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Hello,
I have followed the BaitSTR workflow to create 'contigs.str.fa' for one of my datasets. I am now trying to use BaitSTR_type and it looks to be getting hung up on creating an index. I think …
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I have all of the links in email. This is a great resource for testing new methods.
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I have been trying to assemble a 10Mb genome with uncorrected nanopore data (3-4 chromosomes expected). We have a lot of data, is that the reason Flye fails at the end?
[2019-06-22 11:00:05] INFO: …
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I am creating a workflow that has many steps that use different tools, and I'm creating [minimal Docker images](https://github.com/Niema-Docker) for each of the tools. I was wondering which of the fol…
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The `join` command currently just takes the union of positions and reads kept. While this is useful for joining non-overlapping amplicons, it will not treat primer binding sites correctly if the ampli…
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Hi,
I have an experiment wih a whitelist composed of 35 barcodes (60bp long, non standard technology in development) and when I try to run bustools correct I immediately obtain :
(/bioinfo/local…
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I have output from multiple sequencing runs that I want to analyze together, and some data from the same sequencing runs that I want to analyze separately. So my questions are:
1) should I combine da…
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Hello,
We have an amplicon dataset from a NovaSeq run and are exploring how we might alter settings in the dada2 pipeline to effectively identify errors in our data. In case you are unfamiliar, No…
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**Submitting author:** @wsmets (Wenke Smets)
**Repository:** https://github.com/LebeerLab/tidytacos
**Branch with paper.md** (empty if default branch): master
**Version:** v0.3.0
**Editor:** @diazrena…
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Hi,
I have output from different studies of 16S amplicon-sequenced reads with different sample sizes. They are from either V4 or V3-V4 regions but I want to analyze them together. I have run DADA2…
ghost updated
5 months ago