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Hope it finds you well.
I'm using DIA-NN 1.9 and Skyline (64 bit) 24.1.
I'm trying to use following skyline visualiation function.
_Skyline. To visualise chromatograms/spectra in Skyline, analy…
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Hi Vadim,
I've been having some trouble getting the main report.tsv file at the end of my DIA-NN run.
Maybe I'm doing something incorrectly, so I'll first just give you a step-by-step explanati…
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Hi,
I have three datasets: the first consists of 200 raw files from human blood samples, the second includes 20 raw files from bovine blood samples, and the third contains 20 raw files from mixed h…
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Hi Vadim and team,
I have done 3 plex dimethyl labeling on samples and am now trying to analyze them following the steps outlined in the plexDIA section. But as you can see below, after finished wi…
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Hi,
I have been using FragPipe to analyze some DIA data and I need decoys to appear in the 'psm.tsv' output file but I haven't been able to figure out how to get this to work. I have been able to g…
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Hi Vadim and Team,
I used a fasta file generated in silico translation, then i first used the fasta to generate a spectral library, the log of this process is shown in the first picture.
![image…
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Hello,
With the capabilities in the new version, I am interested in using DIA-NN to search for histone PTMs from extracted histones. The since histone tails are highly decorated with PTMs, the main…
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I finally have some time to take a look at v2, and I am a bit confused about how the spectral resolution is handled.... Specifically, in `sed_model.py` L131, we have
> self._library_resolution = ge…
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Hi,
I want to analyze LFQ-DIA global proteome and phospho-enriched proteome data separately on DIA-NN. Just want to make sure that we need two different libraries for these two different searches, r…