-
Currently, we only calculate the median and separate out high vs. low values to symbolize 1 vs. 0 for all cell health classification models. We should design a better way to binarize. Perhaps using ot…
-
This SEP proposes a ComponentReference object that allows a ComponentInstance to be referenced through children, rather than requiring it to be imported as an immediate child of the containing Compone…
-
Update the following URL to point to the GitHub repository of
the package you wish to submit to _Bioconductor_
- Repository: https://github.com/imkeller/gscreend
Confirm the following by editin…
-
I am trying to mutant a gene with two target sequences. From my preliminary data, I found there some mutations induced by CRISPR/Cas9 have indels at both target sites, but this software can only produ…
-
GEMINI: A variational Bayesian approach to identify genetic interactions from combinatorial CRISPR screens
This package is being submitted to Bioconductor upon conditional acceptance of a correspon…
-
**issue:** Need to better explain when to use deletion mutation evidence vs. knockout evidence
**deletion mutation phenotypic evidence**
**def:** A type of mutant phenotype evidence resulting from…
-
Hello
I am trying to run the CRISPRessoPooled command on the pooled deep sequencing data.
i run my script several times as per suggestion, but got an error "ERROR: Flash failed to run, please check…
-
Hi,
Using SPro to download the necessary data, I'm running to an HTTP error when attempting to grab CCLE data. I get the same error in a browser when trying to access the url which gives the error…
-
Dear Luca Pinello
I am using CRISPRessoPooled.py script to analyzed the paired ends reads data by Illumina.
the read length is 150 bp.
the amplicon size is 180bp long.
below is the command that I…
-
Environment: CentOS6.5, Python 2.7.11, CRISPResso installed from source (master.zip downloaded from github), needle, flash and trimmomatic are in the CRISPResso dependencies dir.
Data was downloaded…