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Currently, readAheadMax is a non-configurable parameter for fuse users, set by default to conf.Chunk.BlockSize * 8, typically 32MB. This isn't sufficient for low-concurrency read requests. I inten…
ddrid updated
1 month ago
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Hi all,
Thanks for developing this very interesting and useful package.
Am I correct in assuming there is no compatibility with demultiplexing cell hash data with TCR repertoire analysis (10x) …
CAC13 updated
2 years ago
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See https://forum.image.sc/t/a-couple-of-queries-about-omero-iviewer/36144
"In iviewer, is there any way of zooming in/out of the image using the microscope objective magnifications![ (10x, 40x) ra…
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Hey,
I am using cellranger version 7.0 and running the `cellranger vdj` function.
The output of `cellranger vdj` function contains a folder named `outs` (as described here [https://support.10xgenomi…
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Hi
I am starting to use Immunarch for 10x sc paired data. Thanks for implementing it!
I have loaded a dataset where my samples are defined by the Column "Cluster".
When i load the dataset, neve…
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Project: ENCODE
So - libraries for next-generation sequencing used to all be made by hand. However, we are starting to see the emergence of platforms (machines) which do the library construction i…
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Hello, I've successfully run cellranger-arc for all 10x Multiome samples, but in the .html output ([attached here](https://www.dropbox.com/s/gqrx0i9ipxe24lc/Wang2023_cellranger_html.zip?dl=0)) I get a…
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Hi,
I specified the standard output folder of the 10x SpaCET Ranger (with the "filtered_feature_bc_matrix" and "spatial" folders) in visiumPath.
As with the dataset in the tutorial,
The "filtered_…
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While trying out the file API, i noticed that file reads are incredibly slow.
I'm loading a binary file with ~60MB in size, this takes ~2.5s with `readBinaryFile`.
For comparison, loading the same …
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From the vignette and by running the SCnorm command on 10x data that is heavily zero-inflated (sequenced to 85-90% saturation), i ran into the