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Hi,
I wonder if bcctools also works correcting barcodes from 10x library followed by iso-seq instead of illumina seq? It would be nice if it works, since I am seeing a lot of unexpected barcodes in…
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Hi,
Is there documentation on how the CCS header file required for scallop-lr needs to be formatted?
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Dear TomSkelly:
I am sorry to disturb you.when I use the MatchAnnot, it appear IndexError.
there is the log:
[DEBUG] 2016-01-18 19:17:16,399 [Annotations 13] version 20150611.01 loaded
[DEBUG] …
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Hi,
I am trying to get STAR to work for finding fusion transcripts in PacBio (Iso-Seq) data. Previously I have been using GMAP coupled with a [downstream python script](https://github.com/PacificBios…
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Dear Longbow team,
Thank you for your great tool! It is very helpful.
Nevertheless, I am running into some problems while using it.
I am trying to annotate a hifi.bam file with longbow annota…
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Hi,
I have followed your instruction to run my data with TALC, but the running time of the program have reached 511,898 hours with 30 threads and it seems like that it don't want to stop. As mentione…
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**In general**: No. IsoCon is designed for targeted sequencing where the CCS flnc reads are cut at relatively precise positions (i.e., at the start and stop primer sites). If this is not the case it m…
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Hi,
I'm currently working with long-read RNA sequencing and encountering issues related to strand directionality. I noticed that Minimap2 appears to consider only the positive strand for transcript…
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Hi，when I run IsoCon，I found the results vary greatly with different --min_candidate_support set. So I wonder how to set this parameter is ok？
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Hi, I get this error on 180,000 sequences that are around 500-5000bp long coming from PacBio Iso-Seq data. Any ideas?
```
avg length: 2199
Recommended K: 5
Reading in sequences [> …