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Discusses qPCR, RNA sequencing, single-cell RNA sequencing, and 16s rRNA Gene (DNA) sequencing.
# @grabear's Checklist
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Templates have been used to create the following files:
- protocol_sampling_CTD.md
- protocol_DNA_extraction_Sterivex.md
- protocol_PCR_16S_V4V5.md
- protocol_PCR_16S_V9.md
- protocol_sequencin…
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The genome size of the species I'm assembling is 2.6Gb, and the ONT data comes from dorado sup v5.0.0 (R10).
When I used 167GB data (>Q7, >10Kb) with "Nanopore-UL-May2022.conf", I got an assembly o…
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For example, I have different time point scRNA sequencing data to explore the clonal structure of these samples. How do I choose the bulk DNA time point? And scRNA-seq and bulk DNA sampling from diffe…
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Hi,
I am using copykat to calculate CNV on a cancer sample, and results showed that not only cancer cells, but also there were a lot of macrophages with CNV.
Could macrophages have CNV in cancer sa…
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editor preferred term: Ion Torrent NGS Technology
alternative terms: Ion Torrent
textual definition: Ion Torrent is a semiconductor sequencing technology manufactured by Life Technologies which use…
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I’m slightly uncertain which DNA measures are optimal for the frequency based method (especially in case of samples containing animal tissues, where the proportion of microbial to animal DNA is often …
mniku updated
4 years ago
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Since the inclusion of OBI:0002144, the lab has posted a publication referencing circulome-seq.
http://www.g3journal.org/content/ggg/early/2017/08/11/g3.117.300141.full.pdf
Do you think it would b…
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> Next-generation sequencing (NGS) technologies have enabled affordable sequencing of billions of short DNA fragments at high throughput, paving the way for population-scale genomics. Genomics data an…