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Hi Christoph,
Thanks for all your work.
When we use Scaledata we can specify the gene, for example"pbmc
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Hello,
Thank you very much for developing STAR/STARsolo. I am using it as default mapper for scRNA-Seq data. Since I am working on none model organism of which the gene annotation is frequently upd…
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Hello,
I have a problem when do the cell partition. Why use the cell_level_min_step1 = 2 but cell_level_min_step2 = 1 in the UMI counts matrix to do the cell partition?
hahia updated
4 years ago
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Hi,
After reading the tutorials carefully, I still feel confused how to prepare the input data. In most cases, users want to get cell-type fractions from tumor bulk RNA-seq data using the 10X data…
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Hi Alex,
I'm using the latest version (2.7.9a_2021-06-25) and, if I'm not mistaken, the definition of `Features.stats` file in the documentation doesn't match what is listed in the file.
For examp…
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Hi all,
The main result file of `velocyto` is a 4-layered loom file. I have read a loom file and found they are: `matrix`, `ambiguous`, `spliced`, and `unspliced`, what is the meaning of each layer…
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Hi Alex,
I am currently trying to do a splicing analysis of some SmartSeq single cell RNA-Seq data, aligned using StarSolo. During this, I have noticed a difference between the splice junction cou…
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Hi, sorry for asking again, but after going through the previous answers I have not found the answer that solves my problem. I have data that contains: HTOs, ADTs, 5' gex and VDJ. I'm trying to use C…
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Hi,
Thanks for providing a good tool.
I wonder if the **expression value unit** of **singlet and multiplet input data** must be **raw count**.
I have a normalized expression matrix. The normali…