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Currently the rule `tagbam` adds a RG tag using `samtools addreplacerg`. The mapper `ema` apparently also adds a RG which causes an error with `buildmolecules`.
Error in `buildmolecules`
```
Trac…
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Hi,
I am trying to run the metagenomic workflow without gene calling and without external gene calls either. Basically I wanted to use only k-mer frequencies and contig coverage in multiple samples…
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I am using a Conda/GitHub installed version of DRAM; unclear which version and downloaded the databases with this command, i.e. skipping KEGG:
DRAM.py prepare_databases --output_dir DRAM_data/ --th…
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When running it on ~50 genomes I noted some of them did not get the genus and species classification that it should have gotten based on the sendsketch top hits, see attached files
[Species_prokka.tx…
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Hi,
Sorry to come up with another issue!
As a follow-up to issue #1416 I have changed the header of my BAM file(s), sorted them and then tried to run `anvi-profile`. But, while `anvi-profile` ru…
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When testing the fix for the `.Rprofile` problem, the empty file inside docker seems to have solved one issue, but now I get the following error message:
```
Error: package ‘S4Vectors’ 0.22.1 was …
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`anvi-self-test --version`:
```
Anvi'o version ...............................: esther (v6.2-master)
Profile DB version ...........................: 32
Contigs DB version .....................…
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Hi Dr. Hu,
Thank you so much for your fantastic tool! I have a question when I tried to run nextdenovo. My run.cfg is like this:
[General]
job_type = local
job_prefix = nextD_t1
task = all # …
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Hi, I'm trying to run `rgi` within a singularity container generated from the biocontainers image, but it's failing due to a missing library for `makeblastdb`.
I've tried these images so far:
```
…
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While trying to decode the attached bufr (TEMP-like vertical profile) which makes use of the [C table](https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&ved=2ahUKEwiv68ySrO_nAhXRuHEKHVzj…