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./Trinity --seqType fq --left reads2.right.fq.gz --right reads2.left.fq.gz --CPU 36 --max_memory 100G
______ ____ ____ ____ ____ ______ __ __
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Hi Nick,
I am trying to assemble mitochondrial genome for butterflies and I am getting only empty files. Also I don't get any error. Seems like it does not read my reference sequence, can you help m…
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Dear ndierckx,
I am trying running NOVOPlast, however, after hours of trying to figure out what it's going on, I gave up.
I created a Conda env called "Plant" and downloaded novoplasty from Conda …
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(ee) nbfgr@nbfgr-Precision-3650-Tower:~/Software$ NOVOPlasty4.3.1.pl -c config.txt
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NOVOPlasty: The Organelle Assembler
Version 4.3.1
Author: Ni…
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Hi,
I'm trying to run Getorg with unpaired reads. Previous runs with PE reads are not a problem. Here an overview of my commands and terminal output. Could you please point me in the right directio…
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Hi, I have the same problem as a previously mentioned issue. It presents a memory error after ROUND 3 (see below). I have increased my memory to 128gb but it is still giving me the same error. I have…
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Hello,
I am doing the plant mitochondrial genome assembly with the following commands. But I am getting a MemoryError issue. The same parameter has used for another mitochondrial genome assembly.
…
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Does the config filename have to be config.txt? I have my config files written up as config (name of sample).txt and I tried running the following command
`perl NOVOPlasty/NOVOPlasty4.1.pl -c Seque…
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Dear Nicolas Dierckxsens,
I am running NOVOPlasty 4.2 and I am trying to recover mitogenome data from adapter trimmed illumina sequence data. So far I have recovered ~1300bp, which is just slightly…