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### Overview
We are encountering some issues in obtaining appropriate estimates of error rates; I believe these problems are coming from our new(-er) sequencing facility sending us fastq files contai…
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Hi,
I have been trying to analyze my sequencing data that come from an AVITI platform instead of Illumina. The FASTQ quality score are higher than 41, which results in an error when I run the itsxp…
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For example,
Simply changing
**a**cerates viridiflora (raf.) eaton
https://api.gbif.org/v1/species/match?name=acerates%20viridiflora%20(raf.)%20eaton
to
**A**cerates viridiflora (raf.) eaton…
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Using https://docs.google.com/spreadsheets/d/1FFyRhI5TeUb-B4t50HRZYF_XngRt_mfAIz82UQ3geFk/edit#gid=0
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Dear developer:
I can't understand how to calculate the contig and bin's abundance? w…
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Hi,
I am working with a long amplicon called StrainID that includes the entire 16S rRNA gene, ITS region, and a portion of the 23S rRNA gene (~2,500 bp long), and sequenced with PacBio platforms. W…
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Hi Author(s),
I think it is super cool that you've implemented this dynamic strategy!
We where trying to normalize some amplicons on a given chromosome, so we made sequences that where unique to…
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Hi Ben,
I have a few questions regarding de-novo chimera removal.
1) What is the idea behind pooling samples for chimera removal? As chimeras are formed during PCR, when the samples are separate…
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Hi,
I ran q2-dbbact using qiime2-2021.4 and got the following error
Plugin error from dbbact: Duplicate observation IDs
The content of the log file is following
trimming primers if needed
…
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>>
>> https://github.com/rmcolq/pandora/blob/815da22867f2bdaa3e3bf40f382be830c1506b0a/src/estimate_parameters.cpp#L217-L231
>>
>> In particular https://github.com/rmcolq/pandora/blob/815da22867f2…