-
Hello,
Say I want to display a local HTML file (e.g. "/tmp/data/overview.html") which itself includes tags pointing to image files in same or child folder.
```
From IPython.core.display import …
-
### Description of feature
I know Ampliseq already uses fastqc, but I like using Seqkit stats when demultiplexing to track the number of reads that did and didn't get assigned to samples. There is an…
-
demultiplex.py should have an option to specify how to construct the sample and molecular barcodes. e.g.:
python demultiplex.py --sample_barcode i1:2-8,i2:2-8 --molecular_barcode i2:9-16 . . .
to s…
-
Hi everyone,
Until recently we used to apply Porechop after Albacore basecalling+demultiplexing. Now, we are using Guppy to basecall and we decided to apply Deepbinner to demultiplex.
My main q…
-
While running scHiCExplorer with tutorial, I have some questions about scHiCExplorer tools such as Demultiplex and BuildMatrix.
(The tutorial means Analysis of single-cell Hi-C data index)
At firs…
khsjh updated
1 month ago
-
I am demultiplexing four samples following [this vignette](https://satijalab.org/seurat/articles/hashing_vignette). The example data runs fine. When replacing with our data, I get this error in this p…
-
I am attempting to convert DIA files from a Fusion Lumos to mzML with MSConvert 3.0.23208-1321c92. When I attempt to convert, I get the error:
"Error writing run 1: SpectrumToIndices() Number of de…
-
`split_libraries.py` has the `-j` option which allows you to deal with duplicated barcodes across different "runs/lanes", however `split_libraries_fastq.py` does not include this option.
One of the t…
-
Hi,
I tried to run poreplex on a minION run using the follwing command to basecall and demultiplex barcodes from direct RNA sequencing:
` poreplex -i 20191122_1000_MN24929_FAL01495_e9928ec8/fast5/ -…
-
### Description of the bug
I've just upgraded sarek from 3.14 to the latest version. When I run it now it crashes out almost immediately with:
`ERROR ~ Cannot invoke method startsWith() on null ob…