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on page http://mimirna.centenary.org.au/irfinder/database/ the example form data when submitted throws error:
```
: [Errno 2] No such file or directory: '/srv/www/htdocs/irfinder/database/results/…
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Hi,
Thanks for providing such a amazing pipeline! I am trying to use your pipeline to process public RNAseq data such as SRR6880860. But after alignment, mmr keep running error, saying "ERROR: Header…
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Hello,
I have been using a pipeline consisting of PASA + BRAKER combining using EVM followed by two iterations of PASA update. The first run through of this worked fine for me when I did not specif…
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Hey,
it might be worthwhile to expose minimap2 parameters to the user in future versions. Much depends on the mapping accuracy and it could be helpful to fine tune the mapping especially for direct…
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Hi,
this is more a information request than a software issue.
I'd like to use `cellranger` for a custom genome reference. I know that my data will not map uniquely on that genome, I expect multipl…
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Dear , When I ran Hisat2 (version: 2.1.0) with --dta ( --downstream-transcriptome-assembly OR --dta-cufflinks), there was no XS tag in output file. I did not know how to fix it.
Thank you very much!
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Hi,
I have reads from direct cDNA sequencing that I would like call variants in. I tried using nanopolish, but it threw an error because it detected spliced alignments so It doesn't seem like it's…
ch728 updated
6 years ago
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I have been running nanopolish call-methylation on few nanopore data sets. On a certain dataset that contains some ultra long reads (some reads are close to 1 Mbases) I get the following error.
```…
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For several SE sequencing datasets, we find that Kallisto performs extremely poorly compared to Hisat2 in terms of total read alignments. For instance, in the example provided below, for a dataset wit…
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In the GRCh38 transcriptome you can get from Ensembl, there is at least one gene that is identical except for the fact that it appears both on the primary assembly and in an alternative locus. Ensembl…