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I try to run the RNA-seq pipeline, but got this message:
```
Seq2science has trouble querying the assembly providers. Try again in a bit.
To see the error stack trace run seq2science with --debug…
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Hello!
I used Quast for times and know I am trying to use the rnaQUAST. Both tools are mentioned as great and robust quality assessment techniques.
Some thing are not clear enough to me to use rnaQU…
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+ [Application of a Novel “Pan-Genome”-Based Strategy for Assigning RNAseq Transcript Reads to Staphylococcus aureus Strains](https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0145861)…
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Dear team parsnp
I have a directory with **1320 assemblies genomes**. I run the command
parsnp -g ../../DB_all/genome_reference_VP/CP007004.gbk,../../DB_all/genome_reference_VP/CP007005.gbk -d…
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Hi,
I want to use criprVerse on a custom genome - a cell line- (no reference in public database like UCSC or NCBI)
I tried to adapt the tutorial "Design_Custom_Sequence" https://github.com/crisprVe…
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Develop a minimap2 alignment [see [docs](https://lh3.github.io/minimap2/minimap2.html)] function that uses minimap2 (installed via conda or in the PATH), we can run this so it produces a BAM file. The…
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I have just come across this behavior testing out mappy on a genome file that I had accidentally moved. `mappy.Aligner` is able to initialize with a non-existent file, but any operation (aside from th…
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```
hi, I use stamtools to deal with SAM form mosaik-aligner to find mutations.
and when i used the command :
samtools pileup -vcf ref.fa aln.bam | tee raw.txt | samtools.pl varFilter -D100
> flt.txt…
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Hi Alex,
I am using stereo-seq BGI mapping tool, which is using STAR aligner for barcode mapping and genome mapping.
I used --outSAMunmapped Within, to fetch the genome unmapped reads. However…
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when I run this:
singularity exec $pggb_path/pggb_latest.sif wfmash -t 20 $genome_path/ganganF73.genome.fa.gz --query-file-list=/home/user/huyang/shuai/data/pan_test/genome/genome.txt > aln.paf
I …