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I'm running STARsolo on FASTq files that were generated from data off a NovaSeq X sequencer. It isn't generating any filtered counts, which I realized is because the barcodes.tsv and matrix.tsv files …
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This issue is for keeping track of options for sequencing my DNA. Unfortunately, it seems all known methods require me to grant rights to my genes to the sequencing organization (including some copyr…
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I am trying to project a reference single-cell sequencing datasets onto a sorted cells microarray reference datasets. I subsetted the genes of the sc experiments to those available in the microarray d…
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Sorting by `projectDescription` fails to properly sort all results.
```
$ http 'https://service.azul.data.humancellatlas.org/index/projects?size=30&catalog=dcp37&order=asc&sort=projectDescription&…
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Hi there,
Thank you again for creating this wonderful tool! I had a question regarding your fusion gene analysis in your recent biorxiv paper: https://www.biorxiv.org/content/10.1101/2021.04.21.440…
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Discusses qPCR, RNA sequencing, single-cell RNA sequencing, and 16s rRNA Gene (DNA) sequencing.
# @grabear's Checklist
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Is the breadth -% transcriptome expressed in a sample- and the geometric mean -central tendency of a set of numbers- good measurements of the abundance of an organism?
We are interested into unders…
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Hi,
I have a few questions about the software.
1. I attempt to use siRNA-seq to generate the DGE result. Will different sequencing methods affect the data interpretation and accuracy?
2. I am …
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Hi, thanks for making IsoQuant, when i run my ont sequencing data, i got the following error. would you please kindly help to fix it. many thanks! :)
Command line: /home/cario/bin/miniconda3/envs…