-
### Operating System
Ubuntu 22.04
### Other Linux
_No response_
### Workflow Version
v1.4.0
### Workflow Execution
Command line (Cluster)
### Other workflow execution
_No response_
### EPI2M…
-
**Describe the issue**
I am executing this command as per 'box 2' in Nat. Biot. 2024 article:
~$ kb ref -i fpa_index.idx -g fpa_t2g.txt -f1 fpa_cdna.fasta cds_from_genomic.fasta genomic.gtf
**W…
-
Hi there,
Just wondering if you have thoughts on using tools to orient bulk reads before alignment with minimap2. From what I understand in the FLAMES documentation, reads from the FASTQ are direct…
-
We are currently using canine cDNA to create a generic exome sketch. Each exome kit seems to have its own range of possible enrichment e.g. Agilent SureSelect achieves 45-75% coverage, Nimblegen Exome…
-
Dear developers,
Recently I am trying some customized methods to complete the full-length measurement of the polya tail on nanopore.
However, I don’t know whether it is because the same primer is …
-
# 运行代码
$ source ~/dnbc4tools/dnbc4tools2.1.3/sourceC4.bash
$ genome=~/dnbc4tools/GRCh38_gencode_v44_ref/
$ ~/dnbc4tools/dnbc4tools2.1.3/dnbc4tools rna run --name D2_1 \
> --cDNAfastq1 ./HRR1822706…
-
when running vanilla pepatac, I got this message.
Is there a way to streamline this? Like, maybe aggregate these and then print one list instead of duplicating the message?
```
## [1 of 16] sam…
-
Hi, we have sequenced 5 samples' scRNA-seq in your company and when I got the data, I found that there are multiple directories under each sample and for each sub-directory, there are also two pairs o…
-
Are you interested in learning more about GPU programming and developing cool optimizations? Do you want to help build next generation and state-of-the-art machine learning models and layers? Do you w…
-
Hi Brian,
I followed the steps, but failed at the final step:
`~/miniconda3/opt/transdecoder/util/cdna_alignment_orf_to_genome_orf.pl transcripts.fasta.transdecoder.gff3 transcripts.gff3 transcript…