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我可不可以自己对raw matrix进行预处理和过滤
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I read in Lior's preprint X thread that splitcode would work well with long-reads, do you have any example of how to implement it for this case?
I would like to test it to demultiplex single-cell lo…
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Filled together with @Karl-Svard
As a production user who encounters a sample sheet of a flow cell with **one sample per lane** and that has no index in its samples,
I want the sample sheet not to f…
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Hi all,
I'm using the barcoding kit SQK-NBD114.24 for sequencing on MinIon Flow cell. Before the library preparation and sequencing, I'm doing on each samples a pcr with barcoded primers:
![image]…
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Hello,
I am relatively new in variant calling using scRNA-Seq. I have 17 datasets from 17 patients. I want to call the variants for each patient. I only need the list of variants in each sample.
Can…
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Hi, Thanks for developing this helpful tool.
I am running mates on 10X data and I am on my first step which is:
`bam_processor.split_bam_files('10X', 20, 'sample_list_file.txt', 'bam_path_file.t…
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Dear Dr. Li, I have encountered some problems while studying TRUST4 script commands recently. I hope to receive your guidance:
About barcoderep-filter. py, the first two lines of the report. out in m…
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Hi, I have some unpaired scRNA-seq and scATAC-seq data, they are sequencing the same samples, but in separate scRNA-seq and scATAC-seq assays, so the cell barcodes are different in these datasets. How…
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Hello,
do the visualizations work also for the bulk_long_pipeline?
It is not so clear in the vignette.
Best,
Vasiliki
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Hello!
Thank you so much for the fascinating work here! I'm interested in looking into the WT scRNA-seq mouse gastrulation data, which I understood is deposited under GSE122187, and the correspondi…