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I'm trying to run some nanopore test reads through mothur but am getting stuck at the very beginning. I was going to follow your pacbio example so was trying to start with fastq.info. My data are conc…
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THE PROBLEM: Cloudflare sometimes blocks our RSS fetches because we're a bot (which is fair — we are!).
THE SOLUTION: We [register with them](https://developers.cloudflare.com/bots/troubleshooting/…
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Hi,
I have a technical question regarding the trimming step. In the case that template switching reverse transcriptase is used, is it necessary to trim additional 3nt from one end to remove the ad…
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Hey guys,
I am struggling to find some good illustrations to:
`short reads`
`long reads`
`CNV`
`STR`
`SV`
`SNPs`
`indels`
Thank you very much for your amazing work!!
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## The need
I was profiling a bam file when I noticed that a massive amount of my reads were not used:
```
WARNING
====================================================
There were 553 reads pres…
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The `Storage` trait does not expose any alignment information, and one would thus assume that implementations of it would allow for unaligned reads, but `RmwNorFlashStorage` currently does not. As an …
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@willbradshaw I didn't see an issue for this, though you noted it in the code itself:
```
# NB: Current paired version can't distinguish or annotate forward vs reverse reads in these plots.
# TOD…
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### Component(s)
pkg/stanza, receiver/windowseventlog
### Describe the issue you're reporting
The `windowseventlog` receiver has a configuration parameter `max_reads` which determines the max numbe…
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Under "while True:" does "serial_read()" really need to be called twice?
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### Ask away!
Hi,
I'm running the pipeline like this:
```
nextflow run epi2me-labs/wf-metagenomics \
--fastq $fastq_pass_path \
-profile singularity \
--database_set PlusPF-8 \
--out_dir $…