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I am very interested in the development of human genetics. Genome editing, stopping aging at the genetic level and genetic modification.
**USSR Genetic**
I was surprised when I learned that in the U…
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This happened when @GC-repeat tried to import two Cas9 nickases in the CRISPR-Cas package.
Although everything was in order and several parts were already in the Cas variants library, the newly ad…
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I'm doing a standard Cas9 search on a 1 gigabase chicken genome and the step "Find genomic features closest to the guide" is taking longer than the guide finding step. I ran this on a 384 GB, 48-core…
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This is a reboot of #36 (solved in #40), with a few extra frills.
The classification system developed by [Makarova et al](http://dx.doi.org/10.1038/nrmicro3569) can be translated into a [decision t…
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Hello IGV team,
I am currently analyzing amplicons from an insect genomic DNA sample edited with CRISPR/Cas9. I am aware IGV offers insertion and deletion data by selecting a specific base pair of…
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Hey guys, just copying my intro over in a new introduction.
My name is Liam and I'm a first year MSc sctudent in the Pharmsci faculty. I did my undergrad at UBC and grew up in Vancouver. I love get…
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你好，非常棒的工作。我自己想重现一下文章的部分分析结果，我按照你的scRPBPBR流程获得了三个tsv文件，并且在GEO上获得了单细胞的表达矩阵。我在想怎么将两个信息合到一起呢（下面是处理的代码）。同时还有一个小问题哈，我看Crisper-Cas9基于的谱系示踪技术结果中都会绘制细胞的谱系发育树（当然可能不同的算法），在ploy-lox系统里面也能实现吗？如果能需要怎么做呢
![image](h…
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Hi there,
I tried to use uncalled to improve the efficiency of cas9 targeted sequencing on low inputs and it seems the reference I generated rejected the reads after ~1KB instead of continuing to …
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First, I would like to thank you for this amazing effort. This is by far the most convenient and complete command-line tool for Guide RNA design.
I am not sure if the scoring matrices are independen…