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Dear Runsheng
I am facing the following issue in running the primervcf
primerdesign_vcf.py -g cb5.fa -b QR25_del.bed --prefix QR25
mambaforge/lib/python3.10/site-packages/primer3/bindings.py:5…
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Hi,
I'm very interested in running SpeciesPrimer, however I've had limited success. I have done my best to follow the instructions, however when it comes to actually downloading the available genome…
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Dear teacher：
I am a student who is very unfamiliar with biological information. I am currently committed to cultivating dozens of different bacteria. I want to design primer of each bacteria by…
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Hi
I have a question about what should I input in bindings.designPrimers() if I want to adjust SEQUENCE_OVERLAP_JUNCTION_LIST in primer3? I have test many input types (like list[], list of list[[],…
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Hello,Runsheng
thank you for your great work.I hava ran this scripts successfully with the example data, but failed we use my own vcf as input .
I compare it to the example vcf ([QR25.vcf](https://g…
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Hi, Runsheng.
It's me again, in your latest version i could run vcf2del.py successfully. Here comes a new question.
In your primerdiffer package it describe like this :
`The C. nigoni genome is cn3…
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This sounds like a very interesting tool. I'm wondering if this tool can be used to design PCR primers targeting a (single-copy) gene sequence that is specific to my bacterial species of interest but …
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18 Oct 2021 08:44:28: > Start searching primer for Lachnoanaerobaculum_saburreum
18 Oct 2021 08:44:28: Run: collect data(Lachnoanaerobaculum_saburreum)
18 Oct 2021 08:44:28: Run gunzip /primerdesign…
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Hi there!
Thanks for this package.
I am trying to run the pipeline directly from the command line to try and understand the components. I have the `nt` database downloaded. I am currently trying t…
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Build in Windows 10 with Python 3.8:
```
ValueError: path '/Users/ben/gitrepos/primer3-py/primer3/src/primerdesign_helpers.c' cannot be absolute
```
Then I found that, in `primer3_py.egg-info/SOUR…