this workflow? I do not know where should we add our input…
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Dear @alekseyzimin,
I'm trying to assembly insect genome using trimmed illumina and trimmed nanopore data. I got the following errors in bold. However, the assembly in flye folder is succeeded, and t…