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# Issue Report
## Please describe the issue:
Hello, I am currently performing target-specific custom barcode demultiplexing using Direct RNA seq data.
In the options, there is a setting for m…
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Hi folks,
Troubleshooting for a Moore lab member who is attempting to run a local transxpress run on our self-hosted computational server. The trimmomatic_parallel rule seems to have weird **writi…
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I ran the following command on my direct RNA sequencing data
```
tombo detect_modifications level_sample_compare \
--fast5-basedirs /native/singlefast5/ \
--alternate-fast5-basedirs /ivt/s…
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Please remove 'sncRNA' (SO:0002247) and 'sncRNA_gene' (SO:0002342) grouping classes.
These 'small non-coding RNA' terms are defined simply based on length ("a ncRNA less than 200 nucleotides") - es…
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This will be our first Remdesivir susceptibility data paper and a new table for chemical compound should be added.
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Dear developer,
I would like to ask whether Xron can be used for base-calling of RNA-sequencing data obtained from RNA004 direct RNA-Seq runs. Data are either *.fast5 or *.pod5.
Thanks and best
…
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Some questions
* Will FACS sorting disturb RNA?
* How should we preserve the cells post-sorting and pre-RNA isolation?
* How should we isolate RNA?
* What markers should we sort on?
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Update the following URL to point to the GitHub repository of
the package you wish to submit to _Bioconductor_
- Repository:https://github.com/Egors01/DuplexDiscovereR
Confirm the following by …
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Hello! Thanks for your helpful tool!
I am trying to assemble long-read sequencing data with short-read polishing. My long read data (nanopore direct RNA sequencing) are from the animals under 25 de…
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As discussed offline, our group has been trying to assess how to replicate/port our existing workflows to seqr.
We've found that the data that seqr allows us to enter about a given project/individ…