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Hi All,
I'm working on this project where I add light and heavy-labeled peptides together, mix them and run on an Orbitrap, using LC-DIA.
Interestingly, when I create my own peptide library usi…
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From https://sentry.galaxyproject.org/share/issue/da466f63ec5541e58db2ce827ada3918/.
I can't see any use of `$protein` in the configfile, but this is not easy to read.
These were the selection optio…
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### Description of the bug
The errors are reported when i ran PXD001819 LFQ datasets (about 10G mzML files). It looks like it's running out of memory? But the available memory is 120G. So I'm not …
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Hi Vadim,
In the protein quantification module of QuantUMS, which column in the report can be used for protein quantification?
What's difference between .parquet file andt .tsv file?
Is there…
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Dear Vadim
When I run one.mzML file on linux, I have no problem. When I run multiple.mZML files, I report an error：
ubuntu@node188:~$ ./1/diann-linux --f data2/1.mzML --f data2/2.mzML --fasta data…
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Hi. I was interested in understanding in more detail how DIA-NN implements the MaxLFQ algorithm and if it is possible to specify certain parameters in the command box.
I understand 5 fragment ions …
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Dear developers,
I am unsure why the maximum variable modification option is not working. Do you have any suggestions? I am also not getting any results when running the software. What could be the …
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[iBAQ protein quantification](https://www.nature.com/articles/nature10098) excludes missed cleavages and normalizes intensities by the number of theoretical peptides, which correlates with length.
…
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My raw files were searched with DIA-NN 1.8.1 and the output table "report.pg_matrix.tsv" was used for further analysis. I was confused with the two columns "Protein.Group" and "Protein.Ids". The colu…
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how to do label-free quantification and I cannot find the value from pBulid and the result files. I am wondering if the pfind is capable to do LFQ, and if yes, is the "match between runs" applied and…