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I didn't find any information about how did you generate these two files: cbc_gbc_dict_grna.tsv, cbc_gbc_dict.tsv, it can't be generated from 10X genomic cell_ranger. I know these two files are the in…
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In the SD2 program, we have SBOL genetic network models that have instances of guide RNAs (tokens), and what seem to be prototypical entities (types) as well, e.g., "r6 gRNA."
The types and tokens …
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Hello,
I have an issue with running the CalcPerturbSig on my own data.
seurat_objcompass_perturb
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Hi Aaron:
Been using FlashFry for quite a few years now. In my current project, I am designing millions of gRNAs and before we synthesize them, we filter the gRNAs based on GC% and homopolymeric se…
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I tried to construct an `Interaction` using the documented API, and got this error:
```
>>> inter = sbol.Interaction(types=["http://identifiers.org/biomodels.sbo/SBO:0000170"], name="BE stimulates…
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Hi Tim,
I am having some trouble with the Nextflow pipeline, I am getting diffent genes and gRNA groups failing precomputations every time (a different one) with the same error "ondisc_matrix has 0…
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Hello, is it possible to run EditR via command line via e.g., passing the gRNA sequence and the .ab1 as input?
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**What is the SO term name and accession?**
gRNA_gene (SO:0001264)
**Describe what you would like to change.**
Current def is "A noncoding RNA that guides the insertion or deletion of uridine res…
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Just to leave a comment for anyone who plan to identify gRNA in non-reference genomes.
I got same issue as this one, #2 , it seems the version of scikit-learn was the problem, can't figure out afte…
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Hi, there,
I have serveral questions. Would you please help me about them ?
I set the purpose=CRISPRa and purpose_exclusive=1, and I read the final report that some gRNA were excluded because th…