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Hi，
I am trying to run eventalign on reads from RNA004 and I get the following error:
[meth_main::13.631*0.41] 0 Entries (0.0M bases) loaded
[pthread_processor::13.631*0.41] 0 Entries (0.0M bases…
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- Consistently update set of reference transcripts for library source inference and include more genes for better accuracy
- Include organisms/sources from all clades in Ensembl: Bacteria, Plants, Fu…
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It would be nice to include a sort of mini -review of what we've learned at the site so far, along with a list of publications.
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Hi,
I tried to run dorado 0.7.0 on my older fast5 files converted to pod5 and I am still getting alot of unclassified reads:
![image](https://github.com/nanoporetech/dorado/assets/71407498/d1a82…
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Hello!
I wasn't sure where to post this, so feel free to move it if it's in the wrong place! Hello!
I'm trying to simulate barcoded reads with readfish and Icarust and running into an issue. W…
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# Issue Report
## Please describe the issue:
Hello, I've noticed that, after demultiplexing, some reads have missing values for the columns "template_start" and "template_duration" in the summar…
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**Submitting author:** @daylinmorgan (Daylin Morgan)
**Repository:** https://github.com/brocklab/pycashier
**Branch with paper.md** (empty if default branch): joss
**Version:** v2024.1006
**Editor:** …
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Hello,
i'm currently dealing with RNA-seq data generated with Lexogen QuantSeq, as ana laternative method to microarrays (https://www.lexogen.com/quantseq-3mrna-sequencing/). Briefly, it's a kit to g…
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Thank you for the nice work flow published on F1000: https://f1000research.com/articles/9-1263/v1
Question 1:
I am able to successfully run the R code upto the code below:
```
# split SCE b…
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Hi,
Small question regarding the automatic model selection with dorado.
I ran sequencing at 260 bases per second but dorado appears to select 400bps for basecalling.
Fyi, the POD5 (using ins…