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My 2d-SFS all have a blip at p=0.5 (see attached), which I assume is caused by lumped paralogs problem (i.e., SNPs that always appear as heterozygotes). Use HWE filter, you say - but I cannot filter t…
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Hey,
I wanted to run PepNovo+ on my mgf file with ~600k spectra. I used the following parameter file:
[pepnovoplus.txt](https://github.com/compomics/denovogui/files/14551531/pepnovoplus.txt)
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https://mp.weixin.qq.com/s/f-NdvCMEIS35eckTjA2c9w
ixxmu updated
3 weeks ago
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I have been trying to assemble a 10Mb genome with uncorrected nanopore data (3-4 chromosomes expected). We have a lot of data, is that the reason Flye fails at the end?
[2019-06-22 11:00:05] INFO: …
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Please, describe below the problem you think we face in the miRNA/isomiR naming.
Try to summarize it in 200 words. The current discussions are here:
* isomirs : https://github.com/miRTop/incubat…
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Hi,
I have a ~130kb N50 single flow cell out put from a UL ONT chemistry 10
Post inital qc Shasta identifies the following: The run finishes without errors but there is no alignment and the fasta …
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Dear Xianjie,
May I ask a naive question?
In a given SNP region, what are the criteria being used to categorize a cell as WT(0/0), heterozygote(1/0), and homozygote(1/1)?
Are they categorized…
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Hi, I'm running Breseq to compare two closely related lactobacillus strains and I am getting +10K> mutations.
During the run, I get an error saying that the strains seem much different (above 1% dif…
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Hello,
I used the tool on a low-depth genome sequence, but it returned a VCF line that lacks read support. Could you also give me a command line options to get high quality variants from the tool. …
ghost updated
5 months ago
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### Description of feature
I'm running the mosdepth module twice inside module_order as per instructions but for one of those times I want to drop one of the three figures from the global.dist.txt fi…